Evaluation of prebiotic activities of Salvia species as potential functional components for intestinal health

Mevlüde Nur Dilgen; Betül Aydın; Murat Koç

doi:10.21448/ijsm.1764418

Evaluation of prebiotic activities of Salvia species as potential functional components for intestinal health

Abstract

Some Salvia species have long been used in traditional medicine for digestive disorders. This study investigated the prebiotic potential of water and ethanol extracts of Salvia fruticosa, S. huberi, S. sclarea, and S. aethiopis on probiotic bacteria Lactobacillus fermentum ATCC 14931 and L. plantarum ATCC 8014. Among the tested species, the extracts of S. huberi and S. sclarea exhibited the highest prebiotic activity. The ethanol extract of S. huberi showed prebiotic activity scores comparable to commercial prebiotics. Under simulated gastrointestinal conditions, the S. huberi water extract significantly increased the survival of L. fermentum, while the S. sclarea water and ethanol extracts provided the highest viability rate for L. plantarum. Both species provided the highest cell surface hydrophobicity values and strong post-fermentation antioxidant activity; S. huberi water extract reached the radical scavenging rate. The present in vitro findings suggest that S. huberi and S. sclarea are promising candidates with potential prebiotic properties that may contribute to probiotic viability and gut health.

Keywords

Supporting Institution

None.

Project Number

None.

References

Ab Azid, S.M, Wichienchot, S, Ishak, W.R.W, & Safuan, S. (2024). Prebiotic activity of plants from cucurbitaceae family and in vitro fermentation by Gut Microbiota. Jurnal Gizi dan Pangan, 19(Supp. 2), 342-351. https://doi.org/10.25182/jgp.2024.19.Supp.2.342-351
Al-Habsi, N., Al-Khalili, M., Haque, S.A., Elias, M., Olqi, N.A., & Al Uraimi, T. (2024). Health benefits of prebiotics, probiotics, synbiotics, and postbiotics. Nutrients, 16(22), 3955. https://doi.org/10.3390/nu16223955
Aydın, B. (2024). Exploring the multifaceted health benefits of Tripolium pannonicum: Antimicrobial, antioxidant, and prebiotic properties with phytochemical insights. Chemistry & Biodiversity, 22(2), e202402588. https://doi.org/10.1002/cbdv.202402588
Aziz, T., Xingyu, H., Sarwar, A., Naveed, M., Shabbir, M.A., Khan, A.A., & Jalal, R.S. (2023). Assessing the probiotic potential, antioxidant, and antibacterial activities of oat and soy milk fermented with Lactiplantibacillus plantarum strains isolated from Tibetan Kefir. Frontiers Microbiology, 14, 1265188. https://doi.org/10.3389/fmicb.2023.1265188
Bayram, O.Y., Kinik, O., & Büyükkileci, C. (2024) Antimicrobial activity and sensory acceptability of probiotic-strained (Torba) yogurt with medicinal and aromatic plants. South African Journal of Botany, 174, 218-227. https://doi.org/10.1016/j.sajb.2024.08.053
Braca, A., De Tommasi, N., Di Bari, L., Pizza, C., Politi, M., & Morelli, I. (2001) Antioxidant principles from Bauhinia tarapotensis. Journal of Natural Products, 64(7), 892 895. https://doi.org/10.1021/np0100845
Castorena-Alba, M. M., Vázquez-Rodríguez, J. A., López-Cabanillas Lomelí, M., & González-Martínez, B. E. (2018). Cholesterol assimilation, acid and bile survival of probiotic bacteria isolated from food and reference strains. CyTA Journal of Food, 16(1), 36 41. https://doi.org/10.1080/19476337.2017.1335347
Chae, Y.R., Lee, H.B., Lee, Y.R., Yoo, G., Lee, E., Park, M., & Park, H.Y. (2024) Ameliorating effects of Orostachys japonica against high-fat diet-induced obesity and gut dysbiosis. Journal Ethnopharmacology, 333, 118443.

Cui, H., Zhang, X., Zhou, H., Zhao, C., & Lin, L. (2015) Antimicrobial activity and mechanisms of Salvia sclarea essential oil. Botanical Studies, 56, 1-8. https://doi.org/10.1186/s40529-015-0096-4
Davani-Davari, D., Negahdaripour, M., Karimzadeh, I., Seifan, M., Mohkam, M., Masoumi, S., Berenjian, A, & Ghasemi, Y. (2019). Prebiotics: Definition, Types, Sources, Mechanisms, and Clinical Applications. Foods, 8. https://doi.org/10.3390/foods8030092
Delgado, S., Guadamuro, L., Flórez, A.B., Vázquez, L., & Mayo, B. (2019). Fermentation of commercial soy beverages with lactobacilli and bifidobacteria strains featuring high β-glucosidase activity. IFSET, 51, 148-155. https://doi.org/10.1016/j.ifset.2018.03.018
Dilgen, M.N., Aydın, B., Vural, N., & Koç, M. (2025). Analysis of phytochemical profiles of different extracts of Salvia (S. sclarea, S. huberi, S. fruticosa and S. aethiopis) by HPLC‐PDA, evaluation of their antioxidant capacity and antimicrobial activity. Chemistry & Biodiversity, e03218. https://doi.org/10.1002/cbdv.202403218
Dimitrellou, D., Moschakis, T., & Kandylis, P. (2025). Impact of apple pulp on textural characteristics, microstructure, volatile profile, and sensory acceptance of yogurts. Foods, 14(14), 2453. https://doi.org/10.3390/foods14142453
Farid, W., Masud, T., Sohail, A., Ahmad, Naqvi, N.S.S.S., Khan, A.A., Khalifa, S.A., Hussain, A., & Ali, S. (2021). Gastrointestinal transit tolerance, cell surface hydrophobicity, and functional attributes of Lactobacillus Acidophilus strains isolated from Indigenous Dahi. Food Science Nutrition. 9, 5092-5102. https://doi.org/10.1002/fsn3.2468
Feng, T., & Wang, J. (2020). Oxidative stress tolerance and antioxidant capacity of lactic acid bacteria as probiotic: a systematic review. Gut microbes, 12(1), 1801944. https://doi.org/10.1080/19490976.2020.1801944
Figueroa-Gonzalez, I., Rodriguez-Serrano, G., Gomez-Ruiz, L., Garcia-Garibay, M., & Cruz-Guerrero, A. (2019). Prebiotic effect of commercial saccharides on probiotic bacteria isolated from commercial products. Food Science Technology International, 39(3), 747-753. https://doi.org/10.1590/fst.07318
Gibson, G., & Roberfroid, M. (1995). Dietary modulation of the human colonic microbiota introducing the concept of prebiotics. Journal Nutrition, 125(6), 1401 12. https://doi.org/10.1093/JN/125.6.1401
Gul, S., & Durante-Mangoni, E. (2024). Unraveling the Puzzle: Health Benefits of Probiotics A Comprehensive Review. Journal Clinical Medicine, 13(5), 1436. https://doi.org/10.3390/jcm13051436
Haritash, A.K., & Kaushik, C.P. (2009). Biodegradation aspects of polycyclic aromatic hydrocarbons (PAHs): a review. Journal of Hazardous Materials, 169(1), 1 15. https://doi.org/10.1016/j.jhazmat.2009.03.137
Holkem, A.T., Silva, M.P.D., Favaro-Trindade, C.S., (2023). Probiotics and plant extracts: A promising synergy and delivery systems. Critical Reviews in Food Science and Nutrition, 63(28), 9561-9579. https://doi.org/10.1080/10408398.2022.2066623
Huebner, J., Wehling, R.L., Hutkins, R.W., (2007). Functional activity of commercial prebiotics. International Dairy Journal, 17(7), 770-775. https://doi.org/10.1016/j.idairyj.2006.10.006
Jankute, M., Nataraj, V., Lee, O.Y.C., Wu, H.H., Ridell, M., Garton, N.J., Besra, G.S. (2017). The role of hydrophobicity in tuberculosis evolution and pathogenicity. Scientific Reports, 7(1), 1315.
Jan, S.K., & Kumar, A.N. (2022) Effect of Diverse Fermentation Treatments on Nutritional Composition, Bioactive Components, and Anti- Nutritional Factors of Finger Millet (Eleusine coracana L.). Journal Biotechnology, 10, 46–52. https://doi.org/10.7324/JABB.2022.10s107
Kim, S., Lee, J.Y., Jeong, Y., Kang, C.H. (2022). Antioxidant activity and probiotic properties of lactic acid bacteria. Fermentation. 8, 29. https://doi.org/10.3390/fermentation8010029
Latif, A., Shehzad, A., Niazi, S., Zahid, A., Ashraf, W., Iqbal, M.W., Korma, S.A. (2023). Probiotics: Mechanism of action, health benefits and their application in food industries. Front Microbiology, 14, 1216674. https://doi.org/10.3389/fmicb.2023.1216674
Lau, L.Y.J., & Quek, S.Y. (2024). Probiotics: Health benefits, food application, and colonization in the human gastrointestinal tract. Food Biotechnology, 3(1), 41-64. https://doi.org/10.1002/fbe2.12078
Lau, L.Y.J., Huang, K., & Quek, S.Y. (2025). Impact of phenolic-rich feijoa (Acca sellowiana'Kakariki') peel extract on the mucin-adhesive properties of selected probiotic strains. Food Bioscience, 106320. https://doi.org/10.1016/j.fbio.2025.106320
Maftei, N.M., Raileanu, C.R., Balta, A.A., Ambrose, L., Boev Marin, D.B., & Lisa, E.L. (2024). The Potential Impact of Probiotics on Human Health: An Update on Their Health-Promoting Properties. Microorganisms, 12(2), 234. https://doi.org/10.3390/microorganisms12020234
Mashudin, M., Hasham, R., Cheng, K.K., Leong, H.Y., Othman, N.Z., Shadan, N.H., & Hamzah, M.A.A. (2024). Prebiotic effects of extracts from pineapple and Curcuma xanthorrhiza rhizomes towards the growth of Lacticaseibacillus paracasei. Food Bioscience, 59, 104045. https://doi.org/10.1016/j.fbio.2024.104045
Milutinović, M., Dimitrijević-Branković, S., & Rajilić-Stojanović, M. (2021). Plant extracts rich in polyphenols as potent modulators in the growth of probiotic and pathogenic intestinal microorganisms. Front Nutrition, 8, 688843. https://doi.org/10.3389/fnut.2021.688843
Morais, S.G.G., Borges, G.D.S.C, dos Santos Lima, M., Martín-Belloso, O., & Magnani, M. (2019). Effects of probiotics on the content and bioaccessibility of phenolic compounds in red pitaya pulp. International Food Resistance, 126, 108681. https://doi.org/10.1016/j.foodres.2019.108681
Murugesan, V., Palanivel, P., Ramesh, G., Ganesh, D., Michael, H.S.R., Bandhumy, Lingam., S., & Sivaraman, R.K. (2025). Exploring the antibacterial potential of Clidemia hirta leaf extract against the pathogenicity of Pseudomonas aeruginosa: in vitro and in silico approaches. Front Pharmacology, 16,1555542. https://doi.org/10.3389/fphar.2025.1555542
Putri, D.N., Widyarti, S., & Jatmiko, Y.D. (2022). Increasing antioxidant activity of soursop (Annona muricata L.) and noni (Morinda citrifolia L.) leaves fermented by Lactobacillus plantarum BP102. Berk Penelit Hayati 27, 73-77. https://doi.org/10.23869/bphjbr.27.2.20223.
Quldchikh, S., TirTouil, A., & Meddah, B. (2020). Zizyphus lotus (L.) extracts as prebiotics in the aggregation and adhesion of probiotic and inhibition of pathologic bacteria from patients with colorectal cancer. Journal Food Studies, 9(1). https://doi.org/10.7455/ijfs/9.1.2020.a3
Raddatz, G.C., Fonseca, V.R., Cichoski, A.J., Zepka, L.Q., Jacob-Lopes, E., Campagnol, P.C.B., & de Menezes, C.R. (2022). Viability and stability evaluation of Lactobacillus casei LC03 co-encapsulated with red onion (Allium cepa L.) peel extract. LWT, 153,112434. https://doi.org/10.1016/j.lwt.2021.112434
Rani, S., & Anthony, U. (2014). Effect of Germination and Fermentation on Polyphenols in Finger Millet (Eleusine coracana). International Journal of Food and Nutritional Sciences, 3, 65–68.
Rastall, R., & Gibson, G. (2015). Recent developments in prebiotics to selectively impact beneficial microbes and promote intestinal health. Current Opinion in Biotechnology, 32, 42-46. https://doi.org/10.1016/j.copbio.2014.11.002
Russo, A., Formisano, C., & Rigano, D. (2013). Chemical composition and anticancer activity of essential oils of Mediterranean sage (Salvia officinalis L.) grown in different environmental conditions. Food Chemical Toxicology, 55, 42–47. https://doi.org/10.1016/j.fct.2012.12.036
Rodríguez-Sojo, M.J., Ruiz-Malagón, A.J., Hidalgo-García, L., Molina-Tijeras, J.A., Diez-Echave, P., López-Escanez, L., & Rodríguez-Nogales, A. (2023). The Prebiotic Effects of an Extract with Antioxidant Properties from Morus alba L. Contribute to Ameliorate High-Fat Diet-Induced Obesity in Mice. Antioxidants, 12(4), 978. https://doi.org/10.3390/antiox12040978
Sadeghi, M., Panahi, B., Mazlumi, A., Hejazi, M.A., Komi, D.E.A., & Nami, Y. (2022). Screening of potential probiotic lactic acid bacteria with antimicrobial properties and selection of superior bacteria for application as biocontrol using machine learning models. Lebensm Wiss Technolology, 162, 113471. https://doi.org/10.1016/j.lwt.2022.113471
Singleton, V.L., & Rossi, J.A. (1965). Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American Journal of Botany, 16(3), 144 158. https://doi.org/10.5344/ajev.1965.16.3.144
Shargh, M.S., Dastar, B., Zerehdaran, S., Khomeiri, M., & Moradi, A. (2012). Effects of using plant extracts and a probiotic on performance, intestinal morphology, and microflora population in broilers. Journal Applied Poultry, 21(2), 201-208. https://doi.org/10.3382/japr.2010-00145
Shehata, M.G., Abd-Rabou, H.S., & El-Sohaimy, S.A. (2019). Plant extracts in probiotic encapsulation: evaluation of their effects on strains survivability in juice and drinkable yogurt during storage and an in vitro gastrointestinal model. Journal of Pure and Applied Microbiology, 13, 1 https://dx.doi.org/10.22207/JPAM.13.1.70
Skenderidis, P., Mitsagga, C., Lampakis, D., Petrotos, K., & Giavasis, I. (2019). The effect of encapsulated powder of goji berry (Lycium barbarum) on growth and survival of probiotic bacteria. Microorganisms, 8(1), 57. https://doi.org/10.3390/microorganisms8010057
Stasiak-Różańska, L., Berthold-Pluta, A., Pluta, A.S., Dasiewicz, K., & Garbowska, M. (2021). Effect of simulated gastrointestinal tract conditions on survivability of probiotic bacteria present in commercial preparations. International Journal of Environmental Research and Public Health, 18(3), 1108. https://doi.org/10.3390/ijerph18031108
Succi, M., Tremonte, P., Pannella, G., Tipaldi, L., Cozzolino, A., Coppola, R., & Sorrentino, E. (2017). Survival of commercial probiotic strains in dark chocolate with high cocoa and phenols content during the storage and in a static in vitro digestion model. Journal of Function Foods, 35, 60–67. https://doi.org/10.1016/j.jff.2017.05.019.ü
Sutherland, J., Miles, M., Hedderley, D., Li, J., Devoy, S., Sutton, K., & Lauren, D. (2009). In vitro effects of food extracts on selected probiotic and pathogenic bacteria. International Journal Food Science Nutritional, 60(8), 717-727. https://doi.org/10.3109/09637480802165650.
Thuaytong, W., & Anprung, P. (2011). Bioactive compounds and prebiotic activity in Thailand-grown red and white guava fruit (Psidium guajava L.). Food Science Technology International, 17(3), 205-212. https://doi.org/10.1177/1082013210382066
Udeh, H.O., Duodu, K.G., & Jideani, A.I.O. (2025). Effect of Fermentation Time on the Physicochemical Properties and Phenolic Composition of Malted Finger Millet Beverages. Food Science Nutrition, 13(3), e4659. https://doi.org/10.1002/fsn3.4659
Valero-Cases, E., Nuncio-Jáuregui, N., & Frutos, M.J. (2017). Influence of fermentation with different lactic acid bacteria and in vitro digestion on the biotransformation of phenolic compounds in fermented pomegranate juices. Journal Agriculttural Food Chemistry, 65(31), 6488-6496. https://doi.org/10.1021/acs.jafc.6b04854
Van Engeland, C., Haut, B., & Debaste, F. (2025). A closer look at the potential mechanisms of action of protective agents used in the drying of microorganisms: A review. Microorganisms, 13(2), 435. https://doi.org/10.3390/microorganisms13020435

Details

Primary Language

English

Subjects

Plant Biochemistry

Journal Section

Research Article

Authors

Mevlüde Nur Dilgen ^*
0000-0002-9720-8662
Türkiye

Betül Aydın
0000-0002-9092-1350
Türkiye

Murat Koç
0000-0002-0829-4571
Türkiye

Early Pub Date

July 25, 2026

Publication Date

September 2, 2026

Submission Date

August 14, 2025

Acceptance Date

April 28, 2026

Published in Issue

Year 2026 Volume: 13 Number: 3

DOI

https://doi.org/10.21448/ijsm.1764418

IZ

https://izlik.org/JA89PT26HB

Cite

RIS / Bibtex

APA

Dilgen, M. N., Aydın, B., & Koç, M. (2026). Evaluation of prebiotic activities of Salvia species as potential functional components for intestinal health. International Journal of Secondary Metabolite, 13(3), 666-682. https://doi.org/10.21448/ijsm.1764418

AMA

1.Dilgen MN, Aydın B, Koç M. Evaluation of prebiotic activities of Salvia species as potential functional components for intestinal health. Int. J. Sec. Metabolite. 2026;13(3):666-682. doi:10.21448/ijsm.1764418

Chicago

Dilgen, Mevlüde Nur, Betül Aydın, and Murat Koç. 2026. “Evaluation of Prebiotic Activities of Salvia Species As Potential Functional Components for Intestinal Health”. International Journal of Secondary Metabolite 13 (3): 666-82. https://doi.org/10.21448/ijsm.1764418.

EndNote

Dilgen MN, Aydın B, Koç M (September 1, 2026) Evaluation of prebiotic activities of Salvia species as potential functional components for intestinal health. International Journal of Secondary Metabolite 13 3 666–682.

IEEE

[1]M. N. Dilgen, B. Aydın, and M. Koç, “Evaluation of prebiotic activities of Salvia species as potential functional components for intestinal health”, Int. J. Sec. Metabolite, vol. 13, no. 3, pp. 666–682, Sept. 2026, doi: 10.21448/ijsm.1764418.

ISNAD

Dilgen, Mevlüde Nur - Aydın, Betül - Koç, Murat. “Evaluation of Prebiotic Activities of Salvia Species As Potential Functional Components for Intestinal Health”. International Journal of Secondary Metabolite 13/3 (September 1, 2026): 666-682. https://doi.org/10.21448/ijsm.1764418.

JAMA

1.Dilgen MN, Aydın B, Koç M. Evaluation of prebiotic activities of Salvia species as potential functional components for intestinal health. Int. J. Sec. Metabolite. 2026;13:666–682.

MLA

Dilgen, Mevlüde Nur, et al. “Evaluation of Prebiotic Activities of Salvia Species As Potential Functional Components for Intestinal Health”. International Journal of Secondary Metabolite, vol. 13, no. 3, Sept. 2026, pp. 666-82, doi:10.21448/ijsm.1764418.

Vancouver

1.Mevlüde Nur Dilgen, Betül Aydın, Murat Koç. Evaluation of prebiotic activities of Salvia species as potential functional components for intestinal health. Int. J. Sec. Metabolite. 2026 Sep. 1;13(3):666-82. doi:10.21448/ijsm.1764418