The augmentation in apoptosis and cell cycle-associated transcriptional gene expression in A549 cells induced by Polyspora huongiana leaves methanol extract

Nguyen Trung Quan; Tran Thi; Bui Thi Kim Ly; Hoang Thanh Chi

doi:10.21448/ijsm.1788049

The augmentation in apoptosis and cell cycle-associated transcriptional gene expression in A549 cells induced by Polyspora huongiana leaves methanol extract

Abstract

According to the WHO, in 2020, lung cancer was recorded as the second most common new case of cancer in the world, and it causes the most deaths among cancer types. The side effects during the treatment are considerable, so novel therapies are being proposed and research broadened. Research on medicinal herbs, including tea, in cancer treatment always brings breakthrough discoveries. Although Polyspora huongiana was described many years ago, its scientific data on the ability to treat lung cancer have still been mystified. The cytotoxicity assay and the wound healing evaluation combination were performed to investigate the cytotoxicity of the P. huongiana leaf extract on lung cancer cells, A549. The flow cytometry assay and the transcriptomic level measurement were experimented with to clarify the action primarily. The results showed that the extract inhibited cell growth with an IC50 of 195.80 ± 25.00 µg/mL, and the apoptotic cells were observed. The apoptosis and cell cycle-regulated gene escalation were recorded in A549 under treatment with the P. huongiana extract.

Keywords

Thanks

We thank Mr Truong Quang Cuong and Mr Phung My Trung for the sample collection and identification

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Details

Primary Language

English

Subjects

Plant Biotechnology, Biochemistry and Cell Biology (Other)

Journal Section

Research Article

Authors

Nguyen Trung Quan
0000-0002-6436-4693
Vietnam

Tran Thi
0009-0005-6732-2930
Vietnam

Bui Thi Kim Ly
0000-0002-8433-7035
Vietnam

Hoang Thanh Chi ^*
0000-0002-6638-1235
Vietnam

Early Pub Date

June 8, 2026

Publication Date

June 9, 2026

Submission Date

September 20, 2025

Acceptance Date

March 27, 2026

Published in Issue

Year 2026 Volume: 13 Number: 2

DOI

https://doi.org/10.21448/ijsm.1788049

IZ

https://izlik.org/JA94LR46WK

Cite

RIS / Bibtex

APA

Quan, N. T., Thi, T., Thi Kim Ly, B., & Thanh Chi, H. (2026). The augmentation in apoptosis and cell cycle-associated transcriptional gene expression in A549 cells induced by Polyspora huongiana leaves methanol extract. International Journal of Secondary Metabolite, 13(2), 565-574. https://doi.org/10.21448/ijsm.1788049

AMA

1.Quan NT, Thi T, Thi Kim Ly B, Thanh Chi H. The augmentation in apoptosis and cell cycle-associated transcriptional gene expression in A549 cells induced by Polyspora huongiana leaves methanol extract. Int. J. Sec. Metabolite. 2026;13(2):565-574. doi:10.21448/ijsm.1788049

Chicago

Quan, Nguyen Trung, Tran Thi, Bui Thi Kim Ly, and Hoang Thanh Chi. 2026. “The Augmentation in Apoptosis and Cell Cycle-Associated Transcriptional Gene Expression in A549 Cells Induced by Polyspora Huongiana Leaves Methanol Extract”. International Journal of Secondary Metabolite 13 (2): 565-74. https://doi.org/10.21448/ijsm.1788049.

EndNote

Quan NT, Thi T, Thi Kim Ly B, Thanh Chi H (June 1, 2026) The augmentation in apoptosis and cell cycle-associated transcriptional gene expression in A549 cells induced by Polyspora huongiana leaves methanol extract. International Journal of Secondary Metabolite 13 2 565–574.

IEEE

[1]N. T. Quan, T. Thi, B. Thi Kim Ly, and H. Thanh Chi, “The augmentation in apoptosis and cell cycle-associated transcriptional gene expression in A549 cells induced by Polyspora huongiana leaves methanol extract”, Int. J. Sec. Metabolite, vol. 13, no. 2, pp. 565–574, June 2026, doi: 10.21448/ijsm.1788049.

ISNAD

Quan, Nguyen Trung - Thi, Tran - Thi Kim Ly, Bui - Thanh Chi, Hoang. “The Augmentation in Apoptosis and Cell Cycle-Associated Transcriptional Gene Expression in A549 Cells Induced by Polyspora Huongiana Leaves Methanol Extract”. International Journal of Secondary Metabolite 13/2 (June 1, 2026): 565-574. https://doi.org/10.21448/ijsm.1788049.

JAMA

1.Quan NT, Thi T, Thi Kim Ly B, Thanh Chi H. The augmentation in apoptosis and cell cycle-associated transcriptional gene expression in A549 cells induced by Polyspora huongiana leaves methanol extract. Int. J. Sec. Metabolite. 2026;13:565–574.

MLA

Quan, Nguyen Trung, et al. “The Augmentation in Apoptosis and Cell Cycle-Associated Transcriptional Gene Expression in A549 Cells Induced by Polyspora Huongiana Leaves Methanol Extract”. International Journal of Secondary Metabolite, vol. 13, no. 2, June 2026, pp. 565-74, doi:10.21448/ijsm.1788049.

Vancouver

1.Nguyen Trung Quan, Tran Thi, Bui Thi Kim Ly, Hoang Thanh Chi. The augmentation in apoptosis and cell cycle-associated transcriptional gene expression in A549 cells induced by Polyspora huongiana leaves methanol extract. Int. J. Sec. Metabolite. 2026 Jun. 1;13(2):565-74. doi:10.21448/ijsm.1788049