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Biodiversity of Fungi in Strawberry Fields in Anamur, TURKEY

Yıl 2018, Cilt 5, Sayı 1, 20 - 26, 04.01.2018
https://doi.org/10.21448/ijsm.346209

Öz

Strawberry is a delicious and aromatic fruit, which can be consumed as fresh and also is suitable for industry. However, strawberry is exposed to many fungal diseases that end with the loss of the product up to % 15 before harvest. The aim of this study is to determine the fungi that present in the field whether or not pathogenic. Samples were collected from different strawberry fields in Anamur in April 2016. Morphological identification was made according to the shape and color of the colonies, mycelium and spore structures. For molecular identification, ITS gene region was used. According to morphological and molecular methods, seven different fungal genera were found on strawberries.

Kaynakça

  • Reddy, C.S., Ghai, R., Rashmi, K., Kalai, V.C. (2003). Polyhydroxyalkanoates: an overview. Bioresource Technol., 87(2), 137-146.
  • Ikhiwili, O.M. (2012). Isolation and Characterisation of Microorganisms Associated with Rot Diseases of Fruit, Stem and Leaf of Carica papaya L. A Project Report Submitted to the Department of Biological Sciences, College of Science and Technology, Covenant University, Canaanland, Ota, Ogun state, Nigeria. 5-6.
  • Singh, D., Sharma, R.R. (2007). Postharvest diseases of fruit and vegetables and their management. In: Prasad, D., editor. Sustainable Pest Management. Daya Publishing House, New Delhi, India.
  • Al-Hindi, R.R., Al-Najada, A.R., & Mohamed, S.A. (2011). Isolation and identification of some fruit spoilage fungi: Screening of plant cell wall degrading enzymes. Afr. J. Microbiol. Res., 5(4), 443-448.
  • Droby, S. (2006). Improving quality and safety of fresh fruits and vegetables after harvest by the use of biocontrol agents and natural materials. Acta Hortic., 709, 45-51.
  • Mailafia, S., Okoh, G.R., Olabode H.O.K, Osanupin, R. (2017). Isolation and identification of fungi associated with spoilt fruits vended in Gwagwalada market, Abuja, Nigeria. Vet. World, 10(4), 393-397.
  • Hafez, E.E., Elbestawy, E. (2008). Molecular Chracterization of Soil Microorganisms: effect of industrial pollution on distribution and biodiversity. World J Microbiol Biotechnol. 25, 215-224.
  • Bardgett, R.D. (2005). The biology of soil: a community and ecosystem approach. Oxford University Press Inc, New York.
  • Hawksworth, D.L. (2001). The magnitude of fungal diversity: the 1.5 million species Estimate. Mycol. Res., 105(12), 1422-1432.
  • Abdullah, Q., Mahmoud, A., Al-harethi, A., (2016). Isolation and Identification of Fungal Post-harvest Rot of Some Fruits in Yemen. PSM Microbiol., 1(1), 36-44.
  • Jensen, B., Knudsen, I.M., Andersen, B., Nielsen, K.F., Thrane, U., Jensen, D.F., & Larsen, J. (2013). Characterization of microbial communities and fungal metabolites on field grown strawberries from organic and conventional production. Int. J. Food microbiol., 160(3), 313-322.
  • Samson, R.A., Hoekstra, E.S. and Frisvad, J.C. (2004). Introduction to Food-and Airborne Fungi, Laboratory Manual Series 2, Food and Indoor Fungi, 389pp.
  • Doyle J.J., Doyle J.L. (1987). Isolation of Plant DNA From Fresh Tissue. Focus, 12, 13-15.
  • Boysen M., Skoube P., Frisvad, J., Rossen, L. (1996). Reclassification of the Penicillium roqueforti group into three species on the basis of molecular genetic and biochemical profiles. Microbiol., 142, 541-519.
  • Baiyewu, R.A., Amusa, N.A., Ayoola, O.A., & Babalola, O.O. (2007). Survey of the postharvest diseases and aflatoxin contamination of marketed Pawpaw fruit (Carica papaya L.) in South Western Nigeria. Afr. J. Agric. Res., 2(4), 178-181.
  • Chukwuka, K.S., Okonko, I.O., & Adekunle, A.A. (2010). Microbial ecology of organisms causing pawpaw (Carica papaya L.) fruit decay in Oyo State, Nigeria. Am. Eurasian J. Toxicol. Sci., 2(1), 43-50.
  • Staats, M., Baarlen, P.V., Kan, J.A.V. (2004). Molecular Phylogeny of the Plant Pathogenic Genus Botrytis and the Evolution of Host Specificity. Mol. Bio. Evol., 22(2), 333-346.
  • Kasiamdari, R.S., Smith, E.S., Scott, E.S., Smith, F.A. (2002). Identification of binucleate Rhizoctonia as a contaminant in pot cultures of arbuscular mycorrhizal fungi and development of a PCR-based method of detection. Mycol. Res. 106 (12), 1417-1426.
  • Khairnar, D.N., Kelhe, A.S., Khairnar, A.B. (2011). Soil-borne Fungal Biodiversity of Some Fruit Crops of Nashik District and Control Measures. Nat. Envir. Pollution Tech., 10(1), 127-128.
  • Abdelfattah, A, Li Destri Nicosia, M.G, Cacciola S.O., Droby, S, Schena, L. (2015). Metabarcoding Analysis of Fungal Diversity in the Phyllosphere and Carposphere of Olive (Olea europaea). PLoS ONE, 10(7), e0131069.
  • Van Hemelrijck, W., Ceustermans, A., Van Campenhout, J., Lieten, P., Bylemans, D. (2017). Crown rot in strawberry caused by Pestalotiopsis. Acta Horticul., 1156, 781-786.
  • Zhao, J.N., Ma,, Z., Liu, Z.P. (2016). Pestalotiopsis clavispora causing leaf spot on strawberry. Mycosystema, 35(1), 114-120.
  • Adrian, M., Jeandet, P., Veneau, J., Weston, L.A., & Bessis, R. (1997). Biological activity of resveratrol, a stilbenic compound from grapevines, against Botrytis cinerea, the causal agent for gray mold. J. Chem. Eco., 23(7), 1689-1702.
  • Lagopodi, A.L., & Thanassoulopoulos, C.C. (1998). Effect of a leaf spot disease caused by Alternaria alternata on yield of sunflower in Greece. Plant Disease, 82(1), 41-44.
  • Johnson, G. I., Sangchote, S., & Cooke, A. W. (1990). Control of stem end rot (Dothiorella dominicana) and other postharvest diseases of mangoes (cv. Kensington Pride) during short-and long-term storage. Tropic. Agricult. Trinidad and Tobago, 67(2), 183-187.
  • Narasimhan, M.L., Damsz, B., Coca, M.A., Ibeas, J.I., Yun, D.J., Pardo, J.M., & Bressan, R.A. (2001). A plant defense response effector induces microbial apoptosis. Mol. Cell, 8(4), 921-930.
  • Menkis, A., Vasiliauskas, R., Taylor, A.F.S., Stenström, E., Stenlid, J., & Finlay, R. (2006). Fungi in decayed roots of conifer seedlings in forest nurseries, afforested clear‐cuts and abandoned farmland. Plant Patho., 55(1), 117-129.
  • Di Pietro, A., García‐Maceira, F. I., Meglecz, E., & Roncero, M.I.G. (2001). A MAP kinase of the vascular wilt fungus Fusarium oxysporum is essential for root penetration and pathogenesis. Mol. Microbiology, 39 (5), 1140-1152.

Biodiversity of Fungi in Strawberry Fields in Anamur, TURKEY

Yıl 2018, Cilt 5, Sayı 1, 20 - 26, 04.01.2018
https://doi.org/10.21448/ijsm.346209

Öz

Strawberry is a delicious and aromatic fruit, which can be consumed as fresh and also is suitable for industry. However, strawberry is exposed to many fungal diseases that end with the loss of the product up to % 15 before harvest. The aim of this study is to determine the fungi that present in the field whether or not pathogenic. Samples were collected from different strawberry fields in Anamur in April 2016. Morphological identification was made according to the shape and color of the colonies, mycelium and spore structures. For molecular identification, ITS gene region was used. According to morphological and molecular methods, seven different fungal genera were found on strawberries.

Kaynakça

  • Reddy, C.S., Ghai, R., Rashmi, K., Kalai, V.C. (2003). Polyhydroxyalkanoates: an overview. Bioresource Technol., 87(2), 137-146.
  • Ikhiwili, O.M. (2012). Isolation and Characterisation of Microorganisms Associated with Rot Diseases of Fruit, Stem and Leaf of Carica papaya L. A Project Report Submitted to the Department of Biological Sciences, College of Science and Technology, Covenant University, Canaanland, Ota, Ogun state, Nigeria. 5-6.
  • Singh, D., Sharma, R.R. (2007). Postharvest diseases of fruit and vegetables and their management. In: Prasad, D., editor. Sustainable Pest Management. Daya Publishing House, New Delhi, India.
  • Al-Hindi, R.R., Al-Najada, A.R., & Mohamed, S.A. (2011). Isolation and identification of some fruit spoilage fungi: Screening of plant cell wall degrading enzymes. Afr. J. Microbiol. Res., 5(4), 443-448.
  • Droby, S. (2006). Improving quality and safety of fresh fruits and vegetables after harvest by the use of biocontrol agents and natural materials. Acta Hortic., 709, 45-51.
  • Mailafia, S., Okoh, G.R., Olabode H.O.K, Osanupin, R. (2017). Isolation and identification of fungi associated with spoilt fruits vended in Gwagwalada market, Abuja, Nigeria. Vet. World, 10(4), 393-397.
  • Hafez, E.E., Elbestawy, E. (2008). Molecular Chracterization of Soil Microorganisms: effect of industrial pollution on distribution and biodiversity. World J Microbiol Biotechnol. 25, 215-224.
  • Bardgett, R.D. (2005). The biology of soil: a community and ecosystem approach. Oxford University Press Inc, New York.
  • Hawksworth, D.L. (2001). The magnitude of fungal diversity: the 1.5 million species Estimate. Mycol. Res., 105(12), 1422-1432.
  • Abdullah, Q., Mahmoud, A., Al-harethi, A., (2016). Isolation and Identification of Fungal Post-harvest Rot of Some Fruits in Yemen. PSM Microbiol., 1(1), 36-44.
  • Jensen, B., Knudsen, I.M., Andersen, B., Nielsen, K.F., Thrane, U., Jensen, D.F., & Larsen, J. (2013). Characterization of microbial communities and fungal metabolites on field grown strawberries from organic and conventional production. Int. J. Food microbiol., 160(3), 313-322.
  • Samson, R.A., Hoekstra, E.S. and Frisvad, J.C. (2004). Introduction to Food-and Airborne Fungi, Laboratory Manual Series 2, Food and Indoor Fungi, 389pp.
  • Doyle J.J., Doyle J.L. (1987). Isolation of Plant DNA From Fresh Tissue. Focus, 12, 13-15.
  • Boysen M., Skoube P., Frisvad, J., Rossen, L. (1996). Reclassification of the Penicillium roqueforti group into three species on the basis of molecular genetic and biochemical profiles. Microbiol., 142, 541-519.
  • Baiyewu, R.A., Amusa, N.A., Ayoola, O.A., & Babalola, O.O. (2007). Survey of the postharvest diseases and aflatoxin contamination of marketed Pawpaw fruit (Carica papaya L.) in South Western Nigeria. Afr. J. Agric. Res., 2(4), 178-181.
  • Chukwuka, K.S., Okonko, I.O., & Adekunle, A.A. (2010). Microbial ecology of organisms causing pawpaw (Carica papaya L.) fruit decay in Oyo State, Nigeria. Am. Eurasian J. Toxicol. Sci., 2(1), 43-50.
  • Staats, M., Baarlen, P.V., Kan, J.A.V. (2004). Molecular Phylogeny of the Plant Pathogenic Genus Botrytis and the Evolution of Host Specificity. Mol. Bio. Evol., 22(2), 333-346.
  • Kasiamdari, R.S., Smith, E.S., Scott, E.S., Smith, F.A. (2002). Identification of binucleate Rhizoctonia as a contaminant in pot cultures of arbuscular mycorrhizal fungi and development of a PCR-based method of detection. Mycol. Res. 106 (12), 1417-1426.
  • Khairnar, D.N., Kelhe, A.S., Khairnar, A.B. (2011). Soil-borne Fungal Biodiversity of Some Fruit Crops of Nashik District and Control Measures. Nat. Envir. Pollution Tech., 10(1), 127-128.
  • Abdelfattah, A, Li Destri Nicosia, M.G, Cacciola S.O., Droby, S, Schena, L. (2015). Metabarcoding Analysis of Fungal Diversity in the Phyllosphere and Carposphere of Olive (Olea europaea). PLoS ONE, 10(7), e0131069.
  • Van Hemelrijck, W., Ceustermans, A., Van Campenhout, J., Lieten, P., Bylemans, D. (2017). Crown rot in strawberry caused by Pestalotiopsis. Acta Horticul., 1156, 781-786.
  • Zhao, J.N., Ma,, Z., Liu, Z.P. (2016). Pestalotiopsis clavispora causing leaf spot on strawberry. Mycosystema, 35(1), 114-120.
  • Adrian, M., Jeandet, P., Veneau, J., Weston, L.A., & Bessis, R. (1997). Biological activity of resveratrol, a stilbenic compound from grapevines, against Botrytis cinerea, the causal agent for gray mold. J. Chem. Eco., 23(7), 1689-1702.
  • Lagopodi, A.L., & Thanassoulopoulos, C.C. (1998). Effect of a leaf spot disease caused by Alternaria alternata on yield of sunflower in Greece. Plant Disease, 82(1), 41-44.
  • Johnson, G. I., Sangchote, S., & Cooke, A. W. (1990). Control of stem end rot (Dothiorella dominicana) and other postharvest diseases of mangoes (cv. Kensington Pride) during short-and long-term storage. Tropic. Agricult. Trinidad and Tobago, 67(2), 183-187.
  • Narasimhan, M.L., Damsz, B., Coca, M.A., Ibeas, J.I., Yun, D.J., Pardo, J.M., & Bressan, R.A. (2001). A plant defense response effector induces microbial apoptosis. Mol. Cell, 8(4), 921-930.
  • Menkis, A., Vasiliauskas, R., Taylor, A.F.S., Stenström, E., Stenlid, J., & Finlay, R. (2006). Fungi in decayed roots of conifer seedlings in forest nurseries, afforested clear‐cuts and abandoned farmland. Plant Patho., 55(1), 117-129.
  • Di Pietro, A., García‐Maceira, F. I., Meglecz, E., & Roncero, M.I.G. (2001). A MAP kinase of the vascular wilt fungus Fusarium oxysporum is essential for root penetration and pathogenesis. Mol. Microbiology, 39 (5), 1140-1152.

Ayrıntılar

Birincil Dil İngilizce
Konular Biyoloji
Yayınlanma Tarihi January
Bölüm Makaleler
Yazarlar

Bahadır TÖRÜN Bu kişi benim
Adnan Menderes University
https://orcid.org/0000-0001-5142-1882
Türkiye


Mehmet Ali YÖRÜKCE Bu kişi benim
Adnan Menderes University
https://orcid.org/0000-0003-1920-1921
Türkiye


Fatma YAMAN Bu kişi benim
Adnan Menderes University
https://orcid.org/0000-0002-4505-4091
Türkiye


Halil BIYIK
Adnan Menderes University
https://orcid.org/0000-0003-0258-054X
Türkiye

Yayımlanma Tarihi 4 Ocak 2018
Yayınlandığı Sayı Yıl 2018, Cilt 5, Sayı 1

Kaynak Göster

APA Törün, B. , Yörükce, M. A. , Yaman, F. & Bıyık, H. (2018). Biodiversity of Fungi in Strawberry Fields in Anamur, TURKEY . International Journal of Secondary Metabolite , 5 (1) , 20-26 . DOI: 10.21448/ijsm.346209
International Journal of Secondary Metabolite (IJSM)
ISSN-e: 2148-6905