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Fungal Biodiversity of Strawberry Fields in Aydin, TURKEY

Yıl 2018, Cilt 5, Sayı 2, 60 - 66, 05.07.2018
https://doi.org/10.21448/ijsm.398367

Öz

Strawberry is a kind of delicious and aromatic fruit, which can be consumed as fresh and also is suitable for industry. However, strawberry is exposed to many fungal diseases. The aim of this study is to determine the fungi that present in the field whether or not pathogenic. Samples were collected from different strawberry fields in Aydın in April 2015. Morphological identification was made according to the shape and color of the colonies, mycelium and spore structures. For molecular identification, ITS rDNA gene region was used. According to morphological and molecular methods, eleven different fungal genera were found on strawberries.

Kaynakça

  • Reddy, C.S., Ghai, R., Rashmi, K., & Kalai, V.C. (2003). Polyhydroxyalkanoates: an overview. Bioresource Technol., 87(2), p.137-146.
  • Singh, D., & Sharma, R.R. (2007). Postharvest diseases of fruit and vegetables and their management. In: Prasad, D., editor. Sustainable Pest Management. Daya Publishing House, New Delhi, India.
  • Al-Hindi, R.R., Al-Najada, A.R., & Mohamed, S.A. (2011). Isolation and identification of some fruit spoilage fungi: Screening of plant cell wall degrading enzymes. Afr. J. Microbiol. Res., 5(4), 443-448.
  • Droby, S. (2006). Improving quality and safety of fresh fruits and vegetables after harvest by the use of biocontrol agents and natural materials. Acta Hortic., 709, 45-51.
  • Mailafia S, Okoh GR, Olabode HOK, Osanupin R (2017) Isolation and identification of fungi associated with spoilt fruits vended in Gwagwalada market, Abuja, Nigeria, Veterinary World, 10(4), 393-397.
  • Bardgett, R.D. (2005). The biology of soil: a community and ecosystem approach. Oxford University Press Inc, New York.
  • Hawksworth, D. L. (2001). The magnitude of fungal diversity: the 1.5 million species estimate revisited** Paper presented at the Asian Mycological Congress 2000 (AMC 2000), incorporating the 2nd Asia-Pacific Mycological Congress on Biodiversity and Biotechnology, and held at the University of Hong Kong on 9-13 July 2000. Mycological research, 105(12), 1422-1432.
  • Samson, R.A., Hoekstra, E.S., & Frisvad, J.C. (2004). Introduction to Food-and Airborne Fungi, Laboratory Manual Series 2, Food and Indoor Fungi, 389pp.
  • Doyle, J.J., Doyle, J.L., (1987). Isolation of Plant DNA From Fresh Tissue, Focus, 12, 13-15.
  • Boysen, M., Skoube, P., Frisvad, J., Rossen, L. (1996). Reclassification of the Penicillium roqueforti group into three species on the basis of molecular genetic and biochemical profiles. Microbiology. 142, 541-519.
  • Baiyewu, R.A., Amusa, N.A., Ayoola, O.A. and Babalola, O.O. (2007). Survey of the postharvest diseases and aflatoxin contamination of marketed Pawpaw fruit (Carica papaya L.) in South Western Nigeria. Afr. J. Agric. Res., 2(4), 178-181.
  • Chukwuka, K.S., Okonko, I.O. and Adekunle, A.A. (2010). Microbial ecology of organisms causing pawpaw (Carica papaya L.) fruit decay in Oyo State, Nigeria. Am. Eurasian J. Toxicol. Sci., 2(1), 43-50.
  • Staats, M., Baarlen, P.V., Kan J.A.V. (2004). Molecular Phylogeny of the Plant Pathogenic Genus Botrytis and the Evolution of Host Specificity. Molecular Biology and Evolution, 22(2), 333-346.
  • Kasiamdari, R.S., Smith, E.S., Scott, E.S., Smith, F.A. (2002). Identification of binucleate Rhizoctonia as a contaminant in pot cultures of arbuscular mycorrhizal fungi and development of a PCR-based method of detection. Mycol. Res. 106 (12), 1417–1426.
  • Khairnar. D. N., Kelhe. A. S., Khairnar. A. B. (2011). Soil-borne Fungal Biodiversity of Some Fruit Crops of Nashik District and Control Measures. Nature Environment and Pollution Technology, 10(1), 127-128.
  • Adrian, M., Jeandet, P., Veneau, J., Weston, L. A., & Bessis, R. (1997). Biological activity of resveratrol, a stilbenic compound from grapevines, against Botrytis cinerea, the causal agent for gray mold. Journal of Chemical Ecology, 23(7), 1689-1702.
  • Bellanger, A. P., Reboux, G., Botterel, F., Candido, C., Roussel, S., Rognon, B., Millon, L. (2010). New evidence of the involvement of Lichtheimia corymbifera in farmer's lung disease. Medical mycology, 48(7), 981-987.
  • Gautam, R., Singh, S. K., & Sharma, V. (2016). Molecular diagnosis and intraspecific genetic variability of root pathogens of arid legumes in Western Rajasthan, India. Revista de Biología Tropical, 64(4).
  • Howell, C. R. (2003). Mechanisms employed by Trichoderma species in the biological control of plant diseases: the history and evolution of current concepts. Plant disease, 87(1), 4-10.
  • Lagopodi, A. L., & Thanassoulopoulos, C. C. (1998). Effect of a leaf spot disease caused by Alternaria alternata on yield of sunflower in Greece. Plant disease, 82(1), 41-44.

Fungal Biodiversity of Strawberry Fields in Aydin, TURKEY

Yıl 2018, Cilt 5, Sayı 2, 60 - 66, 05.07.2018
https://doi.org/10.21448/ijsm.398367

Öz

Strawberry is a kind of delicious and aromatic fruit, which can be consumed as fresh and also is suitable for industry. However, strawberry is exposed to many fungal diseases. The aim of this study is to determine the fungi that present in the field whether or not pathogenic. Samples were collected from different strawberry fields in Aydın in April 2015. Morphological identification was made according to the shape and color of the colonies, mycelium and spore structures. For molecular identification, ITS rDNA gene region was used. According to morphological and molecular methods, eleven different fungal genera were found on strawberries.

Kaynakça

  • Reddy, C.S., Ghai, R., Rashmi, K., & Kalai, V.C. (2003). Polyhydroxyalkanoates: an overview. Bioresource Technol., 87(2), p.137-146.
  • Singh, D., & Sharma, R.R. (2007). Postharvest diseases of fruit and vegetables and their management. In: Prasad, D., editor. Sustainable Pest Management. Daya Publishing House, New Delhi, India.
  • Al-Hindi, R.R., Al-Najada, A.R., & Mohamed, S.A. (2011). Isolation and identification of some fruit spoilage fungi: Screening of plant cell wall degrading enzymes. Afr. J. Microbiol. Res., 5(4), 443-448.
  • Droby, S. (2006). Improving quality and safety of fresh fruits and vegetables after harvest by the use of biocontrol agents and natural materials. Acta Hortic., 709, 45-51.
  • Mailafia S, Okoh GR, Olabode HOK, Osanupin R (2017) Isolation and identification of fungi associated with spoilt fruits vended in Gwagwalada market, Abuja, Nigeria, Veterinary World, 10(4), 393-397.
  • Bardgett, R.D. (2005). The biology of soil: a community and ecosystem approach. Oxford University Press Inc, New York.
  • Hawksworth, D. L. (2001). The magnitude of fungal diversity: the 1.5 million species estimate revisited** Paper presented at the Asian Mycological Congress 2000 (AMC 2000), incorporating the 2nd Asia-Pacific Mycological Congress on Biodiversity and Biotechnology, and held at the University of Hong Kong on 9-13 July 2000. Mycological research, 105(12), 1422-1432.
  • Samson, R.A., Hoekstra, E.S., & Frisvad, J.C. (2004). Introduction to Food-and Airborne Fungi, Laboratory Manual Series 2, Food and Indoor Fungi, 389pp.
  • Doyle, J.J., Doyle, J.L., (1987). Isolation of Plant DNA From Fresh Tissue, Focus, 12, 13-15.
  • Boysen, M., Skoube, P., Frisvad, J., Rossen, L. (1996). Reclassification of the Penicillium roqueforti group into three species on the basis of molecular genetic and biochemical profiles. Microbiology. 142, 541-519.
  • Baiyewu, R.A., Amusa, N.A., Ayoola, O.A. and Babalola, O.O. (2007). Survey of the postharvest diseases and aflatoxin contamination of marketed Pawpaw fruit (Carica papaya L.) in South Western Nigeria. Afr. J. Agric. Res., 2(4), 178-181.
  • Chukwuka, K.S., Okonko, I.O. and Adekunle, A.A. (2010). Microbial ecology of organisms causing pawpaw (Carica papaya L.) fruit decay in Oyo State, Nigeria. Am. Eurasian J. Toxicol. Sci., 2(1), 43-50.
  • Staats, M., Baarlen, P.V., Kan J.A.V. (2004). Molecular Phylogeny of the Plant Pathogenic Genus Botrytis and the Evolution of Host Specificity. Molecular Biology and Evolution, 22(2), 333-346.
  • Kasiamdari, R.S., Smith, E.S., Scott, E.S., Smith, F.A. (2002). Identification of binucleate Rhizoctonia as a contaminant in pot cultures of arbuscular mycorrhizal fungi and development of a PCR-based method of detection. Mycol. Res. 106 (12), 1417–1426.
  • Khairnar. D. N., Kelhe. A. S., Khairnar. A. B. (2011). Soil-borne Fungal Biodiversity of Some Fruit Crops of Nashik District and Control Measures. Nature Environment and Pollution Technology, 10(1), 127-128.
  • Adrian, M., Jeandet, P., Veneau, J., Weston, L. A., & Bessis, R. (1997). Biological activity of resveratrol, a stilbenic compound from grapevines, against Botrytis cinerea, the causal agent for gray mold. Journal of Chemical Ecology, 23(7), 1689-1702.
  • Bellanger, A. P., Reboux, G., Botterel, F., Candido, C., Roussel, S., Rognon, B., Millon, L. (2010). New evidence of the involvement of Lichtheimia corymbifera in farmer's lung disease. Medical mycology, 48(7), 981-987.
  • Gautam, R., Singh, S. K., & Sharma, V. (2016). Molecular diagnosis and intraspecific genetic variability of root pathogens of arid legumes in Western Rajasthan, India. Revista de Biología Tropical, 64(4).
  • Howell, C. R. (2003). Mechanisms employed by Trichoderma species in the biological control of plant diseases: the history and evolution of current concepts. Plant disease, 87(1), 4-10.
  • Lagopodi, A. L., & Thanassoulopoulos, C. C. (1998). Effect of a leaf spot disease caused by Alternaria alternata on yield of sunflower in Greece. Plant disease, 82(1), 41-44.

Ayrıntılar

Birincil Dil İngilizce
Konular Biyoloji
Yayınlanma Tarihi 07-07-2018
Bölüm Makaleler
Yazarlar

H. Halil Bıyık (Sorumlu Yazar)
Adnan Menderes University
orcid.org/0000-0003-0258-054X
Türkiye


Zeynep Ün Bu kişi benim


Bahadır Törün Bu kişi benim
orcid.org/0000-0001-5142-1882


Esin Poyrazoğlu Bu kişi benim
orcid.org/0000-0002-3921-5362

Yayımlanma Tarihi 5 Temmuz 2018
Yayınlandığı Sayı Yıl 2018, Cilt 5, Sayı 2

Kaynak Göster

APA Bıyık, H. H. , Ün, Z. , Törün, B. & Poyrazoğlu, E. (2018). Fungal Biodiversity of Strawberry Fields in Aydin, TURKEY . International Journal of Secondary Metabolite , 5 (2) , 60-66 . DOI: 10.21448/ijsm.398367
International Journal of Secondary Metabolite (IJSM)
ISSN-e: 2148-6905