Analysis of Phytochemical Composition and Biological Activities of Verbascum cheiranthifolium var. cheiranthifolium stem and flowers

Yıl 2018, Cilt: 5 Sayı: 3, 233 - 242, 15.09.2018

Abdullah Dalar Aydin Sukru Bengu Oruc Allahverdiyev

https://doi.org/10.21448/ijsm.401127

Cited By: 3

Öz

Within this study phytochemical composition,
antioxidant and enzyme inhibitory activities of extracts obtained from stem and
flower of Verbascum cheiranthifolium var.
cheiranthifolium were analysed. Both
of the extracts were detected as rich sources of phenolics (verbascoside and
luteolin hexoside), various volatile and fatty acid compounds. Luteolin
hexoside rich stem extract had pronounced FCR, FRAP and α-glucosidase inhibitory
activities. Flower extract had high levels of ORAC assay and effectively
suppressed activity of pancreatic lipase enzyme, which was rich in verbascoside
compound. Phenolic compounds and volatile compounds present in the extracts
might be the main contributors of antioxidant capacity and enzyme inhibitory
activities of the stem and flower extracts. Pronounced
antioxidant and enzyme inhibitory activities and rich bioactive composition
determined in this study reveal that Verbascum
cheiranthifolium var. cheiranthifolum
extracts might be a good source for natural health attributing sources.

Anahtar Kelimeler

Antioxidant, α-Glucosidase, Pancreatic Lipase, Phenolics

Analysis of Phytochemical Composition and Biological Activities of Verbascum cheiranthifolium var. cheiranthifolium stem and flowers

Öz

Within this study phytochemical composition, antioxidant and enzyme inhibitory activities of extracts obtained from stem and flower of Verbascum cheiranthifolium var. cheiranthifolium were analysed. Both of the extracts were detected as rich sources of phenolics (verbascoside and luteolin hexoside), various volatile and fatty acid compounds. Luteolin hexoside rich stem extract had pronounced FCR, FRAP and α-glucosidase inhibitory activities. Flower extract had high levels of ORAC assay and effectively suppressed activity of pancreatic lipase enzyme, which was rich in verbascoside compound. Phenolic compounds and volatile compounds present in the extracts might be the main contributors of antioxidant capacity and enzyme inhibitory activities of the stem and flower extracts. Pronounced antioxidant and enzyme inhibitory activities and rich bioactive composition determined in this study reveal that Verbascum cheiranthifolium var. cheiranthifolum extracts might be a good source for natural health attributing sources.

Anahtar Kelimeler

Antioxidant, α-Glucosidase, Pancreatic Lipase, Phenolics, Volatiles

Kaynakça

• Tatlı, İ.İ., Akdemir, Z.Ş. (2004). Chemical constituents of Verbascum L. species, FABAD Journal of Pharmaceutical Sciences, 29, 93-107.
• Mükemre, M., Behçet, L., Çakılcıoğlu, U. (2015). Ethnobotanical study on medicinal plants in villages of Çatak (Van-Turkey), Journal of Ethnopharmacology, 166, 361-374.
• Dalar, A., Guo, Y., Konczak, I. (2014). Phenolic composition and potential anti inflammatory properties of Verbascum cheiranthifolium var. cheiranthifolium leaf, Journal of Herbal Medicine, 4(4), 195-200.
• Tatlı, İ.İ., Akdemir, Z.Ş. (2006). Traditional uses and biological activities of Verbascum species, FABAD Journal of Pharmaceutical Sciences, 31, 85-96.
• Küçük, S., Özdemir, F., İşcan, G., İncesu, Z. (2016) Determination of Cytotoxic and Anticandidal Activities of Three Verbascum L. Species from Turkey: V. cheiranthifolium Boiss. var. asperulum (Boiss.) Murb. Monorg., V. pycnostachyum Boiss. & Heldr and V. orgyale Boiss. & Heldr., Turkish Journal of Pharmaceutical Sciences, 13(3), 318-322.
• Dalar, A., Konczak, I. (2013). Phenolic contents, antioxidant capacities and inhibitory activities against key metabolic syndrome relevant enzymes of herbal teas from Eastern Anatolia, Industrial Crops and Products, 44, 383-390.
• Uzun, Y., Dalar, A., Konczak, I. (2017). Sempervivum davisii: phytochemical composition, antioxidant and lipase-inhibitory activities, Pharmaceutical Biology, 55(1), 532-540.
• Shahidi F., Zhong Y. (2015). Measurement of antioxidant activity, Journal of Functional Foods, 18, 757-781.
• Tadera, K., Minami, Y., Takamatsu, K., Matsuoka, T. (2006). Inhibition of α-glucosidase and α-amylase by flavonoids, Journal of Nutritional Science and Vitaminology, 52(2), 149-153.
• Aldini, G., Piccoli, A., Beretta, G., Morazzoni, P., Riva, A., Marinello, C., Facino, R.M. (2006). Antioxidant activity of polyphenols from solid olive residues of cv Coratina, Fitoterapia, 77(2), 121-128.
• Piné, R., Herranz-López, M., Funes, L., Borrás-Linares, I., Micol, V., Segura-Carretero, A., Fernández- Gutiérrez, A. (2013). Phenylpropanoids and their metabolites are the major compounds responsible for blood-cell protection against oxidative stress after administration of Lippia citriodora in rats, Phytomedicine, 20(12), 1112-1118.
• Wong, I.Y.F., He, Z.D., Huang, Y., Chen, Z.Y. (2001). Antioxidative activities of phenylethanoid glycosides from Ligustrum purpurascens, Journal of Agricultural and Food Chemistry, 49, 3113–3119.
• Chen, C.H., Lin, Y.S., Chien, M.Y., Hou, W.C., Hu, M.L. (2012). Antioxidant and antihypertensive activities of acteoside and its analogs. Botanical Studies, 53, 421–429.
• Koo, K.A., Kim, S.H., Oh, T.H., Kim, Y.C. (2006). Acteoside and its aglycones protect primary cultures of rat cortical cells from glutamate-induced excitotoxicity. Life Sciences, 79, 709–716.
• Manju, V., Balasubramaniyan, V., Nalini, N. (2005). Rat colonic lipid peroxidation and antioxidant status: the effect of dietary luteolin on 1, 2-dimethylhydrazine challenge, Cellular and Molecular Biology Letters, 10, 535-551.
• Chen, C.Y., Peng, W.H., Tsai, K.D., Hsu, S.L. (2007). Luteolin supresses inflammation-associated gene expression by blocking NF-kappaB and AP-1 activation pathway in mouse alveolar macrophages, Life Sciences, 81, 1602-1614.
• López-Lázaro, M. (2009). Distribution and Biological Activities of the Flavonoid Luteolin, Mini Reviews in Medicinal Chemistry, 9, 31-59.
• Ruberto, J., Baratta, M.T. (2000). Antioxidant activity of selected essential oil components in two lipid model systems, Food Chemistry, 69, 167−74.