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Agrobacterium – caused transformation of cultivars Amaranthus caudatus L. and hybrids of A. caudatus L. x A. paniculatus L.

Yıl 2018, Cilt: 5 Sayı: 4, 312 - 318, 29.12.2018
https://doi.org/10.21448/ijsm.478267

Öz

The procedure for vacuum infiltration of cultivars A. caudatus L. and hybrids of A. caudatus L. xA. paniculatus L. was optimized. The functioning of gene construction pCBv19 in the Amaranthus leaves was evaluated by the transient expression after vacuum infiltration with Agrobacterium rhizogenes A4. After hypocotyl transformation of the varieties of amaranth species A. caudatus L.: Helios, Karmin, Kremovyi rannii, and hybrids A. caudatus x A. paniculatus L. – cv. Sterkh, A. caudatus x Sterkh- cv. Zhaivir with the wild strain A. rhizogenes A4, the culture of "hairy roots" was obtained. Embedding and transcription of genes in the roots are confirmed by the results of the PCR analysis.

Kaynakça

  • [1]. Biswas, M., Das, S.S., Dey, S. (2013). Establishment of a stable Amaranthus tricolor callus line for production of food colorant. Food Sci. Biotechnol, 22, 22 - 30.
  • [2]. Yaacob, J.S., Hwei, L.C., Taha, R.M. (2012). Pigment analysis and tissue culture of Amaranthus cruentus L., Acta horticulturae, 54 – 64.
  • [3]. Draper, J., Scott, R. (1991). The isolation of plant nucleic acids. In Plant Genetic Engineering Edited by Don Garierson. Blackie, London, pp. 240–244.
  • [4]. De Cleene, M., De Ley, J. (1976). The host range of Crown Gall. Bot. Rev. 42, 389–466.
  • [5]. Swain, S.S., Sahu, L., Barik, D.P., Chand, P.K. (2010). Agrobacterium×plant factors influencing transformation of “Joseph’s coat” (Amaranthus tricolor L.). Scientia Horticulturae, 125, 461-468.
  • [6]. Pal, A., Swain, S. S., Mukherjee, A. K. & Chand, P.K. (2013). Agrobacterium pRi TL-DNA rolB and TR-DNA Opine Genes Transferred to the Spiny Amaranth (Amaranthus spinosus L.) – A Nutraceutical Crop. Food technology and Biotechnology, 51 (1), 26–35. [7]. Swain S.S., Sahu L., Barik D.P., Chand P.K. (2009). Genetic transformation of Amaranthus tricolor L. using Ri plasmid vectors. In: Bastia, A.K. and Mohapatra, U.B. (eds.) Recent trends in monitoring and bioremediation of mine and industrial environment. Proc. Natl. Sem., North Orissa University, Orissa, 109-116.
  • [8] Pal, A., Swain, S.S., Das, A.B., Mukherjee, A.K., Chand, P.K. (2013). Stable germ line transformation of a leafy vegetable crop amaranth (Amaranthus tricolor L.) mediated by Agrobacterium tumefaciens, In Vitro Cell. Dev. Biol.—Plant, 114 - 128. https://link.springer.com/article/10.1007/s11627-013-9489-9 doi: 10.1007/s11627-013-9489-9
  • [9]. Taipova R.М., Kuluev B.R. (2015) Amaranth features of culture, prospects of cultivation in Russia and generation of transgenic Russian varieties, Biomica, v. 7, 4, 284-299.
  • [10]. Martins, P.K., Nakayama, T.J., Ribeiro, A.P., Cunha Badbd, Nepomuceno, A.L., Harmon, F.G., Kobayashi, A.K., Molinari, H.B. (2015) Setaria viridis floral-dip: A simple and rapid Agrobacterium-mediated transformation method, Biotechnol. Rep. (Amst.). https://www.ncbi.nlm.nih.gov/pubmed/28435809 doi:10.1016/j.btre.2015.02.006
  • [11]. Jefferson, R.A. (1987). Assaying chimeric genes in plants: the gus gene fusion system, Plant. Mol. Biol. Rep., 5, 387–405.
  • [12]. Murashige, T., Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures, Physiologia Plantarum, 15, 473-497.
  • [13]. Jofre-Garfias, A.E., Villegas-Sepúlveda, Cabrera-Ponce J.L., Adam e-Alvarez R.M., Herrera-Estrella, L., Simpson J. (1997). Agrobacterium mediated transformation of Amaranthus hypochondriacus: light- and tissue-specific expression of a pea chlorophyll a/b-binding protein promoter, Plant Cell Reports, 16, 847-852.
  • [14]. Stewart, C.N., Via, L.E. (1993) A Rapid CTAB DNA isolation technique useful for Rapid fingerprinting and other PCR applications, BioTechniques, v. 14 (5), 748-749.
  • [15]. Umaiyal Munusamy, Siti Nor Akmar Abdullah, Maheran Abd Aziz, Huzwah Khazaai (2013). Female reproductive system of Amaranthus as the target for Agrobacterium-mediated transformation, Advances in Bioscience and Biotechnology, 4, 188-192. http://dx.doi.org/10.4236/abb.2013.42027

Agrobacterium – caused transformation of cultivars Amaranthus caudatus L. and hybrids of A. caudatus L. x A. paniculatus L.

Yıl 2018, Cilt: 5 Sayı: 4, 312 - 318, 29.12.2018
https://doi.org/10.21448/ijsm.478267

Öz

The procedure for vacuum infiltration of cultivars
A. caudatus
L. and hybrids of A. caudatus
L. xA. paniculatus L. was
optimized.
The functioning of
gene construction pCBv19 in the Amaranthus
leaves was evaluated by the transient expression after vacuum infiltration with
Agrobacterium rhizogenes A4. After hypocotyl transformation of the varieties
of amaranth species  A. caudatus L.: Helios, Karmin, Kremovyi rannii, and hybrids A. caudatus x A. paniculatus L. – cv. Sterkh,
A. caudatus x Sterkh- cv. Zhaivir with the wild strain A. rhizogenes A4, the culture of "hairy roots" was obtained. Embedding and transcription of genes in the
roots are confirmed by the results of the PCR analysis.

Kaynakça

  • [1]. Biswas, M., Das, S.S., Dey, S. (2013). Establishment of a stable Amaranthus tricolor callus line for production of food colorant. Food Sci. Biotechnol, 22, 22 - 30.
  • [2]. Yaacob, J.S., Hwei, L.C., Taha, R.M. (2012). Pigment analysis and tissue culture of Amaranthus cruentus L., Acta horticulturae, 54 – 64.
  • [3]. Draper, J., Scott, R. (1991). The isolation of plant nucleic acids. In Plant Genetic Engineering Edited by Don Garierson. Blackie, London, pp. 240–244.
  • [4]. De Cleene, M., De Ley, J. (1976). The host range of Crown Gall. Bot. Rev. 42, 389–466.
  • [5]. Swain, S.S., Sahu, L., Barik, D.P., Chand, P.K. (2010). Agrobacterium×plant factors influencing transformation of “Joseph’s coat” (Amaranthus tricolor L.). Scientia Horticulturae, 125, 461-468.
  • [6]. Pal, A., Swain, S. S., Mukherjee, A. K. & Chand, P.K. (2013). Agrobacterium pRi TL-DNA rolB and TR-DNA Opine Genes Transferred to the Spiny Amaranth (Amaranthus spinosus L.) – A Nutraceutical Crop. Food technology and Biotechnology, 51 (1), 26–35. [7]. Swain S.S., Sahu L., Barik D.P., Chand P.K. (2009). Genetic transformation of Amaranthus tricolor L. using Ri plasmid vectors. In: Bastia, A.K. and Mohapatra, U.B. (eds.) Recent trends in monitoring and bioremediation of mine and industrial environment. Proc. Natl. Sem., North Orissa University, Orissa, 109-116.
  • [8] Pal, A., Swain, S.S., Das, A.B., Mukherjee, A.K., Chand, P.K. (2013). Stable germ line transformation of a leafy vegetable crop amaranth (Amaranthus tricolor L.) mediated by Agrobacterium tumefaciens, In Vitro Cell. Dev. Biol.—Plant, 114 - 128. https://link.springer.com/article/10.1007/s11627-013-9489-9 doi: 10.1007/s11627-013-9489-9
  • [9]. Taipova R.М., Kuluev B.R. (2015) Amaranth features of culture, prospects of cultivation in Russia and generation of transgenic Russian varieties, Biomica, v. 7, 4, 284-299.
  • [10]. Martins, P.K., Nakayama, T.J., Ribeiro, A.P., Cunha Badbd, Nepomuceno, A.L., Harmon, F.G., Kobayashi, A.K., Molinari, H.B. (2015) Setaria viridis floral-dip: A simple and rapid Agrobacterium-mediated transformation method, Biotechnol. Rep. (Amst.). https://www.ncbi.nlm.nih.gov/pubmed/28435809 doi:10.1016/j.btre.2015.02.006
  • [11]. Jefferson, R.A. (1987). Assaying chimeric genes in plants: the gus gene fusion system, Plant. Mol. Biol. Rep., 5, 387–405.
  • [12]. Murashige, T., Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures, Physiologia Plantarum, 15, 473-497.
  • [13]. Jofre-Garfias, A.E., Villegas-Sepúlveda, Cabrera-Ponce J.L., Adam e-Alvarez R.M., Herrera-Estrella, L., Simpson J. (1997). Agrobacterium mediated transformation of Amaranthus hypochondriacus: light- and tissue-specific expression of a pea chlorophyll a/b-binding protein promoter, Plant Cell Reports, 16, 847-852.
  • [14]. Stewart, C.N., Via, L.E. (1993) A Rapid CTAB DNA isolation technique useful for Rapid fingerprinting and other PCR applications, BioTechniques, v. 14 (5), 748-749.
  • [15]. Umaiyal Munusamy, Siti Nor Akmar Abdullah, Maheran Abd Aziz, Huzwah Khazaai (2013). Female reproductive system of Amaranthus as the target for Agrobacterium-mediated transformation, Advances in Bioscience and Biotechnology, 4, 188-192. http://dx.doi.org/10.4236/abb.2013.42027
Toplam 14 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Yapısal Biyoloji
Bölüm Makaleler
Yazarlar

Olha Mikolaivna Yaroshko Bu kişi benim 0000-0003-2517-4472

Mykola Vyktorovych Kuchuk Bu kişi benim

Yayımlanma Tarihi 29 Aralık 2018
Gönderilme Tarihi 18 Haziran 2018
Yayımlandığı Sayı Yıl 2018 Cilt: 5 Sayı: 4

Kaynak Göster

APA Yaroshko, O. M., & Kuchuk, M. V. (2018). Agrobacterium – caused transformation of cultivars Amaranthus caudatus L. and hybrids of A. caudatus L. x A. paniculatus L. International Journal of Secondary Metabolite, 5(4), 312-318. https://doi.org/10.21448/ijsm.478267

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International Journal of Secondary Metabolite
e-ISSN: 2148-6905