Promoting effect of foliar silicon on steviol glycoside contents of Stevia rebaudiana Bertoni under salt stress

Yıl 2019, Cilt: 6 Sayı: 3, 263 - 269, 15.10.2019

İlkay Yavaş Fatih Mehmet Yılmaz Aydın Ünay

https://doi.org/10.21448/ijsm.547380

Cited By: 2

Öz

This study investigated
the effect of foliar silicon application on fresh weight and dry matter
contents as well as steviol glycoside contents of stevia (Stevia rebaudiana
Bertoni) grown under salt stress. In this respect, an experimental research
with factorial arrangement on a Completely Randomized Design with four
replications was conducted in plastic pots under greenhouse conditions. Four
different salinity levels (0, 25, 50, and 75 mM NaCl) were used for three weeks
old seedling and two different foliar silicone concentrations (12.5 and 25 mM)
were applied in the form of sodium silicate which was launched after 4 weeks of
planting. Both NaCl concentrations and foliar silicone application had
statistically significant effect (p≤0.05)
on fresh weight and dry matter characteristics and also on rebaudioside-A and
stevioside contents of stevia leaves which were harvested before flowering
period. Results showed that salt stress decreased both rebaudioside-A and
stevioside contents, but foliar silicone application improve negative
influences of NaCl treatments with even increasing the steviol glycoside
contents. Rebaudioside-A content did not change, but stevioside content
decreased with foliar silicone application in control conditions. However,
highest rebaudioside-A and stevioside accumulation were recorded in 25 mM
foliar silicone application under 50 mM NaCl stress. In these conditions,
rebaudioside-A and stevioside contents were 112 and 26% higher than control,
respectively. It could be concluded that the application of silicon
significantly enhanced the plants ability to withstand salt stress conditions
through increased silicon content, increased steviol glycoside production.
Silicon application could therefore improve crop production under salt stress.

Anahtar Kelimeler

NaCl stress, Rebaudioside-A, silicon, Stevia

Promoting effect of foliar silicon on steviol glycoside contents of Stevia rebaudiana Bertoni under salt stress

Öz

This study investigated the effect of foliar silicon application on fresh weight and dry matter contents as well as steviol glycoside contents of stevia (Stevia rebaudiana Bertoni) grown under salt stress. In this respect, an experimental research with factorial arrangement on a Completely Randomized Design with four replications was conducted in plastic pots under greenhouse conditions. Four different salinity levels (0, 25, 50, and 75 mM NaCl) were used for three weeks old seedling and two different foliar silicone concentrations (12.5 and 25 mM) were applied in the form of sodium silicate which was launched after 4 weeks of planting. Both NaCl concentrations and foliar silicone application had statistically significant effect (p≤0.05) on fresh weight and dry matter characteristics and also on rebaudioside-A and stevioside contents of stevia leaves which were harvested before flowering period. Results showed that salt stress decreased both rebaudioside-A and stevioside contents, but foliar silicone application improve negative influences of NaCl treatments with even increasing the steviol glycoside contents. Rebaudioside-A content did not change, but stevioside content decreased with foliar silicone application in control conditions. However, highest rebaudioside-A and stevioside accumulation were recorded in 25 mM foliar silicone application under 50 mM NaCl stress. In these conditions, rebaudioside-A and stevioside contents were 112 and 26% higher than control, respectively. It could be concluded that the application of silicon significantly enhanced the plants ability to withstand salt stress conditions through increased silicon content, increased steviol glycoside production. Silicon application could therefore improve crop production under salt stress.

Anahtar Kelimeler

NaCl stress, Rebaudioside-A, Silicon, Stevia, Stevioside

Kaynakça

• [1]. Zaman, M.M., Chowdhury, M.A.H., Chowdhury, T. 2015. Growth parameters and leaf biomass yield of stevia (Stevia rebaudiana Bertoni) as influenced by different soil types of Bangladesh. Journal of the Bangladesh Agricultural University, 13 (1), 31-37.
• [2]. Ahmed, S.A., Ibrahim, A.K., Gouda, M.S., Mohamed, W.W., Khattab, S. (2016). Diatomite alleviates the adverse effects of salinity stress on growth and yield of Stevia rebaudiana. International Journal of Advanced Biotechnology and Research, 7 (1), 10-21.
• [3]. Benhmimou, A., Ibriz, M., Al Faïz, C., Douaik, A., Khiraoui, A., Amchra, F.Z., Lage, M. (2017). Productivity of new sweet plant in Morocco (Stevia rebaudiana Bertoni) under water stress. Journal of Medicinal Plants Studies, 5(5), 126-131.
• [4]. Hamad, A.M. (2015). Productivity of Stevia Rebaudiana Bertoni Plant and its Affective by Salinity, Nitrogen and Potassium Fertilization. J. Plant Production, Mansoura Univ., 6 (9), 1543 – 1552.
• [5]. Pandey, M., Chikara, S.K. 2015. Effect of salinity and drought stress on growth parameters, glycoside content and expression level of vital genes in the steviol glycosides biosynthesis pathway of Stevia rebaudiana (Bertoni). Int. J. Genet., 7 (1), 153-160.
• [6]. Helmy, B.F., Abdelsalam, N.R., Ghonema, M.A., Khalid, A.E. 2016. Morphologgical Changes and Antioxidant Activity of Stevia rebaudiana under Salt Stress. Alexandria Science Exchange Journal, 37 (2), 326-336.
• [7]. Shahverdi, M.A., Omidi, H., Tabatabaei, S.J. (2017). Stevia (Stevia rebaudiana Bertoni) responses to NaCl stress: Growth, photosynthetic pigments, diterpene glycosides and ion content in root and shoot. Journal of the Saudi Society of Agricultural Sciences, 18 (4), 355-360. Doi.org/10.1016/j.jssas.2017.12.001
• [8]. Rachmawati, D., Sudjino, Asiyah, I.J. 2017. Response and Stevioside Levels of Stevıa (Stevia rebaudiana Bertoni) Grown At Different Light Intensity and Water Availability. Pak. J. Bot., 49(5), 1689-1695.
• [9]. Reis, M., Coelho, L., Santos, G., Kienle, U., Beltrão, J. (2015). Yield response of stevia (Stevia rebaudiana Bertoni) to the salinity of irrigation water. Agri Water Manag., 152, 217-221.
• [10]. Rezende, R.A.L.S., Rodrigues, F.A., Soares, J.D.R., de Oliveira Silveira, H.R., Pasqual, M., de Maria Gomes Dias, G. (2018). Salt stress and exogenous silicon influence physiological and anatomical features of in vitro-grown cape gooseberry. Ciência Rural., 48 (1), 1-9.
• [11]. Abbaspour, H., Afshari, H., Abdel-Wahhab, A. (2012). Influence of salt stress on growth, pigments, soluble sugars and ion accumulation in three pistachio cultivars. J. Med. Plants Res., 6 (12), 2468-2473.
• [12]. Ziv, M. (2010). Silicon effects on growth acclimatization and stress tolerance of bioreactor cultured Ornıthogalum dubıum plants. Acta Hortic., 865, 29-35.
• [13]. Kumar Tripathi, D., Pratap Singh, V., Ahmad, P., Kumar Chauhan, D., Mohan Prasad, S. (2016). Silicon in Plants. Advances and Future Prospects. 1st Edition. CRC Press. 392 pages
• [14]. Abdulameer, D.A., Osman, M.B., Sulaiman, Z., Yusop, M.R., Abdullah, S., Azizi, P., Muttaleb, Q.A. (2018). Assessment of Stevia rebaudiana Bertoni Genotypes via Morpho Agronomic Traits under Two Light Conditions. American Journal of Plant Sciences, 9 (7),1403-1413.
• [15]. Jentzer, J.B., Alignan, M., Vaca-Garcia, C., Rigal, L., Vilarem, G. (2015). Response surface methodology to optimise Accelerated Solvent Extraction of steviol glycosides from Stevia rebaudiana Bertoni leaves. Food chemistry, 166, 561-567.
• [16]. SPSS Inc. (1999). SPSS for Windows: Base 10.0 Applications Guide. Chicago, Illinois
• [17]. Cantabella, D., Piqueras, A., Acosta-Motos, J.R., Bernal-Vicente, A., Hernandez, J.A., 210 Díaz-Vivancos, P. (2017). Salt tolerance mechanisms induced in Stevia rebaudiana Bertoni: Effects on mineral nutrition, antioxidative metabolism and steviol glycoside content. Plant Physiology and Biochemistry, 115, 484-496.
• [18]. Fallah, F., Nokhasi, F., Ghaheri, M., Kahrizi, D., Beheshti Ale, A.A., Ghorbani, T., Kazemi, E., Ansarypour, Z. (2017). Effect of salinity on gene expression, morphological and biochemical characteristics of Stevia rebaudiana Bertoni under in vitro conditions. Cell Mol Biol (Noisy-le-grand). 63(7), 102-106. doi: 10.14715/cmb/2017.63.7.17
• [19]. Zeng, J., Chen, A., Li, D., Yi, B., Wu, W. 2013. Effects of salt stress on the growth, physiological responses, and glycoside contents of Stevia rebaudiana Bertoni. J. Agric. Food Chem., 61, 5720-5726.
• [20]. El-Housini, E., Ahmed, M.A., Hassanein, M.S., Tawfik, M.M. (2014). Effect of Salicylic Acid (SA) on Growth and Quality of Stevia (Stevia rebaudiana Bert.) Under Salt Stress. American-Eurasian J. Agric. & Environ. Sci., 14 (4), 275-281.
• [21]. Noori Akandi, Z., Pirdashti, H., Yaghoubian, Y., Ghasemi Omran, V. (2017). Quantifying the response of growth and physiological parameters of stevia (Stevia rebaudiana Bertoni) medicinal plant to salinity stress under controlled conditions. J. Sci. & Technol. Greenhouse Culture, 8(1), 10-19.