Antioxidant activity of polyphenol compounds extracted from Nypa fruticans Wurmb. (Nipa palm) fruit husk with different ethanol concentration
Year 2024,
Volume: 11 Issue: 2, 355 - 363, 03.06.2024
Sabri Sudirman
,
Aprilia Kusuma Wardana
Herpandi Herpandı
Indah Widiastuti
Dwi Inda Sarı
Miftahul Janna
Abstract
Oxidative stress is a condition characterized by a higher content of free radicals than the potential antioxidants in the body. Exogenous antioxidants are needed to resolve this condition. The Nypa fruticans (Nipa palm) fruit husk is a source of polyphenol potential and can be used as a natural antioxidant agent. Therefore, this study aimed to determine the effect of ethanol concentration on polyphenol and tannin contents and their antioxidant activities. The polyphenol substances were extracted using several ethanol concentrations, whereas the antioxidant activity was determined using the 2,2-diphenyl-1-picrylhydrazyl method. The results show that the ethanol concentration has no effect on the yield of extraction. However, it affects the total polyphenol and tannin contents with high levels in the 50% and 70% ethanol concentrations. Fifty percent ethanol exhibits more effective antioxidant activity when compared to other ethanol concentrations. Therefore, a 50% ethanol concentration is a suitable solvent to extract polyphenol and tannin substances from nipa palm fruit husk and can be used as an alternative natural antioxidant.
Supporting Institution
DIPA of Public service Agency of Universitas Sriwijaya
Project Number
SP DIPA-023.17.2.677515/2023, on November 30, 2022, and 0188/UN9.3.1/SK/2023, on April 18, 2023
References
- Buamard, N., & Benjakul, S. (2017). Ethanolic coconut husk extract: In vitro antioxidative activity and effect on oxidative stability of shrimp oil emulsion. European Journal of Lipid Science and Technology, 119(11). https://doi.org/10.1002/ejlt.201700131
- Chandra, S., Khan, S., Avula, B., Lata, H., Yang, M.H., Elsohly, M.A., & Khan, I.A. (2014). Assessment of total phenolic and flavonoid content, antioxidant properties, and yield of aeroponically and conventionally grown leafy vegetables and fruit crops: a comparative study. Evidence-Based Complementary and Alternative Medicine, 2014, 253875. https://doi.org/10.1155/2014/253875
- Chemat, Abert, V., Ravi, Khadhraoui, Hilali, Perino, & Tixier. (2019). Review of alternative solvents for green extraction of food and natural products: Panorama, principles, applications and prospects. Molecules, 24(16). https://doi.org/10.3390/molecules24163007
- Chew, K.K., Ng, S.Y., Thoo, Y.Y., Khoo, M.Z., Wan Aida, W.M., & Ho, C.W. (2011). Effect of ethanol concentration, extraction time and extraction temperature on the recovery of phenolic compounds and antioxidant capacity of Centella asiatica extracts. International Food Research Journal, 18, 571-578.
- Do, Q.D., Angkawijaya, A.E., Tran-Nguyen, P.L., Huynh, L.H., Soetaredjo, F.E., Ismadji, S., & Ju, Y.-H. (2014). Effect of extraction solvent on total phenol content, total flavonoid content, and antioxidant activity of Limnophila aromatica. Journal of Food and Drug Analysis, 22(3), 296-302. https://doi.org/10.1016/j.jfda.2013.11.001
- Gazali, M., Nufus, H., Nurjanah, N., & Zuriat, Z. (2019). The Exploration potency of bioactive compounds of leaves extract nipah (Nypa fruticans Wurmb) from the coast of west aceh as antioxidant. Jurnal Pengolahan Hasil Perikanan Indonesia, 22(1). https://doi.org/10.17844/jphpi.v22i1.25892
- Goutzourelas, N., Kevrekidis, D.P., Barda, S., Malea, P., Trachana, V., Savvidi, S., Kevrekidou, A., Assimopoulou, A.N., Goutas, A., Liu, M., Lin, X., Kollatos, N., Amoutzias, G.D., & Stagos, D. (2023). Antioxidant activity and inhibition of liver cancer cells’ growth of extracts from 14 marine macroalgae species of the Mediterranean Sea. Foods, 12(6). https://doi.org/10.3390/foods12061310
- Haq, S.H., Al-Ruwaished, G., Al-Mutlaq, M.A., Naji, S.A., Al-Mogren, M., Al-Rashed, S., Ain, Q.T., Al-Amro, A.A., & Al-Mussallam, A. (2019). Antioxidant, anticancer activity and phytochemical analysis of green algae, chaetomorpha collected from the Arabian Gulf. Scientific Reports, 9(1). https://doi.org/10.1038/s41598-019-55309-1
- Herpandi, Lestari, S.D., Bastian, & Sudirman, S. (2021). Antioxidant activity of the fractions from water lettuce (Pistia stratiotes) extract. Food Research, 5(2), 451-455. https://doi.org/10.26656/fr.2017.5(2).578
- Hikmawanti, E.N.P., Fatmawati, S., & Asri, A.W. (2021). The effect of ethanol concentrations as the extraction solvent on antioxidant activity of katuk (Sauropus androgynus (L.) Merr.) Leaves Extracts. IOP Conference Series: Earth and Environmental Science, 755(1). https://doi.org/10.1088/1755-1315/755/1/012060
- Ismail, M.M., El Zokm, G.M., & Miranda Lopez, J.M. (2023). Nutritional, bioactive compounds content, and antioxidant activity of brown seaweeds from the Red Sea. Frontiers in Nutrition, 10. https://doi.org/10.3389/fnut.2023.1210934
- Jakfar, & Azwar. (2023). Optimization of tannin extraction from areca nuts (Areca cateshu Linn) using ethanol solvent. Jurnal Serambi Engineering, 8(1), 4917-4924.
- Jitrangsri, K., Chaidedgumjorn, A., & Satiraphan, M. (2020). Effect of ethanol percentage upon various extraction methods of peanut based on antioxidant activity with trans-resveratrol and total phenolic contents. Pharmaceutical Sciences Asia, 47(2), 164 172. https://doi.org/10.29090/psa.2020.02.018.0056
- Lee, C.Y., Nanah, C.N., Held, R.A., Clark, A.R., Huynh, U.G.T., Maraskine, M.C., Uzarski, R.L., McCracken, J., & Sharma, A. (2015). Effect of electron donating groups on polyphenol based antioxidant dendrimers. Biochimie, 111, 125 134. https://doi.org/10.1016/j.biochi.2015.02.001
- Lezoul, N.E.H., Belkadi, M., Habibi, F., & Guillén, F. (2020). Extraction Processes with Several Solvents on Total Bioactive Compounds in Different Organs of Three Medicinal Plants. Molecules, 25(20). https://doi.org/10.3390/molecules25204672
- Lobo, V., Patil, A., Phatak, A., & Chandra, N. (2010). Free radicals, antioxidants and functional foods: Impact on human health. Pharmacognosy Reviews, 4(8), 118 126. https://doi.org/10.4103/0973-7847.70902
- Lourenço, S.C., Moldão-Martins, M., & Alves, V.D. (2019). Antioxidants of natural plant origins: From sources to food industry applications. Molecules, 24(22). https://doi.org/10.3390/molecules24224132
- Maisetta, G., Batoni, G., Caboni, P., Esin, S., Rinaldi, A.C., & Zucca, P. (2019). Tannin profile, antioxidant properties, and antimicrobial activity of extracts from two Mediterranean species of parasitic plant Cytinus. BMC Complementary and Alternative Medicine, 19(1). https://doi.org/10.1186/s12906-019-2487-7
- Mojzer, E.B., Hrnčič, M.K., Škerget, M., Knez, Ž., & Bren, U. (2016). Polyphenols: Extraction Methods, Antioxidative Action, Bioavailability and Anticarcinogenic Effects. Molecules, 21(7). https://doi.org/10.3390/molecules21070901
- Moreno, N.J., Volpe, F., Moler, J.A., Esparza, I., & Ancín-Azpilicueta, C. (2019). Impact of Extraction Conditions on the Phenolic Composition and Antioxidant Capacity of Grape Stem Extracts. Antioxidants, 8(12). https://doi.org/10.3390/antiox8120597
- Plaskova, A., & Mlcek, J. (2023). New insights of the application of water or ethanol-water plant extract rich in active compounds in food. Frontiers in Nutrition, 10. https://doi.org/10.3389/fnut.2023.1118761
- Prasad, N., Yang, B., Kong, K.W., Khoo, H.E., Sun, J., Azlan, A., Ismail, A., & Romli, Z.B. (2013). Phytochemicals and Antioxidant Capacity fromNypa fruticansWurmb. Fruit. Evidence Based Complementary and Alternative Medicine, 2013, 1 9. https://doi.org/10.1155/2013/154606
- Rad, M.S., Anil Kumar, N.V., Zucca, P., Varoni, E.M., Dini, L., Panzarini, E., Rajkovic, J., Tsouh Fokou, P.V., Azzini, E., Peluso, I., Prakash Mishra, A., Nigam, M., El Rayess, Y., Beyrouthy, M.E., Polito, L., Iriti, M., Martins, N., Martorell, M., Docea, A.O., Setzer, W.N., Calina, D., Cho, W.C., & Sharifi-Rad, J. (2020). Lifestyle, oxidative stress, and antioxidants: back and forth in the pathophysiology of chronic diseases. Frontiers in Physiology, 11. https://doi.org/10.3389/fphys.2020.00694
- Rajkumar, G., Panambara, P.A.H.R., & Sanmugarajah, V. (2022). Comparative analysis of qualitative and quantitative phytochemical evaluation of selected leaves of medicinal plants in Jaffna, Sri Lanka. Borneo Journal of Pharmacy, 5(2), 93 103. https://doi.org/10.33084/bjop.v5i2.3091
- Safithri, M., Nur Faridah, D., Ramadani, F., & Pratama, R. (2022). Antioxidant activity of ethanol extract and fractions of Piper crocatum with Rancimat and cuprac methods. Turkish Journal of Biochemistry, 47(6), 795-801. https://doi.org/10.1515/tjb-2021-0300
- Serang, Y., & Laili, V. (2021). Measuring the antioxidant effect of limnocharis flava on malondialdehyde activities in livers of alloxan-induced diabetic rats. Jurnal Info Kesehatan, 19(2), 181-186. https://doi.org/10.31965/infokes.Vol19.Iss2.534
- Sudirman, S., Herpandi, Safitri, E., Apriani, E.F., & Taqwa, F.H. (2022). Total polyphenol and flavonoid contents and antioxidant activities of water lettuce (Pistia stratiotes) leave extracts. Food Research, 6(4), 205-210. https://doi.org/10.26656/fr.2017.6(4).484
- Sulaiman, I.S.C., Basri, M., Fard Masoumi, H.R., Chee, W.J., Ashari, S.E., & Ismail, M. (2017). Effects of temperature, time, and solvent ratio on the extraction of phenolic compounds and the anti-radical activity of Clinacanthus nutans Lindau leaves by response surface methodology. Chemistry Central Journal, 11(1). https://doi.org/10.1186/s13065-017-0285-1
- Sun, C., Wu, Z., Wang, Z., & Zhang, H. (2015). Effect of ethanol/water solvents on phenolic profiles and antioxidant properties of beijing propolis extracts. Evidence-Based Complementary and Alternative Medicine, 2015, 1-9. https://doi.org/10.1155/2015/595393
- Thoo, Y.Y., Ng, S.Y., Khoo, M.Z., Aida, W.W.M., & Ho, C.W. (2013). A binary solvent extraction system for phenolic antioxidants and its application to the estimation of antioxidant capacity in Andrographis paniculata extracts. International Food Research Journal, 20(3), 1103-1111.
- Xu, D.-P., Li, Y., Meng, X., Zhou, T., Zhou, Y., Zheng, J., Zhang, J.-J., & Li, H.-B. (2017). Natural antioxidants in foods and medicinal plants: extraction, assessment and resources. International Journal of Molecular Sciences, 18(1). https://doi.org/10.3390/ijms18010096
- Zhang, Z.-S., Li, D., Wang, L.-J., Ozkan, N., Chen, X.D., Mao, Z.-H., & Yang, H.-Z. (2007). Optimization of ethanol–water extraction of lignans from flaxseed. Separation and Purification Technology, 57(1), 17-24. https://doi.org/10.1016/j.seppur.2007.03.006
- Złotek, U., Mikulska, S., Nagajek, M., & Świeca, M. (2016). The effect of different solvents and number of extraction steps on the polyphenol content and antioxidant capacity of basil leaves (Ocimum basilicum L.) extracts. Saudi Journal of Biological Sciences, 23(5), 628-633. https://doi.org/10.1016/j.sjbs.2015.08.002
Antioxidant activity of polyphenol compounds extracted from Nypa fruticans Wurmb. (Nipa palm) fruit husk with different ethanol concentration
Year 2024,
Volume: 11 Issue: 2, 355 - 363, 03.06.2024
Sabri Sudirman
,
Aprilia Kusuma Wardana
Herpandi Herpandı
Indah Widiastuti
Dwi Inda Sarı
Miftahul Janna
Abstract
Oxidative stress is a condition characterized by a higher content of free radicals than the potential antioxidants in the body. Exogenous antioxidants are needed to resolve this condition. The Nypa fruticans (Nipa palm) fruit husk is a source of polyphenol potential and can be used as a natural antioxidant agent. Therefore, this study aimed to determine the effect of ethanol concentration on polyphenol and tannin contents and their antioxidant activities. The polyphenol substances were extracted using several ethanol concentrations, whereas the antioxidant activity was determined using the 2,2-diphenyl-1-picrylhydrazyl method. The results show that the ethanol concentration has no effect on the yield of extraction. However, it affects the total polyphenol and tannin contents with high levels in the 50% and 70% ethanol concentrations. Fifty percent ethanol exhibits more effective antioxidant activity when compared to other ethanol concentrations. Therefore, a 50% ethanol concentration is a suitable solvent to extract polyphenol and tannin substances from nipa palm fruit husk and can be used as an alternative natural antioxidant.
Project Number
SP DIPA-023.17.2.677515/2023, on November 30, 2022, and 0188/UN9.3.1/SK/2023, on April 18, 2023
References
- Buamard, N., & Benjakul, S. (2017). Ethanolic coconut husk extract: In vitro antioxidative activity and effect on oxidative stability of shrimp oil emulsion. European Journal of Lipid Science and Technology, 119(11). https://doi.org/10.1002/ejlt.201700131
- Chandra, S., Khan, S., Avula, B., Lata, H., Yang, M.H., Elsohly, M.A., & Khan, I.A. (2014). Assessment of total phenolic and flavonoid content, antioxidant properties, and yield of aeroponically and conventionally grown leafy vegetables and fruit crops: a comparative study. Evidence-Based Complementary and Alternative Medicine, 2014, 253875. https://doi.org/10.1155/2014/253875
- Chemat, Abert, V., Ravi, Khadhraoui, Hilali, Perino, & Tixier. (2019). Review of alternative solvents for green extraction of food and natural products: Panorama, principles, applications and prospects. Molecules, 24(16). https://doi.org/10.3390/molecules24163007
- Chew, K.K., Ng, S.Y., Thoo, Y.Y., Khoo, M.Z., Wan Aida, W.M., & Ho, C.W. (2011). Effect of ethanol concentration, extraction time and extraction temperature on the recovery of phenolic compounds and antioxidant capacity of Centella asiatica extracts. International Food Research Journal, 18, 571-578.
- Do, Q.D., Angkawijaya, A.E., Tran-Nguyen, P.L., Huynh, L.H., Soetaredjo, F.E., Ismadji, S., & Ju, Y.-H. (2014). Effect of extraction solvent on total phenol content, total flavonoid content, and antioxidant activity of Limnophila aromatica. Journal of Food and Drug Analysis, 22(3), 296-302. https://doi.org/10.1016/j.jfda.2013.11.001
- Gazali, M., Nufus, H., Nurjanah, N., & Zuriat, Z. (2019). The Exploration potency of bioactive compounds of leaves extract nipah (Nypa fruticans Wurmb) from the coast of west aceh as antioxidant. Jurnal Pengolahan Hasil Perikanan Indonesia, 22(1). https://doi.org/10.17844/jphpi.v22i1.25892
- Goutzourelas, N., Kevrekidis, D.P., Barda, S., Malea, P., Trachana, V., Savvidi, S., Kevrekidou, A., Assimopoulou, A.N., Goutas, A., Liu, M., Lin, X., Kollatos, N., Amoutzias, G.D., & Stagos, D. (2023). Antioxidant activity and inhibition of liver cancer cells’ growth of extracts from 14 marine macroalgae species of the Mediterranean Sea. Foods, 12(6). https://doi.org/10.3390/foods12061310
- Haq, S.H., Al-Ruwaished, G., Al-Mutlaq, M.A., Naji, S.A., Al-Mogren, M., Al-Rashed, S., Ain, Q.T., Al-Amro, A.A., & Al-Mussallam, A. (2019). Antioxidant, anticancer activity and phytochemical analysis of green algae, chaetomorpha collected from the Arabian Gulf. Scientific Reports, 9(1). https://doi.org/10.1038/s41598-019-55309-1
- Herpandi, Lestari, S.D., Bastian, & Sudirman, S. (2021). Antioxidant activity of the fractions from water lettuce (Pistia stratiotes) extract. Food Research, 5(2), 451-455. https://doi.org/10.26656/fr.2017.5(2).578
- Hikmawanti, E.N.P., Fatmawati, S., & Asri, A.W. (2021). The effect of ethanol concentrations as the extraction solvent on antioxidant activity of katuk (Sauropus androgynus (L.) Merr.) Leaves Extracts. IOP Conference Series: Earth and Environmental Science, 755(1). https://doi.org/10.1088/1755-1315/755/1/012060
- Ismail, M.M., El Zokm, G.M., & Miranda Lopez, J.M. (2023). Nutritional, bioactive compounds content, and antioxidant activity of brown seaweeds from the Red Sea. Frontiers in Nutrition, 10. https://doi.org/10.3389/fnut.2023.1210934
- Jakfar, & Azwar. (2023). Optimization of tannin extraction from areca nuts (Areca cateshu Linn) using ethanol solvent. Jurnal Serambi Engineering, 8(1), 4917-4924.
- Jitrangsri, K., Chaidedgumjorn, A., & Satiraphan, M. (2020). Effect of ethanol percentage upon various extraction methods of peanut based on antioxidant activity with trans-resveratrol and total phenolic contents. Pharmaceutical Sciences Asia, 47(2), 164 172. https://doi.org/10.29090/psa.2020.02.018.0056
- Lee, C.Y., Nanah, C.N., Held, R.A., Clark, A.R., Huynh, U.G.T., Maraskine, M.C., Uzarski, R.L., McCracken, J., & Sharma, A. (2015). Effect of electron donating groups on polyphenol based antioxidant dendrimers. Biochimie, 111, 125 134. https://doi.org/10.1016/j.biochi.2015.02.001
- Lezoul, N.E.H., Belkadi, M., Habibi, F., & Guillén, F. (2020). Extraction Processes with Several Solvents on Total Bioactive Compounds in Different Organs of Three Medicinal Plants. Molecules, 25(20). https://doi.org/10.3390/molecules25204672
- Lobo, V., Patil, A., Phatak, A., & Chandra, N. (2010). Free radicals, antioxidants and functional foods: Impact on human health. Pharmacognosy Reviews, 4(8), 118 126. https://doi.org/10.4103/0973-7847.70902
- Lourenço, S.C., Moldão-Martins, M., & Alves, V.D. (2019). Antioxidants of natural plant origins: From sources to food industry applications. Molecules, 24(22). https://doi.org/10.3390/molecules24224132
- Maisetta, G., Batoni, G., Caboni, P., Esin, S., Rinaldi, A.C., & Zucca, P. (2019). Tannin profile, antioxidant properties, and antimicrobial activity of extracts from two Mediterranean species of parasitic plant Cytinus. BMC Complementary and Alternative Medicine, 19(1). https://doi.org/10.1186/s12906-019-2487-7
- Mojzer, E.B., Hrnčič, M.K., Škerget, M., Knez, Ž., & Bren, U. (2016). Polyphenols: Extraction Methods, Antioxidative Action, Bioavailability and Anticarcinogenic Effects. Molecules, 21(7). https://doi.org/10.3390/molecules21070901
- Moreno, N.J., Volpe, F., Moler, J.A., Esparza, I., & Ancín-Azpilicueta, C. (2019). Impact of Extraction Conditions on the Phenolic Composition and Antioxidant Capacity of Grape Stem Extracts. Antioxidants, 8(12). https://doi.org/10.3390/antiox8120597
- Plaskova, A., & Mlcek, J. (2023). New insights of the application of water or ethanol-water plant extract rich in active compounds in food. Frontiers in Nutrition, 10. https://doi.org/10.3389/fnut.2023.1118761
- Prasad, N., Yang, B., Kong, K.W., Khoo, H.E., Sun, J., Azlan, A., Ismail, A., & Romli, Z.B. (2013). Phytochemicals and Antioxidant Capacity fromNypa fruticansWurmb. Fruit. Evidence Based Complementary and Alternative Medicine, 2013, 1 9. https://doi.org/10.1155/2013/154606
- Rad, M.S., Anil Kumar, N.V., Zucca, P., Varoni, E.M., Dini, L., Panzarini, E., Rajkovic, J., Tsouh Fokou, P.V., Azzini, E., Peluso, I., Prakash Mishra, A., Nigam, M., El Rayess, Y., Beyrouthy, M.E., Polito, L., Iriti, M., Martins, N., Martorell, M., Docea, A.O., Setzer, W.N., Calina, D., Cho, W.C., & Sharifi-Rad, J. (2020). Lifestyle, oxidative stress, and antioxidants: back and forth in the pathophysiology of chronic diseases. Frontiers in Physiology, 11. https://doi.org/10.3389/fphys.2020.00694
- Rajkumar, G., Panambara, P.A.H.R., & Sanmugarajah, V. (2022). Comparative analysis of qualitative and quantitative phytochemical evaluation of selected leaves of medicinal plants in Jaffna, Sri Lanka. Borneo Journal of Pharmacy, 5(2), 93 103. https://doi.org/10.33084/bjop.v5i2.3091
- Safithri, M., Nur Faridah, D., Ramadani, F., & Pratama, R. (2022). Antioxidant activity of ethanol extract and fractions of Piper crocatum with Rancimat and cuprac methods. Turkish Journal of Biochemistry, 47(6), 795-801. https://doi.org/10.1515/tjb-2021-0300
- Serang, Y., & Laili, V. (2021). Measuring the antioxidant effect of limnocharis flava on malondialdehyde activities in livers of alloxan-induced diabetic rats. Jurnal Info Kesehatan, 19(2), 181-186. https://doi.org/10.31965/infokes.Vol19.Iss2.534
- Sudirman, S., Herpandi, Safitri, E., Apriani, E.F., & Taqwa, F.H. (2022). Total polyphenol and flavonoid contents and antioxidant activities of water lettuce (Pistia stratiotes) leave extracts. Food Research, 6(4), 205-210. https://doi.org/10.26656/fr.2017.6(4).484
- Sulaiman, I.S.C., Basri, M., Fard Masoumi, H.R., Chee, W.J., Ashari, S.E., & Ismail, M. (2017). Effects of temperature, time, and solvent ratio on the extraction of phenolic compounds and the anti-radical activity of Clinacanthus nutans Lindau leaves by response surface methodology. Chemistry Central Journal, 11(1). https://doi.org/10.1186/s13065-017-0285-1
- Sun, C., Wu, Z., Wang, Z., & Zhang, H. (2015). Effect of ethanol/water solvents on phenolic profiles and antioxidant properties of beijing propolis extracts. Evidence-Based Complementary and Alternative Medicine, 2015, 1-9. https://doi.org/10.1155/2015/595393
- Thoo, Y.Y., Ng, S.Y., Khoo, M.Z., Aida, W.W.M., & Ho, C.W. (2013). A binary solvent extraction system for phenolic antioxidants and its application to the estimation of antioxidant capacity in Andrographis paniculata extracts. International Food Research Journal, 20(3), 1103-1111.
- Xu, D.-P., Li, Y., Meng, X., Zhou, T., Zhou, Y., Zheng, J., Zhang, J.-J., & Li, H.-B. (2017). Natural antioxidants in foods and medicinal plants: extraction, assessment and resources. International Journal of Molecular Sciences, 18(1). https://doi.org/10.3390/ijms18010096
- Zhang, Z.-S., Li, D., Wang, L.-J., Ozkan, N., Chen, X.D., Mao, Z.-H., & Yang, H.-Z. (2007). Optimization of ethanol–water extraction of lignans from flaxseed. Separation and Purification Technology, 57(1), 17-24. https://doi.org/10.1016/j.seppur.2007.03.006
- Złotek, U., Mikulska, S., Nagajek, M., & Świeca, M. (2016). The effect of different solvents and number of extraction steps on the polyphenol content and antioxidant capacity of basil leaves (Ocimum basilicum L.) extracts. Saudi Journal of Biological Sciences, 23(5), 628-633. https://doi.org/10.1016/j.sjbs.2015.08.002