Bacteria isolated from wound cultures and their antibiotic resistance status: an evaluation between 2019-2023

Year 2025, Volume: 16 Issue: 56, 155 - 161, 16.12.2025

Ahmet Şahin , Büşra Demir , Selda Aslan , Halim Bayram

https://doi.org/10.17944/interdiscip.1642578

Abstract

Objective: Determining the bacterial flora and antibiotic resistance profile of the hospital contributes to the infection management of clinicians during treatment period. The aim of this study is to evaluate the microorganisms isolated from wound specimens in our hospital and their antimicrobial resistance profiles.
Method: Patients who received inpatient treatment between 2019 and 2023 and had growth in wound cultures were included in our study. Wound swabs were cultured on 5% sheep blood agar, chocolate agar, and eosin methylene blue agar media. After incubation, the identification and antibiotic susceptibilities of the colonies were analyzed with a fully automated VITEK 2 system (bioMerieux, France). Antimicrobial profiles of the isolates were determined as susceptible or resistant. Data were evaluated retrospectively.
Results: A total of 462 patient samples with wound cultures were included in this study. Of the causative microorganisms identified, 361 (78.1%) were Gram-negative pathogens. The most frequently isolated bacteria were Proteus mirabilis (21.2%), Acinetobacter baumannii (A. baumannii) (16.7%), and Escherichia coli (E. coli) (13.9%). Staphylococcus aureus (S. aureus) was the most common Gram-positive bacteria. Meropenem resistance in E. coli, Klebsiella pneumoniae (K. pneumoniae), Pseudomonas aeruginosa, A. baumannii, and P. mirabilis was 6.2%, 17.4%, 11.4%, 44.2%, and 6.1%, respectively. The highest carbapenem resistance rates were observed in A. baumannii and K. pneumoniae, respectively.
Conclusion: Determination of the distribution of pathogenic microorganisms and antimicrobial resistance profiles in wound infections will contribute to the reduction of resistance rates by guiding the empirical treatment of clinicians.

Keywords

Wound culture , antibiotic , resistance , bacteria

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