The role of prostaglandin E(1) on the model of acute ischemic reperfusion-induced renal injury in rats

Yıl 2016, Cilt: 75 Sayı: 2, 7 - 17, 22.08.2017

Nurcan Orhan Şehnaz Bolkent

Öz

Prostaglandin E(1) is a natural prostaglandin that has various pharmacological effects. It has been shown
that prostaglandin E(1) has a protective action on some organs of rats treated with renal ischemia/
reperfusion. Our aim was to investigate the role of prostaglandin E(1) in rats with renal ischemia-
reperfusion-induced acute renal injury. Histological, immunohistochemical, and biochemical analyses
were performed. Sprague Dawley male rats were divided into four groups in this study. The first group
was given physiological saline only. Second group was administered prostaglandin E(1) (20 μg/kg) only.
Third group was treated with ischemia-reperfusion. Fourth group was administered prostaglandin E(1)
(20 μg/kg) and applied ischemia-reperfusion. All the rats were sacrificed after the reperfusion period.
Dissected kidney tissue was used for histological examination and biochemical analysis. The kidneys
of the experimental group with ischemia-reperfusion model have shown histopathologic changes as
an increase in proliferating cell nuclear antigen (PCNA) and caspase 3 immunoreactivity, a significant
decrease in reduced glutathione and DNA levels, and a significant increase in lipid peroxidation levels.
In contrast, the administration of prostaglandin E(1) reversed these effects on kidneys of the animals
applied ischemia-reperfusion model. As a summary, the histological, immunohistochemical, and
biochemical evaluations have revealed that prostaglandin E(1) ameliorated renal damage of rats with
renal ischemia/reperfusion. 

Anahtar Kelimeler

Acute ischemia-reperfusion, apoptosis, proliferation, prostaglandin E(1), rat

Kaynakça

• Beutler E. (1979) Glutathione in red cell metabolism a manual of biochemical methods. Second Edition, New York: Grune-Stratton: 112-4.
• Bonventre J.V. (2003) Dedifferentiation and proliferation of surviving epithelial cells in acute renal failure. Journal of the American Society of Nephrology, 14: S55-S61.
• Campos R., Maureira F., Garrido A. and Valenzuela A. (1993) Different glutathione redox status and lipid peroxidation in the cortex and the medulla of the rat kidney subjected to ischemia reperfusion stres. Comparative Biochemistry and Physiology, 105B: 157-63.
• Chatterjee K.P., Patel N.S.A., Cuzzocrea S., Brown P.A.J., Stewart K.N., Mota. lipe H., Britti D., Eberhardt W., Pfeilschifter J. and Thiemermann C. (2004) The cyclopentenone prostaglandin 15-deoxy-Δ12,14-prostaglandin J(2) ameliorates ischemic acute renal failure. Cardiovascular Research, 61: 30-643.
• Cuzzocrea S., Riley D.P., Caputi A.P. and Salvemini D. (2001) Antioxidant therapy: a new pharmacological approach in shock, in ammation and ischemia reperfusion injury. Pharmacological Review, 53: 135-59.
• Daemen M., Van’t Veer C., Denecker G., Heemskerk V.H., Wolfs T., Clauss M., Vandenabeele P. and Buurman W.A. (1999) Inhibition of apoptosis induced by ischemia reperfusion prevents in ammation. The Journal of Clinical Investigation, 104: 541-49.
• Eckle V.S., Buchmann A., Bursch W., Schulte- Hermann R. and Schwarz M. (2004) Immunohistochemical detection of activated caspases in apoptotic hepatocytes rat liver. Toxicologic Pathology, 32: 9-15.
• Freeman B.A. and Crapo J.D. (1982) Biology of disease, free radicals and tissue injury. Laboratory Investigation, 47: 412-26.
• Gezginci-Oktayoglu S, Orhan N, Bolkent S. (2016) Prostaglandin-E1 has a protective effect on renal ischemia/reperfusioninduced oxidative stress and in ammation mediated gastric damage in rats. Int Immunopharmacol., 36:142-50.
• Gobe G., Zhang X.J., Willgoss D.A., Schoch E., Hogg N.A. and Endre Z.H. (2000) Relationship between expression of Bcl- 2 genes and growth factors in ischemic acute renal failure in the rat. Journal of the American Society of Nehrology, 11: 454- 67.
• Gupta P.C., Matsushita M., Oda K., Nishikimi N., Sakurai T. and Nimura Y. (1998) Attenuation of renal ischemia-reperfusion injury in rats by allopurinol and prostaglandin E(1). European Surgical Research, 30: 102-7.
• Hauet T., Mothes D., Goujon J.M., Carretier M. and Eugene M. (2001) Protective effect of polyethylene glycol against prolonged cold ischemia and reperfusion injury: study in the isolated perfused rat kidney. The Journal of Pharmacology and Experimental Therapeutics, 297: 946-52.
• Hsieh CC, Hsieh SC, Chiu JH, Wu YL. (2014) Protective Effects of N-acetylcysteine and a Prostaglandin E1 Analog, Alprostadil, Against Hepatic Ischemia: Reperfusion Injury in Rats. J Tradit Complement Med., 4(1):64-71.
• Jo S.K., Yun S.Y., Chang K.H., Cha D.R., Cho W.Y., Kim H.K. and Won N.H. (2001) α-MSH decreases apoptosis in ischaemic acute renal failure in rats: possible mechanism of this bene cial effect. Nephrology Dialysis Transplantation, 16: 1583-91.
• Kaufman R.P., Kobzik L., Shepro D., Anner H., Valeri C.R. and Hechtman HB. (1987) Vasodilator prostaglandins (PG) prevent renal damage after ischemia. Annals of Surgery, 205: 195-98.
• Koch J.A., Plum J., Grabensee B. and Modder U. (2000). PGE(1) Study Group, Prostaglandin E(1): a new agent for the prevention of renal dysfunction in high risk patients caused by radiocontrast media. Nephrology Dialysis Transplantation, 15: 43-9.
• Kribben A., Edelstein C.L. and Schrier R.W. (1999) Pathophysiology of acute renal failure. Journal of Nephrology, 12: 142- 51.
• Kunduzova O.R., Bianchi P., Pizzinat N., Escourrou G., Sequelas M.H., Parini A. and Cambon C. (2002) Regulation of JNK/ ERK activation, cell apoptosis, and tissue regeneration by monoamine oxidases after renal ischemia reperfusion. The FASEB Journal, 15: 1129-31.
• Kunduzova O.R., Escourrou G., Seguelas M.H., Delagrence P., De La Farge F., Cambon C. and Parini A. (2003) Prevention of apoptotic and necrotic cell death, caspase-3 activation, and renal dysfunction by melatonin after ischemia/ reperfusion. The FASEB Journal, 17: 872- 74.
• Ledwozyw A., Michalak J., Stepien A. and Kadziolka A. (1986) The relationship between plasma triglycerides, cholesterol, total lipids and lipid peroxidation products during human atherosclerosis. Clin Chim Acta, 155: 275-83.
• Liu K.D. (2003) Molecular mechanisms of recovery from acute renal failure. Critical Care Medicine, 31: S572-S581. Lowry O.H., Rosebrough N.J., Farr A.L. and Randall R.J. (1951) Protein measurement with the Folin phenol reagent. J Biol Chem, 193: 265-75.
• Maulik N., Yoshida T. and Das D.K. (1998) Oxidative stress developed during the reperfusion of ischemic myocardium induces apoptosis. Free Radical Biology & Medicine, 24: 869-75.
• McCord J.M. (1985) Oxygen-derived free radicals in postischemic tissue injury. The New England Journal of Medicine, 312: 159-63.
• McDougal W.S. (1988) Renal perfusion/ reperfusion injuries. The Journal of Urology, 140: 1325-30. Mene P., Polci R. and Festuccia F. (2003) Mechanisms of repair after kidney injury. Journal of Nephrology, 16: 186-95.
• Nakajima T., Miyaji T., Kato A., Ikegaya N., Yamamato T. and Hishida A. (1996)Uninephrectomy reduces apoptotic cell death and enhances renal tubular cell regeneration in ischemic ARF in rats. American Journal of Physiology, 271: F846 F853.
• Nony P.A. and Schnellmann R.G. (2003) Mechanisms of renal cell repair and regeneration after acute renal failure. The Journal of Pharmacology and Experimental Therapeutics, 304: 905- 12.
• Numajiri Y., Taguchi Y., Koyama I., Sukigara M. and Omoto R. (1994) Protective effect of lipo-prostaglandin E(1) on warm ischemic kidney injury. Transplantation Proceedings, 26: 2397-2401.
• Oberbauer R., Schwarz C., Regele H.M., Hansmann C., Meyer T.W. and Mayer G. (2001) Regulation of renal tubular cell apoptosis and proliferation after ischemic injury to a solitary kidney. Journal of Laboratory and Clinical Medicine, 138: 343-51.
• Oztay F, Kara-Kisla B, Orhan N, Yanardag R, Bolkent S.The protective effects of prostaglandin E1 on lung injury following renal ischemia-reperfusion in rats. (2016) Toxicol Ind Health., 32(9):1684-92.
• Plummer D.T. (1978). The estimation of DNA by the dipenylamine reaction. In An introduction to practical biochemistry. New York: Mchraw-Hill: 215.
• Sehirli A.O., Sener G., Satiroglu H. and Gul A.D. (2003) Protective effect of N-acetylcysteine on renal ischemia reperfusion injury in the rat. Journal of Nephrology, 16: 75-80.
• Sener G., Sehirli A.O., Keyer-Uysal M., Arbak S., Ersoy Y. and Yegen B.C. (2002) The protective effect of melatonin on renal ischemia reperfusion injury in the rat. Journal of Pineal Research, 32: 120-26.
• Seth P., Kumari R., Madhavan S., Singh A.K., Mani H., Banaudha K.K., Sharma S.C., Kulshreshtha D.K. and Maheshwari R.K. (2000) Prevention of renal ischemia reperfusion-induced injury in rats by picroliv. Biochemical Pharmacology, 59: 1315- 22.
• Sheng R, Zhang LS, Han R, Gao B, Liu XQ, Qin ZH. (2011) Combined prostaglandin E1 and lithium exert potent neuroprotection in a rat model of cerebral ischemia. Acta Pharmacol Sin. 32(3):303-10.
• Shimizu A. and Yamanaka N. (1993) Apoptosis and cell desquamation in repair process of ischemic tubular necrosis. Virchows Archiv B Cell Pathology, 64: 171-80.
• Sung F.L., Zhu T.Y., AU-Yeung K.K.W., Siow Y.L. and Karmin O. (2002) Enhanced MCP-1 expression during ischemia/ reperfusion injury is mediated by oxidative stress and NF- KB. Kidney International, 62: 1160-70.
• Thiemermann C., Patel N.S.A., Kvale E.O., Cockerill G.W., Brown P.A.J., Stewart K.N., Cuzzocrea S., Britti D., Mota lipe H. and Chatterjee P.K. (2003) High density lipoorotein (HDL) reduces renal ischemia reperfusion injury. Journal of the American Society of Nehrology, 14: 1833-43.
• Tobimatsu M., Konomi K., Saito S. and Tsumagarı T. (1985) Protective effect of prostaglandin E(1) on ischemia–induced acute renal failure in dogs. Surgery, 98: 45-53.
• Ueda N., Kaushal G.P. and Shah S.V. (2000) Apoptotic mechanisms in acute renal failure. American Journal of Medicine, 108: 403-15.
• Ushigome H., Sano H., Okamoto M., Kadotani Y., Nakamura K., Akioka K., Yashimura R., Ohmori Y. and Yoshimura N. (2002) The role of tissue factor in renal ischemic reperfusion injury of the rat. Journal of Surgical Research, 102: 102-09.
• Wang S.S., Zhang T., Hong L. and Qi Q.H. (2003) Effect of arsenic trioxide on rat hepatocarcinoma and its renal cytotoxicity. World Journal of Gastroenterology, 9: 930-35.
• Weight S.C., Bell P.R.F. and Nicholson M.L. (1996) Renal ischaemia reperfusion injury. British Journal of Surgery, 83: 162-70.