Determination of the Distribution of the rs2069514 and rs762551 Alleles of the Cyp1a2 Gene Related to Caffeine Metabolism in Professional Athletes

Year 2017, Volume: 76 Issue: 2, 69 - 73, 27.12.2017

Begum Yucesoy Sezgin Kapici Canan Sercan Turkan Yigitbasi Nesrin Emekli Korkut Ulucan

https://doi.org/10.5152/EurJBiol.2017.1712

Abstract

The
objective of this study is to investigate allele distributions of the rs2069514
and rs762551 polymorphisms of the CYP1A2 gene which affect caffeine metabolism
in long-distance and short-distance runners in the Turkish population. 20
professional athletes in total were included in the study within the age range
of 18-24, 10 of whom were long-distance (female, n=5; Male, n=5) and 10 of whom
were short-distance (Female, n=5; Male, n=5) runners. DNA isolation from the
athletes was performed from peripheral blood samples and genotyping was
determined using the technique of Real Time-PCR. According to the obtained
data, the ratio of individuals having AA (n=6), GG (n=9) and AG (n=5) genotype
for the region of rs2069514 was found as 30%, 45% and 25% respectively. On the
other hand, the ratio for AA (n=4), CC (n=9) and AC (n=7) genotypes in the
region of rs762551 allele was determined as 20%, 45% and 35%. According to the
results, no significant variance regarding gender was found for either of the
different polymorphisms under study in long-distance and short-distance runners
while the genotype distributions varied between short and long-distance runners
only in the rs2069514 polymorphism. The GG genotype was observed in 9
short-distance runners while it was observed in none of the long-distance
runners. As a result, the fact that the GG genotype in case of the Rs2069514
polymorphism was observed in nine of the ten short-distance runners suggests
that the athletes in this group metabolize caffeine slowly and this should be
considered in the caffeine intake. 

Keywords

CYP1A2, caffeine, short-distance runner, long-distance runner, rs2069514, rs762551

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