LENTIL SEED ASPARTATE APvIINOTRANSFERASE ISOENZYMES I. ISOLATION and PARTIAL PURIFICATION

Yıl 1995, Cilt: 31 , 1 - 10, 09.09.2015

N. Akev*, A. Can* R. Yanardağ**

Öz

Three electrophoretically distinct aspartate minotransferase isoenzymes
named AAT-1, AAT-2and AAT-3, were separated from lentil (Lens culinaris
Medik.) seeds by extraction, ammonium sulphate precipitation, hydroxylapatite
and DEAE-cellulose column chromatographies. AAT-1 was purified 228,
AAT-2,42 and AAT-3, 3.8 fold. The isoenzymes were examined by means of
polyacrylamide gel electrophoresis.Owing to its key role in general metabolism, aspartate adotransferase (AAT, E.C. 2.6.1.1.) which was formerly called glutamic oxaloacetic transaminase (GOT), occurs widely in nature. In animals, cytbsolic and rnitochon+al forms of AAT have been characterized and purified (1-4). AAT has also been studied in various plants (5, 6) and different isoeozyrnic forms have been r&-, ported. The enzyme has been extensively characterized in wheat germ (7,8), cauliflower (9), cotton seeds (lo), pea (ll), soybean (12-14), oat (15), lupine (16) and alfalfa (17,18).

Anahtar Kelimeler

Lentil, Lens culinaris Medik., aspartate minotransferase, isoenzymes

LENTIL SEED ASPARTATE APvIINOTRANSFERASE ISOENZYMES I. ISOLATION and PARTIAL PURIFICATION

Öz

Elektroforetik balurndan far& ii~ aspartat aminotransferaz izoenzimi yegil mercimek (Lens culinaris Medik.) tohudarmdan ekstraksiyon , amonyum sulfatla goktiirme, hidroksilapatit ve DEAE-seluloz sutun kromatografisi yontemleri kullanllarak aynld~. AAT-1, AAT-2 ve AAT-3 olarak adlandlrilan bu izoenzimler, slras~yla 228, 42 ve 3.8 kez saflagtlrdd~. Izoenzimler poliakrilamid jel
elektroforezi ile incelendi.

Elektroforetik balurndan far& ii~ aspartat aminotransferaz izoenzimi yegil mercimek (Lens culinaris Medik.) tohudarmdan ekstraksiyon , amonyum sulfatla goktiirme, hidroksilapatit ve DEAE-seluloz sutun kromatografisi yontemleri kullanllarak aynld~. AAT-1, AAT-2 ve AAT-3 olarak adlandlrilan bu izoenzimler, slras~yla 228, 42 ve 3.8 kez saflagtlrdd~. Izoenzimler poliakrilamid jel
elektroforezi ile incelendi.

Anahtar Kelimeler

Lentil, Lens culinaris Medik, aspartate minotransferase, isoenzymes

Kaynakça

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