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LENTIL SEED ASPARTATE APvIINOTRANSFERASE ISOENZYMES I. ISOLATION and PARTIAL PURIFICATION

Year 1995, Volume: 31 , 1 - 10, 09.09.2015

N. Akev*, A. Can* R. Yanardağ**

Abstract

Three electrophoretically distinct aspartate minotransferase isoenzymes
named AAT-1, AAT-2and AAT-3, were separated from lentil (Lens culinaris
Medik.) seeds by extraction, ammonium sulphate precipitation, hydroxylapatite
and DEAE-cellulose column chromatographies. AAT-1 was purified 228,
AAT-2,42 and AAT-3, 3.8 fold. The isoenzymes were examined by means of
polyacrylamide gel electrophoresis.Owing to its key role in general metabolism, aspartate adotransferase (AAT, E.C. 2.6.1.1.) which was formerly called glutamic oxaloacetic transaminase (GOT), occurs widely in nature. In animals, cytbsolic and rnitochon+al forms of AAT have been characterized and purified (1-4). AAT has also been studied in various plants (5, 6) and different isoeozyrnic forms have been r&-, ported. The enzyme has been extensively characterized in wheat germ (7,8), cauliflower (9), cotton seeds (lo), pea (ll), soybean (12-14), oat (15), lupine (16) and alfalfa (17,18).

Keywords

Lentil Lens culinaris Medik. aspartate minotransferase isoenzymes

References

  • 1. Nisselbaum, J.S., Bodansky,O., J. Biol. Chem., 239,4232-4236 (1964).
  • 2. Nisselbaum, J.S., Bodansky, O., J. Biol. Clzenz., 241,2661-2664 (1966).
  • 3. Martinez-Carrion, M., Turano, C., Chiancone, E., et al., J. Biol. Chem., 242, 2397-2409 (1967).
  • 4. Shrawder, E.J., Martinez-Canion, M., J. Biol. Chenz. ,248,2140-2146 (1973).
  • 5. Givan, C.V., "The Biochemistry of Plants: A Comprehensive Treatise", pp. 329-357, Miflin, B.J. ed. Academic Press N.Y. (1980).-Ref: Turano, F.J., Wilson, B.J., Matthews, B.F., Plant Physiol., 92,587-594 (1990).
  • 6. Ireland, R.J., Joy, K.W., "Transaminases" pp. 376-383, Christen, P., Metzler, D.E. ed. John Wiley and Sons .N.Y. (1985).-Ref: Turano, F.J., Wilson, B.J., Matthews, B.F.. Plant Physiol., 92,587-594 (1990).
  • 7. Cruickshank, D.H., Isherwood, F.A., Biochenz. J., 69,189-195 (1958).
  • 8. Verjee, Z.H.M., Evered, D.F., Bioclzinl. Biopfzys. Acta, 185, 103-1 10 (1969).
  • 9. Ellis, R.J., Davies, D.D., Biochem. J., 78, 615-623 (1961).
  • 10. Fasella, P., Bossa. F., Turano, C., Rossi Fanelli, A., E~zqnzologia ,30, 198-205 (1966).
  • 11. Wong, K.F., Cossins, E.A., Plzytochemishy, 8, 1327-1338 (1969).
  • 12. Ryan, E., Bodley, F., Fottrell, P.F., Phytockemistry, 11,957-963 (1972).
  • 13. Oner, N., Can, A., Aydeger, N., Tiitem, E., Acta Pharmacezitica Tnrcica, 30,43-48 (1988).
  • 14. Oner, N., Aydeger, N., Can, A., Acta Pharnlacerctica Tzcrcica, 31.27-32 (1989).
  • 15. Reed, R.E., Hess, J.L., J.Bio1. Clzem., 250,4456-4461 (1975).
  • 16. Reynolds, P.H.S., Boland, M.J., Farnden, K.J.F., Arch. Biochenz. Biopyhs., 209,524-533 (1981).
  • 17. Griffith, S.M., Vance, C.P., Plarlt Plzysiol., 90, 1622-1629 (1989).
  • 18. Farnham, M.W., Miller, S.S., Griffith, S.M., Vance, C.P., Plarzt Pftysiol., 93, 603-610 (1990).
  • 19. Turano, F.J., Wilson, B.J., Matthews, B.F., Plarzt Plzysiol., 92, 587-594 (1990).
  • 20. Warburg, O., Christian, W., Biochenz. Z., 310,384-421 (1941).
  • 21. Karmen, A., J. Clitt. bzvest., 34, 131-133 (1955).
  • 22. Rej, R., Bretaudiere, J.-P., Graffunder, B., Clirz. Chern., 27,535-542 (1981).
  • 23. Frankel, S., :'Gradwohl's Clinical Laboratory Methods and Diagnosis", Vol 1, p. 126, Frankel, S., Reitman, S., Sonnenwirth, A.C. ed. The C.V. Mosby Company, Saint Louis (1970).
  • 24. Schwartz, M.K., Nisselbaum, J.S., Bodansky, O., Anz. J. Clirr. Patllol., 40, 103-106 (1963).
  • 25. Tiselius, A., HjertCn, S., Levin, O., Arch. Biocheni.Biopliys., 65, 132-155 (1956).

LENTIL SEED ASPARTATE APvIINOTRANSFERASE ISOENZYMES I. ISOLATION and PARTIAL PURIFICATION

Year 1995, Volume: 31 , 1 - 10, 09.09.2015

N. Akev*, A. Can* R. Yanardağ**

Abstract

Elektroforetik balurndan far& ii~ aspartat aminotransferaz izoenzimi yegil mercimek (Lens culinaris Medik.) tohudarmdan ekstraksiyon , amonyum sulfatla goktiirme, hidroksilapatit ve DEAE-seluloz sutun kromatografisi yontemleri kullanllarak aynld~. AAT-1, AAT-2 ve AAT-3 olarak adlandlrilan bu izoenzimler, slras~yla 228, 42 ve 3.8 kez saflagtlrdd~. Izoenzimler poliakrilamid jel
elektroforezi ile incelendi.

Elektroforetik balurndan far& ii~ aspartat aminotransferaz izoenzimi yegil mercimek (Lens culinaris Medik.) tohudarmdan ekstraksiyon , amonyum sulfatla goktiirme, hidroksilapatit ve DEAE-seluloz sutun kromatografisi yontemleri kullanllarak aynld~. AAT-1, AAT-2 ve AAT-3 olarak adlandlrilan bu izoenzimler, slras~yla 228, 42 ve 3.8 kez saflagtlrdd~. Izoenzimler poliakrilamid jel
elektroforezi ile incelendi.

Keywords

Lentil Lens culinaris Medik aspartate minotransferase isoenzymes

References

  • 1. Nisselbaum, J.S., Bodansky,O., J. Biol. Chem., 239,4232-4236 (1964).
  • 2. Nisselbaum, J.S., Bodansky, O., J. Biol. Clzenz., 241,2661-2664 (1966).
  • 3. Martinez-Carrion, M., Turano, C., Chiancone, E., et al., J. Biol. Chem., 242, 2397-2409 (1967).
  • 4. Shrawder, E.J., Martinez-Canion, M., J. Biol. Chenz. ,248,2140-2146 (1973).
  • 5. Givan, C.V., "The Biochemistry of Plants: A Comprehensive Treatise", pp. 329-357, Miflin, B.J. ed. Academic Press N.Y. (1980).-Ref: Turano, F.J., Wilson, B.J., Matthews, B.F., Plant Physiol., 92,587-594 (1990).
  • 6. Ireland, R.J., Joy, K.W., "Transaminases" pp. 376-383, Christen, P., Metzler, D.E. ed. John Wiley and Sons .N.Y. (1985).-Ref: Turano, F.J., Wilson, B.J., Matthews, B.F.. Plant Physiol., 92,587-594 (1990).
  • 7. Cruickshank, D.H., Isherwood, F.A., Biochenz. J., 69,189-195 (1958).
  • 8. Verjee, Z.H.M., Evered, D.F., Bioclzinl. Biopfzys. Acta, 185, 103-1 10 (1969).
  • 9. Ellis, R.J., Davies, D.D., Biochem. J., 78, 615-623 (1961).
  • 10. Fasella, P., Bossa. F., Turano, C., Rossi Fanelli, A., E~zqnzologia ,30, 198-205 (1966).
  • 11. Wong, K.F., Cossins, E.A., Plzytochemishy, 8, 1327-1338 (1969).
  • 12. Ryan, E., Bodley, F., Fottrell, P.F., Phytockemistry, 11,957-963 (1972).
  • 13. Oner, N., Can, A., Aydeger, N., Tiitem, E., Acta Pharmacezitica Tnrcica, 30,43-48 (1988).
  • 14. Oner, N., Aydeger, N., Can, A., Acta Pharnlacerctica Tzcrcica, 31.27-32 (1989).
  • 15. Reed, R.E., Hess, J.L., J.Bio1. Clzem., 250,4456-4461 (1975).
  • 16. Reynolds, P.H.S., Boland, M.J., Farnden, K.J.F., Arch. Biochenz. Biopyhs., 209,524-533 (1981).
  • 17. Griffith, S.M., Vance, C.P., Plarlt Plzysiol., 90, 1622-1629 (1989).
  • 18. Farnham, M.W., Miller, S.S., Griffith, S.M., Vance, C.P., Plarzt Pftysiol., 93, 603-610 (1990).
  • 19. Turano, F.J., Wilson, B.J., Matthews, B.F., Plarzt Plzysiol., 92, 587-594 (1990).
  • 20. Warburg, O., Christian, W., Biochenz. Z., 310,384-421 (1941).
  • 21. Karmen, A., J. Clitt. bzvest., 34, 131-133 (1955).
  • 22. Rej, R., Bretaudiere, J.-P., Graffunder, B., Clirz. Chern., 27,535-542 (1981).
  • 23. Frankel, S., :'Gradwohl's Clinical Laboratory Methods and Diagnosis", Vol 1, p. 126, Frankel, S., Reitman, S., Sonnenwirth, A.C. ed. The C.V. Mosby Company, Saint Louis (1970).
  • 24. Schwartz, M.K., Nisselbaum, J.S., Bodansky, O., Anz. J. Clirr. Patllol., 40, 103-106 (1963).
  • 25. Tiselius, A., HjertCn, S., Levin, O., Arch. Biocheni.Biopliys., 65, 132-155 (1956).
There are 25 citations in total.

Details

Primary Language English
Subjects Pharmacology and Pharmaceutical Sciences
Journal Section Makaleler
Authors

N. Akev*, A. Can* R. Yanardağ** This is me

Publication Date September 9, 2015
Published in Issue Year 1995 Volume: 31

Cite

APA R. Yanardağ**, N. A. A. C. (2015). LENTIL SEED ASPARTATE APvIINOTRANSFERASE ISOENZYMES I. ISOLATION and PARTIAL PURIFICATION. Journal of Faculty of Pharmacy of Istanbul University, 31, 1-10.
AMA R. Yanardağ** NAAC. LENTIL SEED ASPARTATE APvIINOTRANSFERASE ISOENZYMES I. ISOLATION and PARTIAL PURIFICATION. Journal of Faculty of Pharmacy of Istanbul University. September 2015;31:1-10.
Chicago R. Yanardağ**, N. Akev*, A. Can*. “LENTIL SEED ASPARTATE APvIINOTRANSFERASE ISOENZYMES I. ISOLATION and PARTIAL PURIFICATION”. Journal of Faculty of Pharmacy of Istanbul University 31, September (September 2015): 1-10.
EndNote R. Yanardağ** NAAC (September 1, 2015) LENTIL SEED ASPARTATE APvIINOTRANSFERASE ISOENZYMES I. ISOLATION and PARTIAL PURIFICATION. Journal of Faculty of Pharmacy of Istanbul University 31 1–10.
IEEE N. A. A. C. R. Yanardağ**, “LENTIL SEED ASPARTATE APvIINOTRANSFERASE ISOENZYMES I. ISOLATION and PARTIAL PURIFICATION”, Journal of Faculty of Pharmacy of Istanbul University, vol. 31, pp. 1–10, 2015.
ISNAD R. Yanardağ**, N. Akev*, A. Can*. “LENTIL SEED ASPARTATE APvIINOTRANSFERASE ISOENZYMES I. ISOLATION and PARTIAL PURIFICATION”. Journal of Faculty of Pharmacy of Istanbul University 31 (September 2015), 1-10.
JAMA R. Yanardağ** NAAC. LENTIL SEED ASPARTATE APvIINOTRANSFERASE ISOENZYMES I. ISOLATION and PARTIAL PURIFICATION. Journal of Faculty of Pharmacy of Istanbul University. 2015;31:1–10.
MLA R. Yanardağ**, N. Akev*, A. Can*. “LENTIL SEED ASPARTATE APvIINOTRANSFERASE ISOENZYMES I. ISOLATION and PARTIAL PURIFICATION”. Journal of Faculty of Pharmacy of Istanbul University, vol. 31, 2015, pp. 1-10.
Vancouver R. Yanardağ** NAAC. LENTIL SEED ASPARTATE APvIINOTRANSFERASE ISOENZYMES I. ISOLATION and PARTIAL PURIFICATION. Journal of Faculty of Pharmacy of Istanbul University. 2015;31:1-10.