Evaluation of anticancer effects of Aloe vera and aloe emodin on B16F10 murine melanoma and NIH3T3 mouse embryogenic fibroblast cells

Yıl 2017, Cilt: 47 Sayı: 3, 77 - 83, 11.10.2017

Eda Çandöken Serap Erdem Kuruca Nuriye Akev

https://doi.org/10.5152/IstanbulJPharm.2017.0013

Öz

Aloe vera (L.) Burm. f.
is well known for its beneficial effects on the skin. Moreover, the
antioxidant, immunostimulant, and anticancer effects of the plant leaf extracts
have been reported in scientific research. This study was conducted to
demonstrate the cytotoxic effects of several leaf extracts and aloe emodin (AE)
on a type of skin cancer. A. vera aqueous and methanolic extracts of fresh
leaves, methanolic extract of dried leaves, and leaf gel extract (AVG) were
prepared separately. Cytotoxicity was assessed using the MTT test. Apoptosis
and necrosis were detected by flow cytometry using Annexin V/PI. All the
extracts exhibited a selective cytotoxic effect on the cells. The mechanism of
AVG cytotoxicity on B16F10 murine melanoma cells was found to be apoptosis, whereas
that of AE was necrosis. The observation that treatment with AVG delayed the
apoptosis in NIH3T3 cells, while it exerted an apoptotic activity on B16F10
cells, provides some scientific evidence for the folkloric and alternative uses
of A. vera gel as a protective and skin healer. Therefore, A. vera gel and aloe
emodin can be used as potential targets for anticancer drug research. 

Anahtar Kelimeler

Aloe vera, Aloe gel, Aloe emodin, Melanoma, Cytotoxicity, Apoptosis

Kaynakça

• Referans 1: Prof. Dr. Ayşe CAN [e-mail]
• Referans 2: Prof. Dr. Uzay Görmüş [e-mail]