Effect of gel formulation obtained from Fomes fomentarius on bleeding and clotting time: A pilot study

Yıl 2020, Cilt: 50 Sayı: 3, 216 - 223, 30.12.2020

Gülşah Gedik Hülya Asan Anıl Özyurt , Hakan Allı Ahmet Asan Hakan Nazlı Önder Sarp

Öz

Background and Aims: Fomes fomentarius (L.) Fr. (jar fungus) has been used extensively in the past by surgeons, barbers, dentists, and is therefore called surgeon's fungus. The hemostatic properties of F. fomentarius extracts are important in medicine. The aim of this study was to investigate the effect of the gel formulation obtained from Fomes Fomentarius on bleeding and clotting time in rat models. Methods: The gel was made for this purpose and a cross-linked polymer of acrylic acid was used as a gelling agent. Results: The outcomes of the characterization analysis indicate the development of a successful gel formulation with optimum characteristics. Data from the antimicrobial study showed a good inhibition zone, with the strongest values against Klebsiella pneumoniae, Candida krusei, C. tropicalis, C. guilliermondii. The gel administration shortened the hemostasis time after bleeding from skin incisions by 63.9% and tail incisions by 55.63% from the original time. For coagulation time, these results are determined as 71.56% from the original time. The gel administration shortened the coagulation time after bleeding from the tail incisions by 69.9 % from the original time. Conclusion: Our preliminary study showed that the gel formulation obtained from F. fomentarius is a potent hemostatic agent in a rat-bleeding model.

Anahtar Kelimeler

Fomes fomentarius, bleeding time, coagulation time

Kaynakça

• • Akramiene, D., Kondrotas, A., Didziapetriene, J. & Kevelaitis, E. (2007). Effects of beta-glucans on the immune system. Medicina, 43(8), 597–606. https://doi.org/10.3390/medicina43080076
• • Allı, H., Işıloğlu, M. & Solak, M. H. (2007). Macrofungi of Aydın Province. Mycotaxon, 99(1), 163–165. Retrieved from https://www.researchgate. net/publication/282726082_Macrofungi_of_Aydin_ Province_Turkey_65/link/5c8966b5a6fdcc3817526251.
• • Carr, J. A., Silverman, N. (1999). The heparin-protamine interaction: A review. The Journal of Cardiovascular Surgery, 40(5), 659–666. Retrieved from https://europepmc.org/article/med/10596998.
• • Carvalho, F. C., Barb, M. S., Sarmento, V. H. V., Chiavacci, L. A., Netto, F. M. & Gremiao, M. P. D. (2010). Surfactant systems for nasal zidovudine delivery structural, rheological and mucoadhesive properties. Journal of Pharmacy Pharmacology, 62(4), 430–439. https:// doi.org/10.1211/jpp.62.04.0004.
• • Chen, W, Zhao Z, Li Y. (2011). Simultaneous increase of mycelial biomass and intracellular polysaccharide from Fomes fomentarius and its biological function of gastric cancer intervention. Carbohydrate Polymers, 85(2), 369–375. https://doi.org/10.1016/j.carbpol. 2011.02.035.
• • Cömert, M., Karakaya, K, Barut, F., Çakmak, K. G., Uçan, H. B., Gültekin, F. A., Emre, A. U., Taşçılar, Ö., Irkörücü, O., Ankaralı, H. (2010). Does intraabdominal use of Ankaferd Blood Stopper cause increased intraperitoneal adhesions. National Trauma and Emergency of Surgery Journal, 16(5), 383–389. Retrieved from https:// pubmed.ncbi.nlm.nih.gov/21038113.
• • Dresch, P., D´Aguanno, M. N., Rosam, K., Grienke, U., Rollinger, J. M., Ursula Peintner, U. (2015). Fungal strain matters: colony growth and bioactivity of the European medicinal polypores Fomes fomentarius, Fomitopsis pinicola and Piptoporus betulinus. AMB Express, 5(4). https://doi.org/10.1186/s13568-014-0093-0.
• • Ellis, M. B. & Ellis, J. P. (1990). Fungi without gills (Hymenomycetes and Gasteromycetes). An Identification Handbook. XI, 329 S., 543 Abb. Chapman and Hall, London, New York, Tokyo, Melbourne, Madras, 1990. ISBN 0‐412‐36970‐2
• • Ereth-Mark, H., Schaft, M., Ericson, E., Wetgen, N., Nuttal, G., Oliver, W. C. (2008). Comparative safety and efficacy of topical hemostatic agents in a rat neurosurgical model. Neurosurgery, 63, 369–372. https://doi.org/10.1227/01.neu.0000327031.98098.dd.
• • Gedik, G., Dülger, G., Asan, H., Özyurt, A., Allı, H. & Asan A. (2019). The antimicrobial effect of various formulations obtained from Fomes fomentarius against hospital isolates. Mantar Dergisi- The Journal of Fungus, 10(2), 103–109. https://doi.org/ 10.30708/mantar. 535994.
• • Grienke, U., Zöll, M., Peintner, U. & Rollinger, J. M. (2014). European medicinal polypores–A modern view on traditional uses. Journal of Ethnopharmacology, 154(3), 564–583. https://doi.org/10.1016/j. jep.2014.04.030.
• • Güven, R. (2019). An experimental study investigating the effect of local hemostatic agents on hemostatic, histopathological, antimullerian hormone and postoperative intraabdominal adhesion in a laceration model of complicated ovarian hyperstimulation (Expertise thesis in medicine). Available from tez.yok.gov.tr. (No. 535721).
• • Kaur, L. P., Guleri, T. K. (2013). Topical Gel: A Recent Approach for Novel Drug delivery. Asian Journal of Biomedical and Pharmaceutical Sciences, 3(17), 1–5. https://doi.org/10.15272/AJBPS.V3I17.183.
• • Kheirabadi, B. S. (2011). Evaluation of topical hemostatic agents for combat wound treatment. The United States Army Medical Department Journal, 2, 25–38. Retrieved from https://pubmed.ncbi. nlm.nih.gov/21607904.
• • Kolundžić, M., Grozdanić, N. D., Dodevska, M., Milenković, M., Sisto, F., Miani, A. & Kundaković, T. (2016). Antibacterial and cytotoxic activities of wild mushroom Fomes fomentarius (L.) Fr. Polyporaceae. Industrial Crops and Products, 79, 110–115. https://doi. org/10.1016/j.indcrop.2015.10.030.
• • Lindstrom, P. A. (1956). Complications from the use of absorbable hemostatic sponges. American Medical Association Archive of Surgery, 73(1), 133–141. https://doi.org/10.1001/archsurg. 1956.01280010135018.
• • Lu, J., Meng, H., Meng, Z., Sun, Y., Pribis, J. P., Zhu, C., Li, Q. (2015). Epsilon aminocaproic acid reduces blood transfusion and improves the coagulation test after pediatric open-heart surgery: A metaanalysis of 5 clinical trials. International Journal of Clinical and Experimental Pathology, 8(7), 7978–7987. Retrieved from https:// pubmed.ncbi.nlm.nih.gov/26339364.
• • Napolitano, L. M., Cohen, M. J., Cotton, B. A., Schreiber, M. A., Moore, E. E. (2013). Tranexamic acid in trauma: how should we use it ? Journal of Trauma and Acute Care Surgery, 74(6), 1575–86. https://doi.org/10.1097/ta.0b013e318292cc54.
• • Nilsson, I. M., Magnusson, S., Borchgrevink, C. (1963).The Duke and Ivy methods for determination of the bleeding time. Thrombosis et Diathesis Haemorrhagica 10.02: 223–234. Retrieved from https://europepmc.org/article/med/14081283
• • Okur, N. Ü., Çağlar, E. Ş., Arpa, M. D. & Karasulu, H. Y. (2017). Preparation and evaluation of novel microemulsion-based hydrogels for dermal delivery of benzocaine. Pharmaceutical Development and Technology, 22(4), 500–510. https://doi.org/10.3109/10837450.2015.1131716.
• • Okur, N. Ü., Yoltaş, A. & Yozgatli, V. (2016). Development and characterization of voriconazole loaded in situ gel formulations for ophthalmic application. Turkish Journal of Pharmaceutical Sciences, 13(3): 311–317. https://doi.org/10.4274/tjps.2016.05
• • Pacioni, G. (1985). Mac Ency Mushrooms Toadstools (Macdonald encyclopedias). London.
• • Park, Y. M., Kim, I. T., Park, H. J., Choi, J. W., Park, K. Y., Lee, J. D., Nam, B. H., Kim, D. G., Lee, J. Y. & Lee, K. T. (2004). Anti-inflammatory and anti-nociceptive effects of the methanol extract of Fomes fomentarius. Biological & Pharmaceutical Bulletin, 27(10), 1588–1593. https://doi.org/10.1248/bpb.27.1588.
• • Pathak, A., Hamm, C. R., Eyal, F. G., Walter, K., Rijhsinghani, A., Bohlman, M. (1990). Maternal Vitamin K administration for prevention of intraventricular hemorrhage in preterm infants. Pediatric Research, 27, 219. Retrieved from https://www.ncbi.nlm.nih.gov/ pmc/articles/PMC7043360.
• • Pusateri, A. E., McCarthy, S. J., Gregory, K. W., Harris, R. A., Cardenas, L., McManus, A. T., & Goodwin Jr, C. W. (2003). Effect of a chitosanbased hemostatic dressing on blood loss and survival in a model of severe venous hemorrhage and hepatic injury in swine. Journal of Trauma and Acute Care Surgery, 54(1), 177–182. https://doi. org/10.1097/00005373-200301000-00023.