Background and Aims: An HPLC method with photodiode array detector on a chiral column was proposed for enantioselective determination of chlorpheniramine (CLP) enantiomers in dosage forms. Methods: The enantioselective determination was achieved on amylose tris(3,5-dimethylphenylcarbamate) column, using n-hexane-(propan-2-ol)-diethylamine (97.5:2.5:0.025, v/v/v) mobile phase. The peaks were detected at 258 nm. Diphenhydramine was used as an internal standard (IS). A new sample preparation procedure was developed to avoid the interference of the other ingredients present in the formulations. Results: Limit of quantification of the proposed method was 0.88 and 1.31 μg/mL for S-(+)-CLP and R-(-)-CLP, respectively. Conclusion: The method is linear, sensitive, specific and can be used for the enantioselective assay of CLP enantiomers in pharmaceutical formulations.
Chlorpheniramine amylose tris(3,5-dimethyl phenylcarbamate) enantioselective determination HPLC-PDA chirality
Birincil Dil | İngilizce |
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Konular | Eczacılık ve İlaç Bilimleri, Sağlık Kurumları Yönetimi |
Bölüm | Short Paper |
Yazarlar | |
Yayımlanma Tarihi | 27 Ağustos 2020 |
Gönderilme Tarihi | 4 Aralık 2019 |
Yayımlandığı Sayı | Yıl 2020 Cilt: 50 Sayı: 2 |