Effect of Sildenafil Citrate on MDA, GSH, Retinol, Vitamin D3 and α-Tocoferole Levels in Wound Healing: Diabetic Rat Model

Abstract

In this study, the effect of sildenafil citrate (SC) on Malondialdehyde (MDA), Glutathione (GSH), retinol, vitamin D3 and α-tocoferole levels were researched in the rats with diabetes and wound formation. 3 groups were formed in the study and 10 male Swiss albino rats with the weight of 250-300 g were used in each group. Wound implementation was conducted on the rats of the 1st group, diabetes and wound implementations on the rats of the 2nd group and diabetes, wound and SC implementations on the rats of the 3rd group. Normal wound care was applied to the first two groups; besides the normal wound care, SC was also applied to the third group intraperitoneally (i.p.) at 0.7 mg/kg once a day and during 3 days. Blood samples were taken before (day 0) and after (9th day) the applications. MDA and GSH were checked in the whole blood, and retinol, vitamin D3 and α-tocoferole levels were examined in the plasma. At the end of the study, MDA values of the third group were found lower (P< 0.05), GSH plasma retinol and α-tocoferole levels higher (P< 0.05) than the other groups. Consequently, it was determined that application of SC in appropriate doses and time might have a positive effect on enabling the oxidant and antioxidant balance during the wound healing in diabetes patients

Keywords

• Diabetes, Wound, Sildenafil Citrate, MDA, GSH, retinol, α-tocoferole