EN
Screening the Tomato (Lycopersicum esculentum Mill.) Lines for the Resistance to Root-knot Nematodes and Verticillium Wilt by DNA Markers
Abstract
In this study, it was aimed to determine the resistance to root-knot nematodes and Verticillium wilt of 288 tomato (Lycopersicum esculentum Mill.) samples by DNA markers. First, it was established that whether the tomato samples have primer binding site or not by the CAPS primers which are specifically designed for both of the diseases. In the analysis of primer binding site to root-knot nematodes and Verticillium wilt diseases, it is found that in the 288 tomato samples, 247 and 235 tomato samples have specific primer binding site respectively. Then, restriction enzymes (TaqI and HincII) were used to understand whether the individuals were homozygote or heterozygote resistant, or susceptible to these diseases for determination of resistance to root-knot nematodes and Verticillium wilt. It is found that 200 samples were susceptible, 45 samples were heterozygote resistant and 2 samples were homozygote resistant to root-knot nematodes, and 125 samples were susceptible, 110 samples were heterozygote to Verticillium wilt
Keywords
Details
Primary Language
English
Subjects
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Journal Section
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Publication Date
July 1, 2010
Submission Date
July 1, 2010
Acceptance Date
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Published in Issue
Year 1970 Volume: 4 Number: 2
APA
Soya, S., & Tanyolac, B. (2010). Screening the Tomato (Lycopersicum esculentum Mill.) Lines for the Resistance to Root-knot Nematodes and Verticillium Wilt by DNA Markers. Journal of Applied Biological Sciences, 4(2), 39-44. https://izlik.org/JA43WR25JL
AMA
1.Soya S, Tanyolac B. Screening the Tomato (Lycopersicum esculentum Mill.) Lines for the Resistance to Root-knot Nematodes and Verticillium Wilt by DNA Markers. J.appl.biol.sci. 2010;4(2):39-44. https://izlik.org/JA43WR25JL
Chicago
Soya, Seçkin, and Bahattin Tanyolac. 2010. “Screening the Tomato (Lycopersicum Esculentum Mill.) Lines for the Resistance to Root-Knot Nematodes and Verticillium Wilt by DNA Markers”. Journal of Applied Biological Sciences 4 (2): 39-44. https://izlik.org/JA43WR25JL.
EndNote
Soya S, Tanyolac B (July 1, 2010) Screening the Tomato (Lycopersicum esculentum Mill.) Lines for the Resistance to Root-knot Nematodes and Verticillium Wilt by DNA Markers. Journal of Applied Biological Sciences 4 2 39–44.
IEEE
[1]S. Soya and B. Tanyolac, “Screening the Tomato (Lycopersicum esculentum Mill.) Lines for the Resistance to Root-knot Nematodes and Verticillium Wilt by DNA Markers”, J.appl.biol.sci., vol. 4, no. 2, pp. 39–44, July 2010, [Online]. Available: https://izlik.org/JA43WR25JL
ISNAD
Soya, Seçkin - Tanyolac, Bahattin. “Screening the Tomato (Lycopersicum Esculentum Mill.) Lines for the Resistance to Root-Knot Nematodes and Verticillium Wilt by DNA Markers”. Journal of Applied Biological Sciences 4/2 (July 1, 2010): 39-44. https://izlik.org/JA43WR25JL.
JAMA
1.Soya S, Tanyolac B. Screening the Tomato (Lycopersicum esculentum Mill.) Lines for the Resistance to Root-knot Nematodes and Verticillium Wilt by DNA Markers. J.appl.biol.sci. 2010;4:39–44.
MLA
Soya, Seçkin, and Bahattin Tanyolac. “Screening the Tomato (Lycopersicum Esculentum Mill.) Lines for the Resistance to Root-Knot Nematodes and Verticillium Wilt by DNA Markers”. Journal of Applied Biological Sciences, vol. 4, no. 2, July 2010, pp. 39-44, https://izlik.org/JA43WR25JL.
Vancouver
1.Seçkin Soya, Bahattin Tanyolac. Screening the Tomato (Lycopersicum esculentum Mill.) Lines for the Resistance to Root-knot Nematodes and Verticillium Wilt by DNA Markers. J.appl.biol.sci. [Internet]. 2010 Jul. 1;4(2):39-44. Available from: https://izlik.org/JA43WR25JL