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Genetic Polymorphism of Whey Protein Genes β-LG and α-LA in Three Egyptian Sheep Breeds

Yıl 2012, Cilt: 6 Sayı: 3, 25 - 30, 01.11.2012

Öz

The major whey proteins, β-lactoglobulin (β-LG) and α-lactalbumin (α-LA), are strongly correlated with the nutritional value and the functional properties of milk whey. Ovine whey has a unique protein composition compared to that of bovine whey and it is especially rich in proteins. Genetic polymorphism of β-LG using PCR-RFLP and α-LA using PCR-SSCP was analyzed in three native Egyptian sheep breeds named Barki, Rahmani and Ossimi. Digestion of resulting amplified fragments of β-LG (452-bp) with RsaI endonuclease differentiated between three different genotypes AA (69.77%), AB (26.74%) and BB (3.49%). The results showed that A allele was present in the Egyptian sheep breeds with a frequency (83.14%) higher than B allele (16.86%). A & B alleles appeared at frequencies 70.4 % & 29.6%, 95.0% & 5.0% and 82.8% & 17.2% in Barki, Rahmani and Ossimi, respectivelySSCP results of the α-LA amplified fragments (166-bp) showed that the three native Egyptian sheep breeds possess two different patterns related to the difference in nucleotide sequences with frequencies 88.1% and 11.9%. The frequencies of pattern 1 ranged from 81.48% in Barki to 89.66% in Ossimi and 92.86% in Rahmani breeds whereas the pattern 2 was present at frequencies 18.52%, 7.14% and 10.34% in Barki, Rahmani and Ossimi breeds, respectively

Yıl 2012, Cilt: 6 Sayı: 3, 25 - 30, 01.11.2012

Öz

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Ayrıntılar

Diğer ID JA86AU77MN
Bölüm Araştırma Makalesi
Yazarlar

Othman E. Othman Bu kişi benim

Samia A. El-fıky Bu kişi benim

Nagwa A. Hassan Bu kişi benim

Eman R. Mahfouz Bu kişi benim

Esraa A. Balabel Bu kişi benim

Yayımlanma Tarihi 1 Kasım 2012
Yayımlandığı Sayı Yıl 2012 Cilt: 6 Sayı: 3

Kaynak Göster

APA Othman, O. E., El-fıky, S. A., Hassan, N. A., Mahfouz, E. R., vd. (2012). Genetic Polymorphism of Whey Protein Genes β-LG and α-LA in Three Egyptian Sheep Breeds. Journal of Applied Biological Sciences, 6(3), 25-30.
AMA Othman OE, El-fıky SA, Hassan NA, Mahfouz ER, Balabel EA. Genetic Polymorphism of Whey Protein Genes β-LG and α-LA in Three Egyptian Sheep Breeds. J.appl.biol.sci. Kasım 2012;6(3):25-30.
Chicago Othman, Othman E., Samia A. El-fıky, Nagwa A. Hassan, Eman R. Mahfouz, ve Esraa A. Balabel. “Genetic Polymorphism of Whey Protein Genes β-LG and α-LA in Three Egyptian Sheep Breeds”. Journal of Applied Biological Sciences 6, sy. 3 (Kasım 2012): 25-30.
EndNote Othman OE, El-fıky SA, Hassan NA, Mahfouz ER, Balabel EA (01 Kasım 2012) Genetic Polymorphism of Whey Protein Genes β-LG and α-LA in Three Egyptian Sheep Breeds. Journal of Applied Biological Sciences 6 3 25–30.
IEEE O. E. Othman, S. A. El-fıky, N. A. Hassan, E. R. Mahfouz, ve E. A. Balabel, “Genetic Polymorphism of Whey Protein Genes β-LG and α-LA in Three Egyptian Sheep Breeds”, J.appl.biol.sci., c. 6, sy. 3, ss. 25–30, 2012.
ISNAD Othman, Othman E. vd. “Genetic Polymorphism of Whey Protein Genes β-LG and α-LA in Three Egyptian Sheep Breeds”. Journal of Applied Biological Sciences 6/3 (Kasım 2012), 25-30.
JAMA Othman OE, El-fıky SA, Hassan NA, Mahfouz ER, Balabel EA. Genetic Polymorphism of Whey Protein Genes β-LG and α-LA in Three Egyptian Sheep Breeds. J.appl.biol.sci. 2012;6:25–30.
MLA Othman, Othman E. vd. “Genetic Polymorphism of Whey Protein Genes β-LG and α-LA in Three Egyptian Sheep Breeds”. Journal of Applied Biological Sciences, c. 6, sy. 3, 2012, ss. 25-30.
Vancouver Othman OE, El-fıky SA, Hassan NA, Mahfouz ER, Balabel EA. Genetic Polymorphism of Whey Protein Genes β-LG and α-LA in Three Egyptian Sheep Breeds. J.appl.biol.sci. 2012;6(3):25-30.