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Phytase Characterization and Production from Lactobacillus plantarum Strain on Corn Steep Liquor

Year 2016, Volume: 10 Issue: 2, 64 - 66, 01.07.2016

Fatima Masume Uslu Ecem Gizem Kızılkaya Esra Sunduz Yiğittekin Mustafa Gençoğlu Sevil Toroğlu Sadik Dinçer

Abstract

In this study, effect of corn steep liquor on phytase enzyme production from Lactobacillus plantarum strain isolated from fermented food products was investigated and produced phytase was characterized. The enzyme produced in MRS broth containing %30 corn steep liquor and %0,2 glucose for 24 hours at 30°C.The intracellular enzyme isolated from Lactobacillus plantarum strain used in this study. The optimum activity of enzyme was emerged at 40°C and pH 5. This enzyme was 92% stable for 30 minutes at 100°C. Enzyme activity decreased with 1mM concetration of MgCI2 (3.6%), CoCI2 (13.5%) HgCl2 (14%), NiCI2 (20%), MnCl2 (15%), CaCI2 (15%), ZnCl2 (9%), CuCl2 (24%), FeCl2 (15%) and EDTA (13%) but activity increased with 5mM concentration of MgCI2 (5%), CoCI2 (13.5%) HgCl2 (22%), NiCI2 (16%), MnCl2 (18%), CaCI2 (20%), ZnCl2 (39%), CuCl2 (10%), FeCl2 (10%) and EDTA (12%). As a result, it is seen that due to the characteristic features of the produced pytase enzyme can be proposed that it is convenient to be used in Industrial area

Keywords

Lactobacillus plantarum Phytase Corn steep liquor Phytase characterization

Year 2016, Volume: 10 Issue: 2, 64 - 66, 01.07.2016

Fatima Masume Uslu Ecem Gizem Kızılkaya Esra Sunduz Yiğittekin Mustafa Gençoğlu Sevil Toroğlu Sadik Dinçer

Abstract

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Details

Other ID JA74GS93CE
Journal Section Research Article
Authors

Fatima Masume Uslu This is me

Ecem Gizem Kızılkaya This is me

Esra Sunduz Yiğittekin This is me

Mustafa Gençoğlu This is me

Sevil Toroğlu This is me

Sadik Dinçer This is me

Publication Date July 1, 2016
Published in Issue Year 2016 Volume: 10 Issue: 2

Cite

APA Uslu, F. M., Kızılkaya, E. G., Yiğittekin, E. S., Gençoğlu, M., et al. (2016). Phytase Characterization and Production from Lactobacillus plantarum Strain on Corn Steep Liquor. Journal of Applied Biological Sciences, 10(2), 64-66.
AMA Uslu FM, Kızılkaya EG, Yiğittekin ES, Gençoğlu M, Toroğlu S, Dinçer S. Phytase Characterization and Production from Lactobacillus plantarum Strain on Corn Steep Liquor. J.appl.biol.sci. July 2016;10(2):64-66.
Chicago Uslu, Fatima Masume, Ecem Gizem Kızılkaya, Esra Sunduz Yiğittekin, Mustafa Gençoğlu, Sevil Toroğlu, and Sadik Dinçer. “Phytase Characterization and Production from Lactobacillus Plantarum Strain on Corn Steep Liquor”. Journal of Applied Biological Sciences 10, no. 2 (July 2016): 64-66.
EndNote Uslu FM, Kızılkaya EG, Yiğittekin ES, Gençoğlu M, Toroğlu S, Dinçer S (July 1, 2016) Phytase Characterization and Production from Lactobacillus plantarum Strain on Corn Steep Liquor. Journal of Applied Biological Sciences 10 2 64–66.
IEEE F. M. Uslu, E. G. Kızılkaya, E. S. Yiğittekin, M. Gençoğlu, S. Toroğlu, and S. Dinçer, “Phytase Characterization and Production from Lactobacillus plantarum Strain on Corn Steep Liquor”, J.appl.biol.sci., vol. 10, no. 2, pp. 64–66, 2016.
ISNAD Uslu, Fatima Masume et al. “Phytase Characterization and Production from Lactobacillus Plantarum Strain on Corn Steep Liquor”. Journal of Applied Biological Sciences 10/2 (July 2016), 64-66.
JAMA Uslu FM, Kızılkaya EG, Yiğittekin ES, Gençoğlu M, Toroğlu S, Dinçer S. Phytase Characterization and Production from Lactobacillus plantarum Strain on Corn Steep Liquor. J.appl.biol.sci. 2016;10:64–66.
MLA Uslu, Fatima Masume et al. “Phytase Characterization and Production from Lactobacillus Plantarum Strain on Corn Steep Liquor”. Journal of Applied Biological Sciences, vol. 10, no. 2, 2016, pp. 64-66.
Vancouver Uslu FM, Kızılkaya EG, Yiğittekin ES, Gençoğlu M, Toroğlu S, Dinçer S. Phytase Characterization and Production from Lactobacillus plantarum Strain on Corn Steep Liquor. J.appl.biol.sci. 2016;10(2):64-6.