Transcriptomic Analysis of Yarrowia lipolytica YlNTH1 and YlTPS1 Genes Under Different Carbon Sources

Year 2018, Volume: 12 Issue: 3, 1 - 3, 26.12.2018

Tulay Genc , Burak Servılı

Abstract

The biosynthesis of trehalose is catalyzed by synthase enzyme complex
and degraded by neutral trehalase enzyme. Yarrowia lipolytica is a dimorphic yeast and used as a model
organism for understanding the lipid metabolism of higher eukaryotes. YlTPS1 and YlNTH1 genes are responsible
from the recycling of trehalose in Y. lipolytica. The aim of this work is to determine the
expression patterns of YlTPS1 and YlNTH1 genes
under different carbon sources by using RNAseq study. The
files used in this analysis were obtained from the EMBL-EBI data bank and Study ID is “PRJEB2863”. The
substrates used in the work are Alkane, Glucose, Glycerol, Oleic Acid,
Tributyrin and Triolein. The run accession numbers of substrates are ERR073010,
ERR073011, ERR073009, ERR073008, ERR073012 and ERR073007, respectively.
"Trimmomatic" tool was used for croping the readings in the next
generation sequencing files. Reference genome of Y. lipolytica were obtained from Ensembl genome data base. The
files were converted to “ERR0730xx_tophat2.bam” files and used in the
"FeatureCounts" tool. We found that the transcript level of YlTPS1 gene was 2 to 4 times higher than
YlNTH1 gene. The rate of trehalose synthesis
is greater than the rate of breakdown in Y.
lipolytica yeast cells.

Keywords

YlTPS1, YlNTH1, RNAseq, Yarrowia lipolytica

References

• Reference 1 Prof.Dr. Sezai Türkel Uludağ Üniversitesi, Fen Edebiyat Fakültesi, Moeküler Biyoloji ve Genetik Bölümü, Görükle/BURSAe-mail:[e-mail]: 02242941804cep tel:[phone]
• Reference 2Prof.Dr. Serap MUTUN, Abant İzzet Baysal Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Gölköy/BOLU e-mail:[e-mail]: 0374 2541000 ext.1355cep tel:[phone]