Phytochemical Characterization of Dittrichia viscosa Olive Oil Macerate and Investigation of in vitro Anticancer Activity

Buse Aktaş; Erdal Eroğlu; İlker Polatoglu

doi:10.28979/jarnas.1916612

Phytochemical Characterization of Dittrichia viscosa Olive Oil Macerate and Investigation of in vitro Anticancer Activity

Abstract

Dittrichia viscosa, known as false yellowhead, is one of the natural medicinal plants displaying anti-inflammatory, antiproliferative, and anticancer effects. One of the traditional methods, used since ancient times, is the oily maceration of herbs. In this study, for the first time, the content analysis and anticancer activities of extracts obtained from olive oil macerates of D. viscosa leaves and flowers were investigated in vitro. Using DMSO, extracts from i) olive oil as control (Doo) or macerates of ii) flowers of D. viscosa (D-Dv-F), iii) leaves of D. viscosa (D-Dv-L) were obtained. Phytochemicals, total phenolic contents, antioxidant capacities, and flavonoid contents of the extracts were determined using the LC-MS/MS analysis, Folin-Ciocalteu method, CUPRAC assay, and aluminum chloride calorimetric method, respectively. The in vitro anticancer activities of DMSO extracts were also determined using an MTT cell proliferation assay against human bladder cancer cells (T-24) at concentrations of 0.05%-1% (v/v). According to the MTT cell proliferation assay, D-Dv-L, with a higher phenolic content and antioxidant activity than D-Dv-F and D-oo, exhibited in vitro anticancer activity. The obtained findings are promising for testing the anticancer activity of D-Dv-L in vivo.

Keywords

Supporting Institution

Manisa Celal Bayar University

Project Number

This work is not a part of any project.

Ethical Statement

No approval from the Board of Ethics is required.

Thanks

We thank BIOSENS ID A.Ş. and Cemal İZCİ for their financial support. The authors would also like to thank Assoc. Prof. Dr. Dilek OSKAY at Manisa Celal Bayar University, Department of Biology, for the identification of plant material.

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Details

Primary Language

English

Subjects

Bioprocess

Journal Section

Research Article

Authors

Buse Aktaş
0009-0009-0423-4086
Türkiye

Erdal Eroğlu ^*
0000-0001-7281-3906
Türkiye

İlker Polatoglu
0000-0002-6099-062X
Türkiye

Publication Date

June 30, 2026

Submission Date

March 26, 2026

Acceptance Date

April 17, 2026

Published in Issue

Year 2026 Volume: 12 Number: 2

DOI

https://doi.org/10.28979/jarnas.1916612

IZ

https://izlik.org/JA97BS36FW

Cite

RIS / Bibtex

APA

Aktaş, B., Eroğlu, E., & Polatoglu, İ. (2026). Phytochemical Characterization of Dittrichia viscosa Olive Oil Macerate and Investigation of in vitro Anticancer Activity. Journal of Advanced Research in Natural and Applied Sciences, 12(2), 133-145. https://doi.org/10.28979/jarnas.1916612

AMA

1.Aktaş B, Eroğlu E, Polatoglu İ. Phytochemical Characterization of Dittrichia viscosa Olive Oil Macerate and Investigation of in vitro Anticancer Activity. JARNAS. 2026;12(2):133-145. doi:10.28979/jarnas.1916612

Chicago

Aktaş, Buse, Erdal Eroğlu, and İlker Polatoglu. 2026. “Phytochemical Characterization of Dittrichia Viscosa Olive Oil Macerate and Investigation of in Vitro Anticancer Activity”. Journal of Advanced Research in Natural and Applied Sciences 12 (2): 133-45. https://doi.org/10.28979/jarnas.1916612.

EndNote

Aktaş B, Eroğlu E, Polatoglu İ (June 1, 2026) Phytochemical Characterization of Dittrichia viscosa Olive Oil Macerate and Investigation of in vitro Anticancer Activity. Journal of Advanced Research in Natural and Applied Sciences 12 2 133–145.

IEEE

[1]B. Aktaş, E. Eroğlu, and İ. Polatoglu, “Phytochemical Characterization of Dittrichia viscosa Olive Oil Macerate and Investigation of in vitro Anticancer Activity”, JARNAS, vol. 12, no. 2, pp. 133–145, June 2026, doi: 10.28979/jarnas.1916612.

ISNAD

Aktaş, Buse - Eroğlu, Erdal - Polatoglu, İlker. “Phytochemical Characterization of Dittrichia Viscosa Olive Oil Macerate and Investigation of in Vitro Anticancer Activity”. Journal of Advanced Research in Natural and Applied Sciences 12/2 (June 1, 2026): 133-145. https://doi.org/10.28979/jarnas.1916612.

JAMA

1.Aktaş B, Eroğlu E, Polatoglu İ. Phytochemical Characterization of Dittrichia viscosa Olive Oil Macerate and Investigation of in vitro Anticancer Activity. JARNAS. 2026;12:133–145.

MLA

Aktaş, Buse, et al. “Phytochemical Characterization of Dittrichia Viscosa Olive Oil Macerate and Investigation of in Vitro Anticancer Activity”. Journal of Advanced Research in Natural and Applied Sciences, vol. 12, no. 2, June 2026, pp. 133-45, doi:10.28979/jarnas.1916612.

Vancouver

1.Buse Aktaş, Erdal Eroğlu, İlker Polatoglu. Phytochemical Characterization of Dittrichia viscosa Olive Oil Macerate and Investigation of in vitro Anticancer Activity. JARNAS. 2026 Jun. 1;12(2):133-45. doi:10.28979/jarnas.1916612

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