Yıl 2020, Cilt 4 , Sayı 3, Sayfalar 230 - 236 2020-09-01

Assessment of Rac1 and β-PAK Expressions in a Mouse Model for Contrast-Induced Nephropathy

Yasar Aysun MANİSALİGİL [1] , Serap CİLAKER MİCİLİ [2] , Servet KİZİLDAG [3] , Aslı CELİK [4] , Husnu Alper BAGRİYANİK [5] , Mukaddes GUMUSTEKİN [6]


Purpose: Radiocontrast-induced nephropathy is an important clinical problem in high-risk patients. The mechanism of cytotoxic effect of radiocontrast media on kidneys is still not fully clarified. Rac1/β-PAK pathway has been shown to play a role in other nephropathies such as diabetic nephropathy. In this study, it was aimed to determine whether Rac1/β-PAK signalling pathway has any role in the development of contrast-induced nephropathy CIN . Methods: Adult male Balb/C mice were used. A single dose of iohexol was given intraperitoneally. Thirty-two mice were divided into 5 groups including control Group1, n=4 , pretreatment Group 2, n=7 , low dose 2 g iodine/kg iohexol Group 3, n=7 , medium dose 2.5 g iodine/kg iohexol Group 4, n=7 and high dose 3 g iodine/kg iohexol Group 5, n=7 . The animals were sacrificed 24 hours after iohexol administration. Nephropathy were evaluated by histological and biochemical analysis methods. Expressions of Rac1 and β-PAK were evaluated by immunohistochemical method. Apoptosis was assessed by TUNEL method. Results: It was found that severity of nephropathy, apoptosis and β-PAK expressions significantly increased as iohexol dosage increased. Although Rac1 expression was higher in the iohexol group in comparison to the control and pretreatment groups, its increase did not show a dose-dependent manner. There was no significant difference among the groups in terms of serum creatinine. Serum cystatin C were increased in all groups compared to the control group, but significant increase was observed only in the pretreatment group. Conclusions: Our findings suggest that the Rac1/β-PAK signal transduction pathway may have a role in the contrast-induced nephropathy model.
apoptosis, contrast-induced nephropathy, iohexol, mouse, Rac1
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Birincil Dil en
Bölüm Research Article
Yazarlar

Yazar: Yasar Aysun MANİSALİGİL
Kurum: Dokuz Eylul University, Vocational School of Health Services, Medical Imaging Techniques Program, Izmir, Turkey

Yazar: Serap CİLAKER MİCİLİ
Kurum: Dokuz Eylul University, Institute of Health Sciences, Department of Histology and Embrylogy, Izmir, Turkey

Yazar: Servet KİZİLDAG
Kurum: Dokuz Eylul University,Vocational School of Health Services, Medical Laboratory Techniques Program, Izmir, Turkey

Yazar: Aslı CELİK
Kurum: Dokuz Eylul University, Faculty of Medicine, Department of Laboratory Animal Science,, Izmir, Turkey

Yazar: Husnu Alper BAGRİYANİK
Kurum: Izmir International Biomedicine and Genome Intitute, (iBG-izmir), Izmir, Turkey

Yazar: Mukaddes GUMUSTEKİN
Kurum: Dokuz Eylul University, Faculty of Medicine, Department of Pharmacology, Izmir, Turkey

Tarihler

Başvuru Tarihi : 25 Haziran 2021
Kabul Tarihi : 25 Haziran 2021
Yayımlanma Tarihi : 1 Eylül 2020

Bibtex @ { jbachs844388, journal = {Journal of Basic and Clinical Health Sciences}, issn = {}, eissn = {2564-7288}, address = {Dokuz Eylül Üniversitesi Sağlık Bilimleri Enstitüsü İnciraltı İzmir 35340}, publisher = {Dokuz Eylül Üniversitesi}, year = {2020}, volume = {4}, pages = {230 - 236}, doi = {10.30621/jbachs.2020.1057}, title = {Assessment of Rac1 and β-PAK Expressions in a Mouse Model for Contrast-Induced Nephropathy}, key = {cite}, author = {Manisaligil, Yasar Aysun and Cilaker Micili, Serap and Kizildag, Servet and Celik, Aslı and Bagriyanik, Husnu Alper and Gumustekin, Mukaddes} }
APA Manisaligil, Y , Cilaker Micili, S , Kizildag, S , Celik, A , Bagriyanik, H , Gumustekin, M . (2020). Assessment of Rac1 and β-PAK Expressions in a Mouse Model for Contrast-Induced Nephropathy . Journal of Basic and Clinical Health Sciences , 4 (3) , 230-236 . DOI: 10.30621/jbachs.2020.1057
MLA Manisaligil, Y , Cilaker Micili, S , Kizildag, S , Celik, A , Bagriyanik, H , Gumustekin, M . "Assessment of Rac1 and β-PAK Expressions in a Mouse Model for Contrast-Induced Nephropathy" . Journal of Basic and Clinical Health Sciences 4 (2020 ): 230-236 <https://dergipark.org.tr/tr/pub/jbachs/issue/58450/844388>
Chicago Manisaligil, Y , Cilaker Micili, S , Kizildag, S , Celik, A , Bagriyanik, H , Gumustekin, M . "Assessment of Rac1 and β-PAK Expressions in a Mouse Model for Contrast-Induced Nephropathy". Journal of Basic and Clinical Health Sciences 4 (2020 ): 230-236
RIS TY - JOUR T1 - Assessment of Rac1 and β-PAK Expressions in a Mouse Model for Contrast-Induced Nephropathy AU - Yasar Aysun Manisaligil , Serap Cilaker Micili , Servet Kizildag , Aslı Celik , Husnu Alper Bagriyanik , Mukaddes Gumustekin Y1 - 2020 PY - 2020 N1 - doi: 10.30621/jbachs.2020.1057 DO - 10.30621/jbachs.2020.1057 T2 - Journal of Basic and Clinical Health Sciences JF - Journal JO - JOR SP - 230 EP - 236 VL - 4 IS - 3 SN - -2564-7288 M3 - doi: 10.30621/jbachs.2020.1057 UR - https://doi.org/10.30621/jbachs.2020.1057 Y2 - 2021 ER -
EndNote %0 Journal of Basic and Clinical Health Sciences Assessment of Rac1 and β-PAK Expressions in a Mouse Model for Contrast-Induced Nephropathy %A Yasar Aysun Manisaligil , Serap Cilaker Micili , Servet Kizildag , Aslı Celik , Husnu Alper Bagriyanik , Mukaddes Gumustekin %T Assessment of Rac1 and β-PAK Expressions in a Mouse Model for Contrast-Induced Nephropathy %D 2020 %J Journal of Basic and Clinical Health Sciences %P -2564-7288 %V 4 %N 3 %R doi: 10.30621/jbachs.2020.1057 %U 10.30621/jbachs.2020.1057
ISNAD Manisaligil, Yasar Aysun , Cilaker Micili, Serap , Kizildag, Servet , Celik, Aslı , Bagriyanik, Husnu Alper , Gumustekin, Mukaddes . "Assessment of Rac1 and β-PAK Expressions in a Mouse Model for Contrast-Induced Nephropathy". Journal of Basic and Clinical Health Sciences 4 / 3 (Eylül 2020): 230-236 . https://doi.org/10.30621/jbachs.2020.1057
AMA Manisaligil Y , Cilaker Micili S , Kizildag S , Celik A , Bagriyanik H , Gumustekin M . Assessment of Rac1 and β-PAK Expressions in a Mouse Model for Contrast-Induced Nephropathy. JBACHS. 2020; 4(3): 230-236.
Vancouver Manisaligil Y , Cilaker Micili S , Kizildag S , Celik A , Bagriyanik H , Gumustekin M . Assessment of Rac1 and β-PAK Expressions in a Mouse Model for Contrast-Induced Nephropathy. Journal of Basic and Clinical Health Sciences. 2020; 4(3): 230-236.
IEEE Y. Manisaligil , S. Cilaker Micili , S. Kizildag , A. Celik , H. Bagriyanik ve M. Gumustekin , "Assessment of Rac1 and β-PAK Expressions in a Mouse Model for Contrast-Induced Nephropathy", Journal of Basic and Clinical Health Sciences, c. 4, sayı. 3, ss. 230-236, Eyl. 2020, doi:10.30621/jbachs.2020.1057