Araştırma Makalesi
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Yıl 2023, Cilt: 7 Sayı: 1, 507 - 515, 31.01.2023
https://doi.org/10.30621/jbachs.1210789

Öz

Kaynakça

  • Friedenstein A.j, Gorskaja J.F and Kulagina N.N. Fibroblast precursors in normal and irradiated mouse hematopoietic organs. Exp hematol 1976; (4): 267-74.
  • Jiang Y., Jahagirdar B.N, Reinhardt R.L, et al. pluripotency of mesenchymal stem cells derived from adult marrow. Nature 2002; 418-419.
  • İnan S, Özbilgin K. Kök hücre biyolojisi. Sağlıkta Birikim Dergisi 2008; 1(5): 11-24.
  • Keating A. Mesenchymal stromal cells. Current Opinion in Hematology 2006; (13): 419-25.
  • Crisan M, Yap S, Casteilla L, et al. Perivascular origin for mesenchymal stem cells in multiple human organs. Cell Stem Cell. 2008; (3): 301–313.
  • Pakdemirli A, Toksöz F, Karadağ A, et al. Role of mesenchymal stem cell-derived soluble factors and folic acid in wound healing. Turk J Med Sci 2019; (49): 914-921.
  • Avcu F. Mezenkimal kök hücrelerin ımmunregulatuar fonksiyonları. THD, MKHK. 2008; 14-16.
  • Krampera M, Pizzolo G, Aprilli G, et al. Mesenchymal stem cells for bone, cartilage, tendon and skeletal muscle repair. Bone 2006; (39): 678-83.
  • Alison M.R, Poulsom R. Hepatocytes from non-hepatic adult stem cells. Nature. 2000; (406):257.
  • Theise N.D, Nımmakayalu M. Gardner R, et al. Liver from bone marrow in humans. Hepatology 2000, (32): 11-16.
  • Ural AU. Mezenkimal Kök Hücre Uygulamaları (Yetişkin). Türkiye Klinikleri J Hem Onc 2008; (1); 62-65.
  • Özpek AÖ, Altıntaş L. Homeopatik Remediler, Veteriner Farmakoloji ve Toksikoloji Derneği Bülteni8 Bulletin of Veterinary Pharmacology and Toxicology Association. 2019; 10 (1): 31-39.
  • Schepper L. Hahnemann revisited: a textbook of classical homeopathy for the professional. 1st ed. Uttar Paradesh: B. Jain Publishers; 2001.
  • Vıthoulkas G. The Science of Homeopathy. 1st ed. New York: Grove Press; 2019.
  • Sankara R. Remedilerin Ruhu. İstanbul: Alperen Kitabevi&Sahaf; 2014.
  • Chandran PK, Kuttan R. Effect of calendula offi cinalis fl ower extract on acute phase proteins, antioxidant defense mechanism and granuloma formation during thermal burns. J Clin Biochem Nutr. 2008; 43(2):58- 64. Dikmen M, Öztürk Y, Sagratini G. Evaluation of the wound healing potentials of two subspecies of Hypericum perforatum on cultured NIH3T3 fibroblasts. Phytotherapy Res 2011; (25): 208-214.
  • Hostanska K, Rostock M, Melzer J, et al. A homeopathic remedy from arnica, marigold, St. John’s Wort and comfrey accelerates in vitro wound scratch closure of NIH3T3 fibroblasts. BMC Complementary and Alternative Medicine 2012; (12): 100-109.
  • Kameyama S, Shinho M, editors. Wound healing compositions from Aloe arborescens extracts. Japanese patent 79,151,113. Chemical Abstracts; 1980.
  • Arvidson K, Abdallah BM, Applegate LA, et al. Bone regeneration and stem cells. J Cell Mol Med. 2011;15(4):718–46.
  • World Health Organization International Agency for Research on Cancer. IARC Monographs on the Evaluation of Carcinogenic Risks to Humans. Volume 82 Some traditional herbal medicines, some mycotoxins, naphthalene and styrene. Lyon, France : IARC Press; 2002. pp. 43–45.
  • Tsintzas D, Vithoulkas G. Fracture treatment with the aid of the homeopathic remedy Symphytum officinale. A report of four cases. Clin Case Rep Rev. 2016; 2(5):422–4.
  • Jamil M, Mansoor M, Latif N, et al. Review effect of Aloe vera on Wound Healing. Pak. j. sci. ind. res. Ser. B: biol. sci 2020; 63(1):48-61.
  • Heggers JP, Kucukcelebi A, Listengarten D, et al. Beneficial effect of Aloe on wound healing in an excisional wound model. Journal of Integrative and Complementary Medicine 1996; 2(2): 271-7.
  • Sharrif Moghaddasi M. Aloe vera their chemicals composition and applications: A review. Res MJIJBMR 2011; 2(1): 466-71.
  • Chithra P, Sajithlal G, Chandrakasan G. Influence of Aloe vera on the glycosaminoglycans in the matrix of healing dermal wounds in rats. Journal of Ethnopharmacology 1998; 59 (3): 179-86.
  • Koparal, AT, Bostancıoğlu RB. Promotion of hair growth by traditionally used delphinium staphisagria seeds through ınduction of angiogenesis. ıranian journal of pharmaceutical research. IJPR 2016; 15(2): 551–560.
  • Izgu N. Complementary therapies in the management of ınduced oral mucositis during cancer treatment. Journal of Education and Research in Nursing 2017; 14(4): 304-311.
  • Akkol EK, Süntar I, Keleş H, et al. Bioassay-guided isolation and characterization of wound healer compounds from Morus nigra L. (Moraceae). Rec. Nat. Prod. 2015; 9(4):484-495.
  • Süntar I.P., Akkol E.K., Yılmazer D., et al. Investigations on the in vivo wound healing potential of Hypericum perforatum L. J. Ethnopharmacol. 2010; 127(2): 468-477.
  • Öztürk N., Korkmaz S., Öztürk Y. Wound healing activity of St. John’s Wort (Hypericum perforatum) on chicken embryonic fibroblasts. J. Ethanopharmacol 2007; 111: 33-39.
  • Prisacaru A.I., Andritoiu C., Andriescu C. Evaluation of the wound-healing effect of a novel Hypericum perforatum ointment in skin injury. J Morphol Embryol. 2013; 54(4):1053-9.
  • Sayar H, Gergerlioglu N, Seringec N, et al. Comparison of efficacy of topical phenytoin with hypericin in second-degree burn wound healing: an experimental study in rats. Med. Sci. Monit. Basic Res., 2014; 3 (20): 36-46.
  • Kıyan S, Uyanıkgil Y, Altuncı Y.A, et al. Investigation of acute effects of Hypericum perforatum (St. John’s Wort-Kantaron) treatment in experimental thermal burns and comparison with silver sulfadiazine treatment. Ulus Travma Acil Cerrahi Derg 2015; (21): 323-336.
  • Mainetti S., Carnevalli F. An experience with paediatric burnwounds treated with a plant-derived wound therapeutic. J Wound Care 2013; (22): 681-689.
  • Wölfle U, Seelinger G, Schempp C. M. (2014): Topical application of St. John’s wort (Hypericumperforatum). Planta medica 80(2-3): 109-120.

Effects of Homeopathic Remedies on Mesenchymal Stem Cell Proliferation: A Methodology Study

Yıl 2023, Cilt: 7 Sayı: 1, 507 - 515, 31.01.2023
https://doi.org/10.30621/jbachs.1210789

Öz

Background and purpose: Mesenchymal stem cells are multipotent cells of mesodermal origin,
which have the ability to differentiate into other cells in the bone marrow microenvironment under
suitable conditions in vitro. Homeopathy is a holistic practice method that aims to improve health
status with personalized homeopathic medicines. At the same time, it is among the certified training
programs of the Traditional and Complementary Medicine Practices of the Ministry of Health. Studies
evaluating the effect of homeopathic remedies in the field of stem cells are rarely encountered in the
literature. As a matter of fact, the material and method parts of these studies are not clear enough. In
our study, the methodology of working with mesenchymal stem cells of homeopathic remedies has
been presented in detail and it has been aimed to observe their proliferative effects.

Material and Methods: Mesenchymal stem cells were exposed to 10%, 20%, 50% and 100% ratios
of homeopathic remedies (30°C potency: Aloe, Calendula, Hypericum, Staphysagria) for 24 and 48
hours. The percent viability of cells at 24 and 48 hours was evaluated with the MTT (3-4,5-dimethyl-
thiazolyl-2,5-diphenyltetrazolium bromide) test.

Results: Aloe vera C 30 – Staphysagria C30 – Calencula C30 – Hypericum C30 remedies were found
to have an effect on mesenchymal stem cell proliferation at all doses, and the results were statistically
more significant when maintained in 10% media.

Conclusion: It is thought that the methodology used in this study on working methods with
homeopathic remedies will contribute to the literature and many scientific studies to be done in the
future.

Kaynakça

  • Friedenstein A.j, Gorskaja J.F and Kulagina N.N. Fibroblast precursors in normal and irradiated mouse hematopoietic organs. Exp hematol 1976; (4): 267-74.
  • Jiang Y., Jahagirdar B.N, Reinhardt R.L, et al. pluripotency of mesenchymal stem cells derived from adult marrow. Nature 2002; 418-419.
  • İnan S, Özbilgin K. Kök hücre biyolojisi. Sağlıkta Birikim Dergisi 2008; 1(5): 11-24.
  • Keating A. Mesenchymal stromal cells. Current Opinion in Hematology 2006; (13): 419-25.
  • Crisan M, Yap S, Casteilla L, et al. Perivascular origin for mesenchymal stem cells in multiple human organs. Cell Stem Cell. 2008; (3): 301–313.
  • Pakdemirli A, Toksöz F, Karadağ A, et al. Role of mesenchymal stem cell-derived soluble factors and folic acid in wound healing. Turk J Med Sci 2019; (49): 914-921.
  • Avcu F. Mezenkimal kök hücrelerin ımmunregulatuar fonksiyonları. THD, MKHK. 2008; 14-16.
  • Krampera M, Pizzolo G, Aprilli G, et al. Mesenchymal stem cells for bone, cartilage, tendon and skeletal muscle repair. Bone 2006; (39): 678-83.
  • Alison M.R, Poulsom R. Hepatocytes from non-hepatic adult stem cells. Nature. 2000; (406):257.
  • Theise N.D, Nımmakayalu M. Gardner R, et al. Liver from bone marrow in humans. Hepatology 2000, (32): 11-16.
  • Ural AU. Mezenkimal Kök Hücre Uygulamaları (Yetişkin). Türkiye Klinikleri J Hem Onc 2008; (1); 62-65.
  • Özpek AÖ, Altıntaş L. Homeopatik Remediler, Veteriner Farmakoloji ve Toksikoloji Derneği Bülteni8 Bulletin of Veterinary Pharmacology and Toxicology Association. 2019; 10 (1): 31-39.
  • Schepper L. Hahnemann revisited: a textbook of classical homeopathy for the professional. 1st ed. Uttar Paradesh: B. Jain Publishers; 2001.
  • Vıthoulkas G. The Science of Homeopathy. 1st ed. New York: Grove Press; 2019.
  • Sankara R. Remedilerin Ruhu. İstanbul: Alperen Kitabevi&Sahaf; 2014.
  • Chandran PK, Kuttan R. Effect of calendula offi cinalis fl ower extract on acute phase proteins, antioxidant defense mechanism and granuloma formation during thermal burns. J Clin Biochem Nutr. 2008; 43(2):58- 64. Dikmen M, Öztürk Y, Sagratini G. Evaluation of the wound healing potentials of two subspecies of Hypericum perforatum on cultured NIH3T3 fibroblasts. Phytotherapy Res 2011; (25): 208-214.
  • Hostanska K, Rostock M, Melzer J, et al. A homeopathic remedy from arnica, marigold, St. John’s Wort and comfrey accelerates in vitro wound scratch closure of NIH3T3 fibroblasts. BMC Complementary and Alternative Medicine 2012; (12): 100-109.
  • Kameyama S, Shinho M, editors. Wound healing compositions from Aloe arborescens extracts. Japanese patent 79,151,113. Chemical Abstracts; 1980.
  • Arvidson K, Abdallah BM, Applegate LA, et al. Bone regeneration and stem cells. J Cell Mol Med. 2011;15(4):718–46.
  • World Health Organization International Agency for Research on Cancer. IARC Monographs on the Evaluation of Carcinogenic Risks to Humans. Volume 82 Some traditional herbal medicines, some mycotoxins, naphthalene and styrene. Lyon, France : IARC Press; 2002. pp. 43–45.
  • Tsintzas D, Vithoulkas G. Fracture treatment with the aid of the homeopathic remedy Symphytum officinale. A report of four cases. Clin Case Rep Rev. 2016; 2(5):422–4.
  • Jamil M, Mansoor M, Latif N, et al. Review effect of Aloe vera on Wound Healing. Pak. j. sci. ind. res. Ser. B: biol. sci 2020; 63(1):48-61.
  • Heggers JP, Kucukcelebi A, Listengarten D, et al. Beneficial effect of Aloe on wound healing in an excisional wound model. Journal of Integrative and Complementary Medicine 1996; 2(2): 271-7.
  • Sharrif Moghaddasi M. Aloe vera their chemicals composition and applications: A review. Res MJIJBMR 2011; 2(1): 466-71.
  • Chithra P, Sajithlal G, Chandrakasan G. Influence of Aloe vera on the glycosaminoglycans in the matrix of healing dermal wounds in rats. Journal of Ethnopharmacology 1998; 59 (3): 179-86.
  • Koparal, AT, Bostancıoğlu RB. Promotion of hair growth by traditionally used delphinium staphisagria seeds through ınduction of angiogenesis. ıranian journal of pharmaceutical research. IJPR 2016; 15(2): 551–560.
  • Izgu N. Complementary therapies in the management of ınduced oral mucositis during cancer treatment. Journal of Education and Research in Nursing 2017; 14(4): 304-311.
  • Akkol EK, Süntar I, Keleş H, et al. Bioassay-guided isolation and characterization of wound healer compounds from Morus nigra L. (Moraceae). Rec. Nat. Prod. 2015; 9(4):484-495.
  • Süntar I.P., Akkol E.K., Yılmazer D., et al. Investigations on the in vivo wound healing potential of Hypericum perforatum L. J. Ethnopharmacol. 2010; 127(2): 468-477.
  • Öztürk N., Korkmaz S., Öztürk Y. Wound healing activity of St. John’s Wort (Hypericum perforatum) on chicken embryonic fibroblasts. J. Ethanopharmacol 2007; 111: 33-39.
  • Prisacaru A.I., Andritoiu C., Andriescu C. Evaluation of the wound-healing effect of a novel Hypericum perforatum ointment in skin injury. J Morphol Embryol. 2013; 54(4):1053-9.
  • Sayar H, Gergerlioglu N, Seringec N, et al. Comparison of efficacy of topical phenytoin with hypericin in second-degree burn wound healing: an experimental study in rats. Med. Sci. Monit. Basic Res., 2014; 3 (20): 36-46.
  • Kıyan S, Uyanıkgil Y, Altuncı Y.A, et al. Investigation of acute effects of Hypericum perforatum (St. John’s Wort-Kantaron) treatment in experimental thermal burns and comparison with silver sulfadiazine treatment. Ulus Travma Acil Cerrahi Derg 2015; (21): 323-336.
  • Mainetti S., Carnevalli F. An experience with paediatric burnwounds treated with a plant-derived wound therapeutic. J Wound Care 2013; (22): 681-689.
  • Wölfle U, Seelinger G, Schempp C. M. (2014): Topical application of St. John’s wort (Hypericumperforatum). Planta medica 80(2-3): 109-120.
Toplam 35 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Sağlık Kurumları Yönetimi
Bölüm Research Article
Yazarlar

Ahu Pakdemirli 0000-0001-9224-3007

Meral Sarper 0000-0002-9741-0641

Elif Güler Kazancı 0000-0003-0910-1142

Yayımlanma Tarihi 31 Ocak 2023
Gönderilme Tarihi 27 Kasım 2022
Yayımlandığı Sayı Yıl 2023 Cilt: 7 Sayı: 1

Kaynak Göster

APA Pakdemirli, A., Sarper, M., & Kazancı, E. G. (2023). Effects of Homeopathic Remedies on Mesenchymal Stem Cell Proliferation: A Methodology Study. Journal of Basic and Clinical Health Sciences, 7(1), 507-515. https://doi.org/10.30621/jbachs.1210789
AMA Pakdemirli A, Sarper M, Kazancı EG. Effects of Homeopathic Remedies on Mesenchymal Stem Cell Proliferation: A Methodology Study. JBACHS. Ocak 2023;7(1):507-515. doi:10.30621/jbachs.1210789
Chicago Pakdemirli, Ahu, Meral Sarper, ve Elif Güler Kazancı. “Effects of Homeopathic Remedies on Mesenchymal Stem Cell Proliferation: A Methodology Study”. Journal of Basic and Clinical Health Sciences 7, sy. 1 (Ocak 2023): 507-15. https://doi.org/10.30621/jbachs.1210789.
EndNote Pakdemirli A, Sarper M, Kazancı EG (01 Ocak 2023) Effects of Homeopathic Remedies on Mesenchymal Stem Cell Proliferation: A Methodology Study. Journal of Basic and Clinical Health Sciences 7 1 507–515.
IEEE A. Pakdemirli, M. Sarper, ve E. G. Kazancı, “Effects of Homeopathic Remedies on Mesenchymal Stem Cell Proliferation: A Methodology Study”, JBACHS, c. 7, sy. 1, ss. 507–515, 2023, doi: 10.30621/jbachs.1210789.
ISNAD Pakdemirli, Ahu vd. “Effects of Homeopathic Remedies on Mesenchymal Stem Cell Proliferation: A Methodology Study”. Journal of Basic and Clinical Health Sciences 7/1 (Ocak 2023), 507-515. https://doi.org/10.30621/jbachs.1210789.
JAMA Pakdemirli A, Sarper M, Kazancı EG. Effects of Homeopathic Remedies on Mesenchymal Stem Cell Proliferation: A Methodology Study. JBACHS. 2023;7:507–515.
MLA Pakdemirli, Ahu vd. “Effects of Homeopathic Remedies on Mesenchymal Stem Cell Proliferation: A Methodology Study”. Journal of Basic and Clinical Health Sciences, c. 7, sy. 1, 2023, ss. 507-15, doi:10.30621/jbachs.1210789.
Vancouver Pakdemirli A, Sarper M, Kazancı EG. Effects of Homeopathic Remedies on Mesenchymal Stem Cell Proliferation: A Methodology Study. JBACHS. 2023;7(1):507-15.