Comparison of Effectiveness Ascorbic Acid and Magnesium on Renal Ischemia-Reperfusion Model in Rats

Year 2025, Volume: 9 Issue: 1, 10 - 22, 31.01.2025

Ural Can Ekmekçi , Deniz Özzeybek , Şule Özbilgin , Mücahit Özdemir , Tuğba Erkmen , Semra Koçtürk , Hatice Efsun Kolatan , Gökçen Bilici , Güven Erbil

Abstract

Project Number

2019.KB.SAG.019

Comparison of Effectiveness Ascorbic Acid and Magnesium on Renal Ischemia-Reperfusion Model in Rats

Abstract

ABSTRACT
Background: This study aims to determine the effectiveness of ascorbic acid and magnesium administration separately or combined before ischemia in the rat renal ischemia and reperfusion damage of the model.
Materials and methods: Thirty-five Wistar rats were randomized into five groups of seven animals each. Group1 only underwent laparotomy, followed for 285min until sacrification. Group2 45min ischemia; 240min reperfusions, no drugs were applied. Group 3 250mg/kg ascorbic acid was applied 60min before ischemia-reperfusion, Group4 200mg/kg magnesium sulfate was applied 60min before ischemia-reperfusion, Group5 both drugs were applied at the same doses 60min before ischemia-reperfusion. Malondialdehyde (MDA) and glutathione (GSH) levels were determined in the renal tissues received, serum blood urea nitrogen (BUN) and creatinine levels were measured in blood samples. H&E and PAS paintings for histological examinations. TUNEL immunohistochemical staining for apoptotic cell examinations.
Results: Oxidative stress parameter MDA value was found significantly lower in Group3 (p:0.034) and Group5 (p<0.001) compared to Group2. Endogen antioxidant parameter GSH value of Group 4 (p<0.001) and Group 5 (p<0,001) were significantly higher than the other groups. TUNEL counts of the Group5 was significantly lower than the countsGroup2 (p:0,005).
Conclusion: It was concluded that before ischemia, ascorbic acid and magnesium sulfate combination reduce the kidney damage in the rat renal IR model

Keywords

Kidney , Ischemia-Reperfusion Injury , Ascorbic Acid , Magnesium , Rat

Ethical Statement

This research was approved by Dokuz Eylul University Ethics Committee (no: 47/2018, decision date: 31.10.2018)

Supporting Institution

This work was supported by Dokuz Eylul University Scientific Research Coordinator (project number: 2019.KB.SAG.019).

Project Number

2019.KB.SAG.019

Thanks

None

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