Microbiological Monitoring of Olive Fermentation Process of Gemlik Type Dry-Salted Black Olives and Investigation of the Effect of Pasteurization on the Product’s Shelf Life

Sibel Alak; Vildan Uylaşer

Microbiological Monitoring of Olive Fermentation Process of Gemlik Type Dry-Salted Black Olives and Investigation of the Effect of Pasteurization on the Product’s Shelf Life

Abstract

In the present research, the microbiological changes that occurred during the fermentation process of traditionally produced Gemlik style dry-salted olives, locally known as “sele”, were monitored. Following the fermentation process, microbiological, chemical and organoleptic changes were investigated for the products which were stored at room temperature for a period of 6 months after various pasteurization processes (non-brined, oiled and non-oiled). At the initial stages of fermentation, microbial flora consisted of molds and lactic acid bacteria, and in the following stages no presence of lactic acid bacteria could be detected. None of Gemlik style dry-salted (sele) olive samples contained coliform bacteria. During the storage period, the highest total bacteria count (1,2x105 cfu/g) was detected in the control-oiled group and the lowest total count (10 cfu/g) was observed in the pasteurized non-oiled group. The samples within the control group exhibited mold growth, whereas the pasteurized samples rarely exhibited yeast/mold growth. Throughout the storage period, dry matter, pH, total acidity, protein, oil content and oleuropein (absorbance) values of samples were monitored, and no significant change was observed among the sample groups. In general, all samples were relished according to the organoleptic evaluation results conducted after fermentation and throughout the storage period. However, with their attractive appearance, oiled group Gemlik style dry-salted (sele) olives were preferred at higher rates compared to non-oiled samples with dull appearance. Additionally, the slightly rancid taste of oiled group samples towards the end of the storage period, received adverse opinions of some of the panelists. Saltiness values, on the other hand, were found to be at normal levels.

Keywords

Olive, Dry curing, Pasteurization, Shelf life

References

Alves M, Gonçalves T, Quintas C (2012). Microbial quality and yeast population dynamics in cracked green table olives’ fermentations. Food Control 23: 363-368.
Anonymous (2005a). Toplam bakteri sayımı: Merck gıda mikrobiyolojisi uygulamaları (Ed: AK Halkman). Başak Matbaacılık Ltd. Şti., Ankara, s: 135-140.
Anonymous (2005b). Koliform grup bakteriler: Merck gıda mikrobiyolojisi uygulamaları (Ed: AK Halkman). Başak Matbaacılık Ltd. Şti., Ankara, s: 142-149.
Anonymous (2014). Türk Gıda Kodeksi, Sofralık Zeytin Standardı. Resmi Gazete, Sayı: 29097.
Arroyo-López FN, Querol A, Bautista-Gallego J and Garrido-Fernández A (2008). Role of yeasts in table olive production. International Journal of Food Microbiology 128: 189-196.
Asehraou A, Faid M and Jana M (1992). Physicochemical properties and the microflora of Moroccan black table olives. Grasas y Aceites 43: 130-133.
Ayhan K ve Ergen KÖ (2006). Sofralık Zeytinlerde Biyojen Amin Miktarlarının Belirlenmesi. Ankara Üniversitesi Bilimsel Araştırma Projeleri 20050745003HPD nolu Proje Kesin Raporu, Ankara.
Balatsouras G (1990). Edible olive cultivars, chemical composition of fruit, harvesting, transportation, processing, sorting and packaging, styles of black olives, deterioration, quality standards, chemical analysis, nutritional and biological value of the end product. In: Olio d’oliva e olive da tavola: tecnologia e qualita. Istituto Sperimentale per la Elaiotecnica, 25–28 April, Pescara-Italy, pp. 291–330.
Balatsouras GD (1966). The chemical composition of the brine of stored Greek black olives. Grasas y Aceites 17: 83-88.
Balatsouras GD (2004). Table olives: Cultivars, chemical composition, commercial preparations, quality standards, packaging, marketing. Agricultural University of Athens, Greek.

Bautista-Gallego J, Rodriguez-Gomez F, Barrio E, Querol A, Gorrido-Fernandez A, Arroyo-Lopez FN (2011). Exploring the yeast biodiversity of green table industrial fermentations for technological applications. International Journal of Food Microbiology 32: 87-96.
Bianchi G (2003). Lipids and phenols in table olives. European Journal of Lipid Science and Technology 105: 229-242.
Blazquez Martınez JM (1996). Evolution and history. In: World Olive Encyclopedia, International Olive Oil Council (IOOC), (Eds.: IOOC). Madrid-Spain, pp. 17-54.
Borcaklı M, Özay G, Alperden I, Ozsan E and Erdek Y (2000). Changes in the chemical and microbiological composition of two varieties of olive during fermentation. Grasas y Aceites 44: 253-260.
Cardoso SM, Mafra I, Reis A, Georget D, Smith AC, Waldron KW and Coimbra MA (2008). Effect of dry-salt processing on the textural properties and cell wall polysaccharides of cv. Thasos black olives. Journal of Science Food Agriculture 88: 2079-2086.
Cemeroğlu B (1992). Meyve ve sebze işleme endüstrisinde temel analiz metotları. Biltav Üniversite Kitapları Serisi No:02-2, Ankara, 381 s.
Cemeroğlu B, Yemencioğlu A ve Özkan M (2001). Meyve ve sebze işleme teknolojisi. Gıda Teknolojisi Dergisi Yayınları No: 24, Ankara, 328s.
Duran Quintana MC, Garcia Garcia P and Garrido Fernadez A (1999). Establishment of conditions for green table olive fermentation at low temperature. International Journal of Food Microbiology 51: 133-143.
Garcia E, Luh BS and Martin MH (2005). Olives. In: Processing Fruits Science an Technology, (Eds: M.B. Diane, P.S. Laszlo and H.S. Ramaswamy) CRC Press LLC.
Garrido-Fernández A, Fernández-Díez MJ and Adams MR (1997). Table olives, production and processing. Chapman and Hall, London, 495 p.
Gür E, Aslan R, Son L, Pala H and Nas S (2011). Mut yöresinde organik zeytin yetiştiriciliği. T.C. Tarım ve Köyişleri Bakanlığı, Tarımsal Araştırmalar Genel Müdürlüğü, Ankara/Turkey, http://orgprints.org/19283 (Erişim tarihi: 16.07.2017).
Hutkins RW (2006). Microbiology and technology of fermented foods. Blackwell Publishing, London, 457 p.
Idoui T and Bouchefra A (20014). The black olive fruits of Jijelian Sigoise Variety (Eastern Algeria): quality evaluation for possible use as table olives and pesticides research. The Online Journal of Science and Technology-January 4(1): 45-52.
IOOC (2017). International Olive Oil Council. Available from http://www.internationaloliveoil.org/noticias. Accessed Eylül 25, 2017.
Kailis S and Harris D (2007). Table olive processing: general aspects. In: Producing table olives. CSIRO publishing, Landlinks Press, Collingwood-Australia, 131-189 pp.
Keçeli T and Gordon MH (2001). The antioxidant activity and stability of phenolic fraction in green olives and extra virgin olive oil. Journal of The Science of Food and Agriculture 81(14): 1391-1396.
Kılıç O (1994). Zeytin yetiştiriciliği ve sofralık zeytin üretimi. Marmarabirlik Yayınları No:2, Bursa, 62 s.
Korukluoğlu M, Gürbüz O and Şahin İ (2002). Taze zeytin mikroflorasında bulunan laktik asit bakterilerinin belirlenmesi. Tarım Bilimleri Dergisi 8: 109-113.
Mastorakis M, Sotiroudis TG, Xenakis A and Miniadismeimaroglou S (2004). Spectrophotometric analysis of enzymic and nonenzymic oxidation of oleuropein. Chemistry and Physics of Lipids 130: 58.
Mucilli S, Caggia C, Randazzo CL and Restuccia C (2011). Yeast dynamics during the fermentation of brined green olives treated in the field with kaolin and Bordeaux mixture to control the olive fruits fly. International Journal of Food Microbiology 108: 396-405.
Özay G and Borcaklı M (1996). Effect of brine replacement and salt concentration on the fermentation of naturally black olives. Food Research International 28: 553-559.
Özdemir Y, Öztürk A, Güven E, Özkan M and Kurultay K (2013). Sofralık zeytinde mikotoksin riski. Gıda&Yem Analiz 35 19: 42-48.
Özer K, Tetik D, Baysal T, Öngen G and Sarıgül N (2003). Hurma, sele ve naturel siyah zeytinlerin cam kavanoz içinde salamurasız olarak muhafaza imkanlarının araştırılması. TC Gıda, Tarım ve Hayvancılık Bakanlığı, Zeytincilik Araştırma Enstitüsü Sonuçlanmış Projeler Yayın No: 118.
Panagou EZ (2006). Greek dry-salted olives: Monitoring the dry-salting process and subsequent physico-chemical and microbiological profile during storage under different packing conditions at 4 and 20 °C. LWT 39: 322-329.
Panagou EZ and Katsaboxakis CZ (2006). Effect of different brining treatments on the fermentation of cv. Conservolea green olives processed by the Spanishmethod. Food Microbiology 23: 199-204.
Panagou EZ, Tassou CC and Katsaboxakis CZ (2002). Microbiological, physicochemical and organoleptic changes in dry-salted olives of Thassos variety stored under different modified atmospheres at 4 and 20 °C. International Journal of Food Science and Technology 37: 635-641.
Papagora C, Roukas T and Kotzekidou P (2013). Optimization of extracellular lipase production by Debaryomyces hansenii isolates from dry-salted olives using response surface methodology. Food and Bioproducts Processing 91(4): 413-420.
Rejano L, Montano A, Casado FJ, Sanchez AH and Castro A (2010). Table olives; varieties and variations. In: The plant, production, olives and olive oil and their detailed characterization, (Eds: P.Victor and W. Ronald). Elsevier, Amsterdam, pp. 45-50.
Romeo FV (2012). Microbiological aspects of table olives. Agricultural Research Council - Olive Growing and Oil Industry Research Centre, Rende (CS)-Italy, 15: 321-324.
Romeo FV and Poiana M (2007). Ability of commercially available Lactobacillus strains as starters in brining and debittering of table olives. Acta Alimentaria 36(1): 49-60.
Ruiz-Barbara JL, Cathcart DP, Warner PJ and Jimenz-Diaz R (1994). Use of Lactobacillus plantarum LPCO10, a bacteriocin producer, as a starter culture in Spanish-style green olive fermentations. Applied and Environmental Microbiology 60: 2059-2064.
Sanchez JC, Alsina MA, Herrlein MK and Mestres C (2007). Interaction between the antibacterial compound, oleuropein, and model membranes. Colloid and Polymer Science 285:1351-1360.
Savaş E and Uylaser V (2013). Quality improvement of green table olive cv. ‘Domat’ (Olea europaea L.) grown in Turkey using different de-bittering methods. Not Bot Horti Agrobo 41(1): 269-275.
Soler-Rivas C, Espin JC and Wichers HJ (2000). Oleuropein and related compounds. Journal of The Science of Food and Agriculture 80: 1013-1023.
Şahin İ, Korukluoğlu M, Uylaşer V ve Göçmen D (2000). Diyet Zeytini ve Zeytin Ezmesi Üretimi. Türkiye 1. Zeytincilik Sempozyumu, 6-9 Haziran, Bursa.
Tanılgan K, Özcan MM and Ünver A (2007). Physical and chemical characteristics of five Turkish olive (Olea europea L.) varieties and their oils. Grasas y Aceites 58: 142-147.
Tantaoui-Elaraki A, Samane S and Roquebert MF (1990). Mycoflora of Moroccan ‘‘Greek-style’’ black olives. Microbiologie-Aliments-Nutrition 8: 257-264.
Toker C and Aksoy U (2013). Kuzey Ege agroekolojik şartlarında yetişen Ayvalık çeşidi zeytin meyvesinin kalite özellikleri. Gıda Teknolojileri Elektronik Dergisi 8(3): 51-57.
Tokuşoğlu Ö (2010). Özel Meyve Zeytin: Kimyası, Kalite ve Teknolojisi. Sidas Medya, Manisa, 330 s. Tuna S and Akpınar-Bayızıt A (2009). The use of β-glucosidase enzyme in black table olives fermentation. Not Bot Horti Agrobo 37: 182-189.
Tunalıoğlu R (2002). Zeytinyağı. TEAE Bakış Raporu. Tarımsal Ekonomi Araştırma Enstitüsü, Ankara.
Tzika E, Papadimitriou V, Sotiroudis TG and Xenakis A (2004). Chemical and enzymatic oxidation of oleuropein: an EPR study. Chemistry and Physics of Lipids 130: 61.
Uccella N (2001). Olive biophenols: novel ethnic and technological approach. Trends in Food Science and Technology 11: 328-339.
Uylaşer V (2015). Changes in phenolic compounds during ripening in Gemlik variety olive fruits obtained from different locations. CyTA-Journal of Food 13(2): 167-173.
Uylaşer V and Başoğlu F (2011). Temel Gıda Analizleri. Dora Yayıncılık, Bursa, 125 s.
Uylaşer V, Korukluoğlu M, Göçmen D, Yıldırım A and Şahin İ (2000). Yeşil zeytin üretiminde farklı çeşit ve uygulamaların ürün kalitesine etkisi. Türkiye 1. Zeytincilik Sempozyumu, 6-9 Haziran, Bursa.
Uylaşer V, Tamer CE, Incedayı B, Vural H and Copur OU (2008). The quantitative analysis of some quality criteria of Gemlik variety olives. J. Food, Agric. Environ. 6(3–4): 26–30.
Uylaser V and Yıldız G (2014). The historical development and nutritional importance of olive and olive oil constituted an important part of the mediterranean diet. Critical Reviews in Food Science and Nutrition 54: 1092–1101.
Viljoen BC (2006). Yeast ecological interactions, yeast–yeast, yeast bacteria, yeast–fungi interactions and yeasts as biocontrol agents. In: Yeasts in Food and Beverages (Eds: A. Querol and H. Fleet). Springer-Verlag, Berlin, pp. 83-110.

Details

Primary Language

English

Subjects

Agricultural Biotechnology (Other)

Journal Section

Research Article

Authors

Sibel Alak This is me
Türkiye

Vildan Uylaşer ^*
Türkiye

Publication Date

December 28, 2017

Submission Date

October 30, 2017

Acceptance Date

November 17, 2017

Published in Issue

Year 2017 Volume: 11 Number: 33

IZ

https://izlik.org/JA38NF32RM

APA

Alak, S., & Uylaşer, V. (2017). Microbiological Monitoring of Olive Fermentation Process of Gemlik Type Dry-Salted Black Olives and Investigation of the Effect of Pasteurization on the Product’s Shelf Life. Journal of Biological and Environmental Sciences, 11(33), 149-164. https://izlik.org/JA38NF32RM

AMA

1.Alak S, Uylaşer V. Microbiological Monitoring of Olive Fermentation Process of Gemlik Type Dry-Salted Black Olives and Investigation of the Effect of Pasteurization on the Product’s Shelf Life. JBES. 2017;11(33):149-164. https://izlik.org/JA38NF32RM

Chicago

Alak, Sibel, and Vildan Uylaşer. 2017. “Microbiological Monitoring of Olive Fermentation Process of Gemlik Type Dry-Salted Black Olives and Investigation of the Effect of Pasteurization on the Product’s Shelf Life”. Journal of Biological and Environmental Sciences 11 (33): 149-64. https://izlik.org/JA38NF32RM.

EndNote

Alak S, Uylaşer V (December 1, 2017) Microbiological Monitoring of Olive Fermentation Process of Gemlik Type Dry-Salted Black Olives and Investigation of the Effect of Pasteurization on the Product’s Shelf Life. Journal of Biological and Environmental Sciences 11 33 149–164.

IEEE

[1]S. Alak and V. Uylaşer, “Microbiological Monitoring of Olive Fermentation Process of Gemlik Type Dry-Salted Black Olives and Investigation of the Effect of Pasteurization on the Product’s Shelf Life”, JBES, vol. 11, no. 33, pp. 149–164, Dec. 2017, [Online]. Available: https://izlik.org/JA38NF32RM

ISNAD

Alak, Sibel - Uylaşer, Vildan. “Microbiological Monitoring of Olive Fermentation Process of Gemlik Type Dry-Salted Black Olives and Investigation of the Effect of Pasteurization on the Product’s Shelf Life”. Journal of Biological and Environmental Sciences 11/33 (December 1, 2017): 149-164. https://izlik.org/JA38NF32RM.

JAMA

1.Alak S, Uylaşer V. Microbiological Monitoring of Olive Fermentation Process of Gemlik Type Dry-Salted Black Olives and Investigation of the Effect of Pasteurization on the Product’s Shelf Life. JBES. 2017;11:149–164.

MLA

Alak, Sibel, and Vildan Uylaşer. “Microbiological Monitoring of Olive Fermentation Process of Gemlik Type Dry-Salted Black Olives and Investigation of the Effect of Pasteurization on the Product’s Shelf Life”. Journal of Biological and Environmental Sciences, vol. 11, no. 33, Dec. 2017, pp. 149-64, https://izlik.org/JA38NF32RM.

Vancouver

1.Sibel Alak, Vildan Uylaşer. Microbiological Monitoring of Olive Fermentation Process of Gemlik Type Dry-Salted Black Olives and Investigation of the Effect of Pasteurization on the Product’s Shelf Life. JBES [Internet]. 2017 Dec. 1;11(33):149-64. Available from: https://izlik.org/JA38NF32RM

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