Chrysin protects neuronal cells against carboplatin exposure-induced apoptosis and oxidative damage

Abstract

Chemotherapy drugs such as carboplatin are commonly used to treat various cancers, including testicular, lung, and ovarian cancer. Although carboplatin primarily targets cancer cells, it can also damage healthy cells, including neurons, leading to potential adverse effects. Notably, some side effects of carboplatin therapy are associated with nerve cells and the nervous system. The aim of our study was to investigate the potential protective effects of chrysin (Chr) against carboplatin-induced toxicity in SH-SY5Y neuronal cells. In this study, the ameliorative effects of Chr on carboplatin-induced cellular toxicity were evaluated through cell viability assays, lipid peroxidation (LPO) analysis to assess antioxidant capacity, TUNEL assay, immunohistochemistry (IHC) staining, and western blotting to examine anti-apoptotic activities. The results indicated that Chr mitigates carboplatin toxicity in SH-SY5Y cells by reducing LPO levels and the expression of cytochrome c (Cyt c) and Bax, while increasing the expression of the anti-apoptotic protein Bcl-2. The study also demonstrated that carboplatin caused apoptosis by causing DNA strand breaks while Chr treatment alleviated these effects. These findings suggest that the use of antioxidants, particularly Chr, may diminish the apoptotic effects of carboplatin in SH-SY5Y cells and could provide insights into potential therapeutic strategies for mitigating cell damage caused by carboplatin.

Keywords

• Apoptosis
• Carboplatin
• chrysin
• SH-SY5Y cells
• neurotoxicity

Supporting Institution

Bingol University

Project Number

BAP-FEF.2021.009

Ethical Statement

No

Thanks

We are grateful to Bingöl University Department of Molecular Biology and Genetics Department for useful discussions and aloowing us to access laboratory equipments when needed.

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