Levels of leukocyte oxidative DNA damage (8-OHdG), serum coenzyme Q10 and lipid peroxidation in the formation attacks of patients with multiple sclerosis

Abstract

Multiple sclerosis (MS) is a demyelinating disease of the nervous system. Evidence about oxidative stress plays an important role in the pathogenesis of MS is increasing day by day. In our study, we aimed to investigate the effect of oxidative DNA damage and oxidative stress in the pathogenesis of MS disease. Blood samples were obtained from during an attack (Group 1), between attacks (Group 2) of MS patients (20 male and 10 female) and 30 healthy volunteers (Group 3). Malondialdehyde (MDA) levels as indicator of oxidized lipids were detected using fluorescence dedector with high pressure liquid chromatograph (HPLC). DNA was extracted from leukocytes of control and patients with MS and then we measured 8-hydroxy- 2' -deoxyguanosine (8-OHdG) and deoxyguanosin (dG) by using HPLC method with electrochemical and UV detector, respectively. Measurement of oxidized coenzyme Q10 (CoQ10) and reduced CoQ (CoQ10H) was performed by using UV detector with HPLC method. Serum MDA level of group 1 was significantly higher than those in group 2 and group 3 (p< 0.001). 8- OHdG/106 dG ratio of group 1 was significantly higher than those in group 2 and group 3 (p< 0.001). CoQ10/CoQ10H rates of group 1 were significantly increased compared with group 2 and group 3 (p<0.001). In conclusion, we observed that oxidative DNA damage, lipid and mitochondria oxidative damage were high in blood of patients with MS. It seems that oxidative stress acts a play role the pathogenesis of MS patients as well as induces attacks.

Keywords

• Coenzyme Q10; Lipid peroxidation; Oxidative DNA damage; Multiple sclerosis

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