Antioxidative and Neurotherapeutic Effect of Ascorbic acid on Albino rats Induced with Traumatic Brain Injury

Yıl 2020, , 922 - 936, 12.10.2020

Ibrahim Bulama , Nasiru Suleiman , Lawal Bilbis , Abdullahi Abbas , Nasiru Jinjiri , Yusuf Saidu , Mohamad Aris Mohd Moklas , Che Norma Mat Taib , Musa Chiroma

https://doi.org/10.37212/jcnos.800233

Öz

Generation of reactive oxygen species (ROS) during traumatic brain injury (TBI) has been identified as an important factor that is responsible for disease progression and cell death, particularly in secondary injury process. Ascorbic acid (AA) is an exogenous antioxidant that can be used to quench ROS in neurodegeneration. Its antioxidant properties have been reported in some neurodegenerative conditions in rats. In the present study, we examined the neurotherapeutic effects of AA in TBI induced rats. Three groups of seven rats each were used for this study. Group I was induced with TBI and treated with AA (67.5 mg/kg orally), group II was traumatized but not treated (TNT) while group III was neither traumatized nor treated (NTNT). Treatment started 30 min after TBI and lasted for 21 days. Morris water maze (MWM), Elevated plus maze, and Open field test were carried out. Antioxidant enzymes [(Superoxide dismutase (SOD), Catalase (CAT) Glutathion peroxidase (GPx)] and their gene expression were analyzed. Malondialdehyde level, S100B concentration and histological studies were conducted. The treatment with AA improved learning and memory, locomotor function and decreased anxiety in the treated groups compared to group II. S100B was significantly (p<0.05) lowered in the treated group compared to the group II rats. Treatment with AA also decreases malondialdehyde level when compared to group II. There were increased SOD, CAT and GPx activities in the treated group when compared to the control group. These were in agreement with their gene expressions that are highly expressed in the same groups. Our results suggest that neuroprotective effects of AA in albino rats can be the result of reduced lipid peroxidation and enhanced antioxidant status which can be a good neurotherapeutic strategy.

Anahtar Kelimeler

Ascorbic acid, , Neurotherapeutic, antioxidant, Neurological, Neurodegeneration, antioxidant,

Kaynakça

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