HIV pozitif hastalarda HHV-6 prevalansı ve varyant tiplerinin belirlenmesi

Abstract

Amaç: Bu çalışmanın amacı HIV enfeksiyonu pozitif saptanan hastalarda HHV-6 DNA sıklığının, HHV-6 DNA’sı pozitif saptanan olgularda ise HHV-6 varyant türünün (varyant A veya varyant B) belirlenmesiydi.

Gereç ve Yöntem: Çalışmaya 20 HIV pozitif hasta ile 57 HIV negatif sağlıklı birey dahil edildi. Hasta ve kontrol grubunda HHV-6 DNA’sı polimeraz zincir reaksiyonu (PZR) ile araştırıldı. HHV-6 DNA pozitif saptanan olgularda HHV-6 varyant türü restriksiyon parça uzunluğu polimorfizmi (restriction fragment lenght polymorphisim:  RFLP) ile araştırıldı. İstatistiksel değerlendirme SPSS programında yapıldı, p≤0.05 istatistiksel olarak anlamlı kabul edildi.

Bulgular: HIV pozitif hastalarda HHV-6 DNA pozitifliği %45 (9/20), HIV negatiflerde ise %33,3 (19/57) olarak belirlendi. Toplamda pozitiflik oranı %36,3 (28/77) idi. HIV pozitif hastalarda HHV-6 varyant B saptanırken, varyant A saptanmadı.

Sonuç: HIV pozitif hastalarda HHV-6 seropozitifliği ve varyant tiplerinin öneminin belirlenmesi için daha fazla sayıda olgu içeren başka klinik çalışmalara gereksinim olduğu görüşündeyiz.

Keywords

• HIV,HHV-6,varyant A,varyant B,koenfeksiyon

The prevalence of HHV-6 and variant type in HIV positive patients

Abstract

Objective: The aim of this study was to determine the frequency of HHV-6 DNA in patients with HIV positive infection and HHV-6 variant type (variant A or variant B) in patients with HHV-6 DNA positive

Material and Method: Twenty HIV-positive patients and 57 HIV negative healthy subjects were included in the study. HHV-6 DNA was investigated by polymerase chain reaction (PCR) in the patient and control groups. In cases with HHV-6 DNA positive, HHV-6 variant was investigated with restriction fragment length polymorphism (RFLP). Statistical analysis was performed using SPSS program, p≤0.05 was considered statistically significant.

Results: HHV-6 DNA positivity was found to be 45% (9/20) in HIV positive patients and 33.3% (19/57) in HIV negative patients. Overall, the positivity rate was 36.3% (28/77). HHV-6 variant B was detected in HIV positive patients, but variant A was not.

Conclusion: We believe that further clinical studies involving more cases are needed to determine the importance of HHV-6 seropositivity and variant types in HIV positive patients.

Keywords

• HIV,HHV-6,varyant A,varyant B,coinfection

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