Determination of genotoxic effects in vitro of ZnOTiO2 nanoparticles on human peripheral lymphocytes

Abstract

The aim of this study was to examine the genotoxic effects of ZnOTiO₂ NPs using chromosome aberration (CA) and micronucleus (MN) tests in human peripheral lymphocytes. Cells were treated to ZnOTiO₂ NPs concentrations in the range of 12.5 to 125 µg/mL for 24 and 48 hours. In addition, it induced structural CA at all concentrations with ZnOTiO₂ NPs treatment. However, nanoparticles did not stimulate CA in a dose-dependent manner for 24 and 48 hours of treatment, and there were no statistically significant differences between both the administration time and the concentrations (P > 0.05). The formation of MN induced by ZnOTiO₂ NPs increased depending on concentration and dose. In particular, the highest 2 concentrations (100 and 125 µg/ml) were observed to be significantly increased during the 24 and 48 hour treatment period (P > 0.05). Again, there is no significant difference in the occurrence of MN depending on gender (P > 0.05).

Keywords

• Chromosome aberration,ZnOTiO2 NPs,nanoparticles,nanotoxicology,micronucleus

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