Onobrychis argyrea subsp. argyrea Ekstrelerinin Antioksidan, Antimikrobiyal ve Antiproliferatif Aktivitelerinin Belirlenmesi

Öz

Fabaceae familyasına ait bitkiler, hayvan beslenmesinde ve insan sağlığının korunmasında (antidiyabetik, antikanserojen, antioksidan, antienflamatuar ve kardiyovasküler) büyük öneme sahiptir. Bu familyanın önemli cinslerinden biri olan Onobrychis yüksek fenolik içeriğe sahiptir ve güçlü bir antioksidan, antikanserojen ve antimikrobiyal ajan olarak bilinir. Bu çalışmada, Onobrychis argyrea subsp. argyrea (O. argyrea) türünün kloroform (OAC), etanol (OAE) ve su (OAW) ekstreleri hazırlanmış ve bu ekstrelerin antiproliferatif, antimikrobiyal ve antioksidan aktiviteleri incelenmiştir. Ekstraktların antiproliferatif etkileri farklı kanser hücre dizileri üzerinde XTT kolorimetrik yöntemi ile değerlendirilmiştir. Bulgulara göre, OAE ekstresi tüm hücre hatlarına karşı en yüksek etkinliği göstermiştir. MCF-7 %83.71, A549 %92.14 ve HT-29 %72.24 inhibe edildi. Tüm ekstrelerin Staphylococcus aureus, Escherichia coli ve Streptococcus pneumoniae'ye karşı antimikrobiyal aktivitesi, disk difüzyon tekniği kullanılarak belirlendi. Son olarak antioksidan gücü DPPH ve FRAP yöntemleri kullanılarak belirlendi. DPPH yönteminde, OAE eksrtesi en yüksek radikal süpürme aktivitesi gösterdi (IC50: 34.12±0.2). OAE (257.95±1.40) ve OAW (282.14±0.83) ekstreleri, FRAP yönteminde benzer sonuçlarda yüksek aktivite göstermiştir. Toplam fenolik madde içeriği OAE için 226.15±1.89 mg GAE/g ve OAW için 146.52±0.71 mg GAE/g olarak belirlendi. Bu sonuçlar, çalışılan bitkinin önemli bir antioksidan kaynağı olduğunu göstermektedir. Bütün bu sonuçlardan bitkinin çeşitli biyolojik aktivitelere ve zengin ikincil metabolitlere sahip olduğu anlaşılmaktadır.

Anahtar Kelimeler

• Onobrychis argyrea subsp. argyrea
• antioksidan
• antiproliferatif
• antimikrobial

Determination of Antioxidant, Antimicrobial, and Antiproliferative Activities of Onobrychis argyrea subsp. argyrea Extracts

Öz

Plants belonging to the Fabaceae family have great importance in animal nutrition and the protection of human health (antidiabetic, anticarcinogenic, antioxidant, anti-inflammatory, and cardiovascular). Onobrychis, known as a potent antioxidant, anticarcinogenic and antimicrobial agent, its high phenolic substance is one of the important genera of this family. In this study, chloroform (OAC), ethanol (OAE), and water (OAW) extract of Onobrychis argyrea subsp. argyrea (O. argyrea) specie was prepared; and the antiproliferative, antimicrobial, and antioxidant activities were investigated. The antiproliferative effects of the extracts were evaluated on different cancer cell lines by XTT colourimetric method. OAE demonstrated the most excellent efficacy against all cell lines, according to the findings. MCF-7 83.71%, A549 92.14%, and HT-29 72.24% were all inhibited. The antimicrobial activity of OAE and OAW extracts against Staphylococcus aureus, Escherichia coli, and Streptococcus pneumoniae was determined using the disc-diffusion technique. Finally, antioxidant power was determined using DPPH and FRAP methods. In the DPPH research, OAE and OAW extracts showed the highest levels of inhibition (IC50: 34.12±0.2 and 21.58±0.12, respectively). OAE (257.95±1.40) and OAW (282.14±0.83) extracts showed high activity with similar results in the FRAP method. Total phenolic content was determined as 226.15±1.89 mg GAE/g for OAE and 146.52±0.71 mg GAE/g for OAW, respectively. These results show that the species is an essential source of antioxidants. These studies are the first data for Onobrychis argyrea subsp. argyrea. All these results show that the plant has various biological activities and rich secondary metabolites.

Anahtar Kelimeler

• Onobrychis argyrea subsp. argyrea
• antioxidant
• antiproliferative
• antimicrobial

Kaynakça

1. Açikgöz, E., (1988). Annual forage legumes in the arid and semi-arid regions of Turkey. In Nitrogen fixation by legumes in Mediterranean agriculture (pp. 47-54). Springer, Dordrecht.
2. Aliahmadi, A., Roghanian, R., Emtiazi, G., & Ghassempour, A., (2011). A simple method for primary screening of antibacterial peptides in plant seeds. Iranian Journal of Microbiology 3(2), 104-108.
3. Batool, S., Javaid, S., Javed, H., Asim, L., Shahid, I., Khan, M., & Muhammad, A. (2022). Addressing artifacts of colourimetric anticancer assays for plant-based drug development. Medical Oncology, 39(12), 1-19.
4. Bauer, A.W., Kirby, W.M.M., Serris, J.C. & Turck, M., (1966). Antibiotic susceptibility testing by a standardized single disc method. American Journal of Clinical Pathology 45: 493-496.
5. Baytop, A., (1988). İstanbul eczacılık fakültesi herbaryumundaki türkiye bitkileri, İstanbul, s. 60-61.
6. Blois, M. S.,(1958). Antioxidant determinations by the use of a stable free radical. Nature, 181(4617), 1199-1200.
7. Bülbül, M. V., (2017). Bazı endemik onobrychis türlerinin kolon kanseri hücreleri üzerindeki etkilerinin incelenmesi (Master's thesis, İstanbul Medipol Üniversitesi Sağlık Bilimleri Enstitüsü).
8. Chang, C. C., Yang, M. H., Wen, H. M., & Chern, J. C., (2002). Estimation of total flavonoid content in propolis by two complementary colorimetric methods. Journal of food and drug analysis, 10(3), 178-182.

9. Foo, L., Lu, Y., Molan, A., L., Woodfield, D., R., McNabb, W., C., (2000). The phenols and prodelphinidins of white clover flowers, Phytochemistry, 54, 539-548.
10. Hao, D. C., & Xiao, P. G., (2018). Deep in shadows: Epigenetic and epigenomic regulations of medicinal plants. Chinese Herbal Medicines, 10(3), 239-248.
11. Hashemzaei, M., Delarami Far, A., Yari, A., Heravi, R. E., Tabrizian, K., Taghdisi, S. M., ... & Rezaee, R. (2017). Anticancer and apoptosis inducing effects of quercetin in vitro and in vivo. Oncology reports, 38(2), 819-828.
12. Karakoca, K., Asan-Ozusaglam, M., Cakmak, Y. S., & Teksen, M., (2015). Phenolic compounds, biological and antioxidant activities of Onobrychis armena Boiss. & Huet flower and root extracts. Chiang Mai University Journal of Natural of Sciences, 42(2), 376-392.
13. Karamian, R., & Asadbegy, M., (2016). Antioxidant activity, total phenolic and flavonoid contents of three Onobrychis species from Iran. Pharmaceutical sciences, 22(2), 112-119.
14. Marais, J. P., Mueller-Harvey, I., Brandt, E. V., & Ferreira, D., (2000). Polyphenols, condensed tannins, and other natural products in Onobrychis viciifolia (sainfoin). Journal of Agricultural and Food Chemistry, 48(8), 3440-3447.
15. Naeem, A., Hu, P., Yang, M., Zhang, J., Liu, Y., Zhu, W., & Zheng, Q. (2022). Natural Products as Anticancer Agents: Current Status and Future Perspectives. Molecules, 27(23), 8367.
16. Oyaizu M.,(1986). Studies on products of browning reactions-antioxidative activities of products of browning reaction prepared from glucosamine. The Japanese Journal of Nutrition and Dietetics 44(6), 307–315.
17. Özbek, H., Acikara, O. B., Oz, B. E., Ozbilgin, S., Kirmizi, N. I., Özrenk, B. C., ... & Saltan, G., (2019). Antidiabetic activity evaluation of Onobrychis species on alloxan-induced diabetic mice. Brazilian Journal of Pharmaceutical Sciences, 55, e18157.
18. Roumeliotis, T. I., Halabalaki, M., Alexi, X., Ankrett, D., Giannopoulou, E. G., Skaltsounis, A. L., ... & Garbis, S. D. (2013). Pharmacoproteomic study of the natural product Ebenfuran III in DU-145 prostate cancer cells: The quantitative and temporal interrogation of chemically induced cell death at the protein level. Journal of proteome research, 12(4), 1591-1603.
19. Slinkard, K., & Singleton, V. L., (1977). Total phenol analysis: automation and comparison with manual methods. American journal of enology and viticulture, 28(1), 49-55.
20. Usta, C., Yildirim, A. B., & Turker, A. U., (2014). Antibacterial and antitumour activities of some plants grown in Turkey. Biotechnology & Biotechnological Equipment, 28(2), 306-315.
21. Yu, W., Liu, C., Li, X., Yang, F., Cheng, L., Liu, C., & Song, Y. (2017). Inositol hexaphosphate suppresses colorectal cancer cell proliferation via the Akt/GSK-3β/β-catenin signaling cascade in a 1, 2-dimethylhydrazine-induced rat model. European journal of pharmacology, 805, 67-74.
22. Zhu, B., Shang, B., Li, Y., & Zhen, Y. (2016). Inhibition of histone deacetylases by trans-cinnamic acid and its antitumor effect against colon cancer xenografts in athymic mice. Molecular medicine reports, 13(5), 4159-4166