Molecular Characterisation of Some Corn (Zea mays L.) Genotypes Growing in Iğdır Province

Yıl 2024, , 1731 - 1741, 01.12.2024

Gizem Çevik Barış Eren

https://doi.org/10.21597/jist.1544279

Öz

Inter-primer binding site markers based on retrotransposons or iPBS retrotransposon markers have been useful in determining genetic diversity in a large number of organisms. The CTAB technique was employed to isolate DNA, and iPBS molecular markers were employed to conduct genetic diversity studies. Based on the analyses' findings, the genotypes exhibited a significant degree of genetic diversity, with a 100% polymorphic locus percentage. A total of 154 polymorphic bands were generated as a consequence of molecular identification experiments conducted with 12 IPBS markers. The typical polymorphism rate was determined to be 100%. Additionally, the average polymorphism (PIC) value, which quantifies the gene diversity of all markers examined in the study, was 0.228, and the average H value was 0.274. The genotypes 1 and 14 exhibited the lowest similarity ratio, with a coefficient value of 0.1600, when the DICE similarity coefficients were compared. These results were derived by comparing the DICE similarity coefficients of the samples. The analysis determined that the greatest similarity value between genotypes 4 and 3 was 0.6747. Maize genotypes are classified into four distinct subpopulations. Genotypes can be developed for reproductive research in order to demonstrate the molecular diversity of a population. It was determined that IPBS molecular markers are appropriate genetic instruments for genetic and phylogenetic analyses in maize varieties. This conclusion was attained as a consequence of the aforementioned. The data that is gathered will serve as a scientific foundation and a valuable contribution to the field of maize genetics in the future.

Anahtar Kelimeler

Molecular marker, Maize, Zea mays L.

Destekleyen Kurum

Iğdır Üniversitesi BİLİMSEL ARAŞTIRMA PROJELERİ KORDİNATÖRLÜĞÜ

Proje Numarası

ZİF0324Y07

Teşekkür

Bu araştırma Iğdır Üniversitesi Bilimsel Araştırma Projeleri Koordinatörlüğünün desteği ile (ZİF0324Y07) gerçekleştirilmiştir. Desteklerinden dolayı teşekkür ederiz.

Iğdır İlinde Yetiştiriciliği Yapılan Bazı Mısır (Zea mays L.) Genotiplerinin Moleküler Karakterizasyonu

Öz

Retrotranspozonlara dayalı primerler arası bağlanma bölgesi veya iPBS retrotranspozon belirteçleri, çok sayıda organizmada genetik çeşitliliğin belirlenmesinde yararlı olmuştur. DNA'yı izole etmek için CTAB tekniği kullanılmış ve genetik çeşitlilik çalışmaları yapmak için iPBS moleküler belirteçleri kullanılmıştır. Analizlerin bulgularına göre, genotipler %100 polimorfik lokus yüzdesi ile önemli derecede genetik çeşitlilik sergilemiştir. 12 IPBS markörü ile yürütülen moleküler tanımlama deneyleri sonucunda toplam 154 polimorfik bant üretilmiştir. Tipik polimorfizm oranı %100 olarak belirlenmiştir. Ayrıca, çalışmada incelenen tüm markörlerin gen çeşitliliğini ölçen ortalama polimorfizm (PIC) değeri 0,228 ve ortalama H değeri 0,274 olarak belirlenmiştir. DICE benzerlik katsayıları karşılaştırıldığında, 1 ve 14 numaralı genotipler 0,1600 katsayı değeri ile en düşük benzerlik oranını sergilemiştir. Bu sonuçlar, örneklerin DICE benzerlik katsayılarının karşılaştırılmasıyla elde edilmiştir. Analiz, 4 ve 3 numaralı genotipler arasındaki en büyük benzerlik değerinin 0.6747 olduğunu belirledi. Mısır genotipleri dört farklı alt popülasyon olarak sınıflandırılmıştır. Genotipler, bir popülasyonun moleküler çeşitliliğini göstermek amacıyla üreme araştırmaları için geliştirilebilir. IPBS moleküler markörlerinin mısır çeşitlerinde genetik ve filogenetik analizler için uygun genetik araçlar olduğu belirlenmiştir. Bu sonuca yukarıda bahsedilenlerin bir sonucu olarak ulaşılmıştır. Elde edilen veriler, gelecekte mısır genetiği alanına bilimsel bir temel ve değerli bir katkı olarak hizmet edecektir.

Anahtar Kelimeler

Moleküler markör, Mısır, Zea mays L.

Proje Numarası

ZİF0324Y07

Kaynakça

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