In Vitro Antioxidant Property of Convolvulus aucheri and Its NO Inhibitory Potential in LPS-activated NSCLC Cells

Öz

Latterly, scientists have turned into progressively curious about therapeutic herbs. A lot of Convolvulus taxa display different biologic activities. In this study, we aimed to evaluate nitric oxide (NO) inhibitive effect of Convolvulus aucheri in LPS-induced non-small cell lung cancer (NSCLC) cells, such as HCC78 and H1975 and determine its antioxidant capacity. While Griess assay was applied to determine the nitrite level as an index of NO production, β-carotene/linoleic acid test was used for the determining antioxidant effect. Cytotoxic activity of C. aucheri extract was detected by using CellTiter-Glo assay. The extract induced a bigger cytotoxic activity on H1975 cells than the HCC78 cells and decreased NO production in concentration-dependent manner. The highest inhibitory potential against NO formation was observed to be 113.06 μM nitrite at a concentration of 40 μg/mL in HCC78 cells. As for the antioxidant activity, C. aucheri showed the highest antioxidant activity of 70.89%. These data bring to mind that C. aucheri may be a useful source for exploration new anticancer compounds.

Anahtar Kelimeler

• lung cancer,NO assay,Convolvulaceae,C. aucheri extract,lipopolysaccharide,NO assay

Convolvulus aucheri’nin In vitro Antioksidan Özelliği ve LPS-aktif NSCLC Hücrelerinde NO Engelleyici Potansiyeli

Öz

Son zamanlarda, bilim adamları tedavi edici bitkiler hakkında giderek daha fazla ilgili hale geldiler. Birçok Convolvulus taksonu farklı biyolojik aktiviteler göstermektedir. Bu çalışmada, Convolvulus aucheri'nin LPS ile uyarılmış HCC78 ve H1975 gibi küçük hücre dışı akciğer kanseri (KHDAK) hücrelerinde nitrik oksidi (NO) inhibe edici etkisini değerlendirmeyi ve antioksidan kapasitesini belirlemeyi amaçladık. NO üretiminin bir göstergesi olan nitrit düzeyini belirlemek için Griess testi uygulanırken, antioksidan etkinin belirlenmesi için ise β-karoten/linoleik asit testi kullanıldı. C. aucheri ekstraktının sitotoksik aktivitesi, CellTiter-Glo deneyi kullanılarak tespit edildi. Ekstrakt H1975 hücrelerinde HCC78 hücrelerinden daha büyük bir sitotoksik aktiviteye sebep olmuştur ve NO üretimini konsantrasyona bağlı olarak azaltmıştır. NO oluşumuna karşı en yüksek inhibitör potansiyelin, HCC78 hücrelerinde 40 μg/mL konsantrasyonda 113.06 μM nitrit olduğu gözlenmiştir. Antioksidan aktiviteye gelince, C. aucheri en yüksek antioksidan aktivitesini %70.89 ile göstermiştir. Bu veriler, C. aucheri'nin yeni antikanser bileşiklerinin araştırılması için yararlı bir kaynak olabileceğini akla getirmektedir.

Anahtar Kelimeler

• akciğer kanseri,NO testi,Convolvulaceae,C. aucheri ekstraktı,lipopolisakkarit,NO testi

Kaynakça

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