Non-typhoidal Salmonella isolates from livestock and food samples, Kolkata, India

Yıl 2016, Cilt: 6 Sayı: 3, 113 - 120, 01.09.2016

Sudhanthiramani Sudhanthirakodi

https://doi.org/10.5799/jmid.vi.328974

Cited By: 6

Öz

Objective: Non-typhoidal Salmonella (NTS) is a major foodborne pathogen in both developed and developing countries.
The study was undertaken to determine the prevalence, serotype distribution and antimicrobial resistance profiles
of NTS isolates from livestock and food samples collected in and around Kolkata during 2012-2013.
Materials and methods: A total of 500 samples (animal, poultry and raw food) from 12 different places randomly collected
in and around Kolkata were processed as per standard procedures and analyzed for NTS. Serotyping of the isolates
was done followed by determination of antimicrobial resistance profiles and Minimum inhibitory concentrations
following disc diffusion and E-test methods.
Results: A total of 53 (10.6%) NTS isolates were recovered, with highest isolation from chicken (40%), followed by beef
and poultry cloacal samples (22% each). Total 11 Salmonella serovars were identified with predominance of Salmonella
enterica serovar Typhimurium (S. Typhimurium) (30.2%) followed by S. Kentucky (22.6%), S. Idikan (15.1%) and other
serovars. This is the first report of isolation of S. Idikan from India. Thirty-two (60.4%) isolates were pan susceptible to
all drugs tested. Percentage distribution of resistant NTS against antimicrobials were: nalidixic acid (24.5%), ampicillin
(17%), amoxicillin (17%), streptomycin (15.1%), tetracycline (11.3%), chloramphenicol (9.4%), co-trimoxazole (9.4%) and
ciprofloxacin (5.7%). Only one isolate (S. Senftenberg) was resistant to both fluroquinolones and third generation cephalosporins.
Multidrug resistance (≥3 classes of antimicrobials) was observed in 13.2% NTS isolates.
Conclusion: High prevalence of NTS in poultry and meat samples in Kolkata poses a potential risk of foodborne disease
in humans. J Microbiol Infect Dis 2016;6(3): 113-120

Anahtar Kelimeler

Non-typhoidal Salmonella, antimicrobial resistance, animals, poultry, food samples

Kaynakça

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