Adaptation of IncX3 Plasmid Encoding blaNDM-4 within A Broad Host Range

Yıl 2021, Cilt: 11 Sayı: 02, 74 - 80, 15.06.2021

Nargis Alom Choudhury Deepjyoti Paul Bhaskar Jyoti Das Debadatta Dhar Chanda Amitabha Bhattacharjee

https://doi.org/10.5799/jmid.951502

Öz

Objectives: The current study was aimed to investigate the adaptability and stability of blaNDM-4 within a broad host range and transcriptional response.
Methods: Six isolates of Escherichia coli, harboring blaNDM-4 were confirmed by PCR sequencing of the whole gene. Transformation and conjugation assay were carried out and plasmid incompatibility was determined by PCR assay. The serial passage was done for consecutive 70 days without any antibiotic pressure for both parent strain and transformants. Transcriptional expression of blaNDM-4 within a broad host range against concentration gradient imipenem stress was studied.
Results: IncX3 plasmid encoding blaNDM-4 was successfully transferred in six different hosts when imipenem (0.5 µg/ml) screen agar was used for the selection of transformants. It was also found to harbor resistance for aminoglycosides and quinolone. When checked for stability, it was observed that the plasmid was successfully expanded within all six recipients for 55th serial passages. Transcriptional expression with IncX3 was random but at a consistent level for wild type and without concentration gradient stress of imipenem. Transcriptional expression with NDM gene was variable for parent isolates though for new hosts it was showing randomly increased patterns in Proteus, E. coli, and DH5α.
Conclusion: The present study could highlight that external carbapenem pressure helps in the maintenance and expression of blaNDM-4 within different host range. This study is of epidemiological significance and will help in tracking the genetic vehicle responsible for their transmission by restricting their spread. J Microbiol Infect Dis 2021; 11(2):74-80.

Anahtar Kelimeler

Broad host range, NDM, Transcriptional expression

Kaynakça

• 1. Jones CS, Osborne DJ, Stanley J. Molecular comparison of the IncX plasmids allows division into IncX1 and IncX2 subgroups. J Gen Microbiol 1993; 139: 735–741.
• 2. Johnson TJ, Bielak EM, Fortini D, et al. Expansion of the IncX plasmid family for improved identification and typing of novel plasmids in drug-resistant Enterobacteriaceae. Plasmid 2012; 68: 43–50.
• 3. Khan AU, Nordmann P. NDM-1-producing Enterobacter cloacae and Klebsiella pneumoniae from diabetic foot ulcers in India. J Med Microbiol 2012; 61(3):454-6.
• 4. Paul D, Bhattacharjee A, Ingti B, et al. Occurrence of blaNDM-7 within IncX3-type plasmid of Escherichia coli from India. Journal of Infection and Chemotherapy. 2017; 23(4):206-210.
• 5. Choudhury NA, Paul D, Chakravarty A, Bhattacharjee A, Chanda DD. IncX3 plasmid mediated occurrence of blaNDM-4 within Escherichia coli ST448 from India. J Infect Public Health 2018; 11(1):111-114.
• 6. Paul D, Chanda DD, Maurya AP, et al. Co-carriage of blaKPC-2and blaNDM-1in clinical isolates of Pseudomonas aeruginosa associated with hospital infections from India. PLOS One 2015; 10(12):e0145823.
• 7. Paul D, Dhar D, Maurya AP, Mishra S, et al. Occurrence of co-existing blaVIM-2 and blaNDM-1 in clinical isolates of Pseudomonas aeruginosa from India. Ann Clin Microbiol Antimicrob 2016; 15:31.
• 8. Locke JB, Rahawi S, LaMarre J, Mankin AS, Shaw KJ. Genetic environment and stability of cfr in methicillin-resistant Staphylococcus aureus CM05. Antimicrob Agent Chemother 2011; AAC-05420.
• 9. Swick MC, Morgan-Linnell SK, Carlson KM, Zechiedrich L. Expression of multidrug efflux pump genes acrAB-tolC, mdfA and norE in Escherichia coli clinical isolates as a function of fluoroquinolone and multidrug resistance. Antimicrob Agents Chemother 2011; 55:921-924.
• 10. Clinical and Laboratory Standard Institute. Performance Standards for Antimicrobial Susceptibility Testing; Twenty-First Informational Supplement. 2011; M100-S21, Wayne, USA.
• 11. Chen L, Chavda KD, Fraimow HS et al. Complete nucleotide sequences of blaKPC-4- and blaKPC-5-harboring IncN and IncX plasmids from Klebsiella pneumoniae strains isolated in New Jersey. Antimicrob Agents Chemother 2013; 57: 269-276.
• 12. Du XX, Wang JF, Fu Y, et al. Genetic characteristics of blaNDM-1-positive plasmid in Citrobacter freundii isolate separated from a clinical infectious patient. J Med Microbiol 2013; 62: 1332-1337.
• 13. Albrechtova K, Dolejska M, Cizek A, et al. Dogs of nomadic pastoralists in northern Kenya are reservoirs of plasmid-mediated cephalosporinand quinolone-resistant Escherichia coli, including pandemic clone B2-O25-ST131. Antimicrob Agents Chemother 2012; 56: 4013–7.
• 14. Sonnevend A, Al Baloushi A, Ghazawi A, et al. Emergence and spread of NDM-1 producer Enterobacteriaceae with contribution of IncX3 plasmids in the United Arab Emirates. J Med Microbiol 2013; 62(Pt 7):1044-1050.
• 15. Dobiasova H, Dolejska M. Prevalence and diversity of IncX plasmids carrying fluoroquinolone and β-lactam resistance genes in Escherichia coli originating from diverse sources and geographical areas. J Antimicrob Chemother 2016; 71(8):2118–2124.
• 16. Das N, Valjavec-Gratian M, Basuray AN, et al. Multiple homeostatic mechanisms in the control of P1 plasmid replication. Proceedings of the National Academy of Sciences. 2005; 102(8):2856-2861.