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Evaluating The Effectiveness of A Non-Locally Developed Commercial Phage Cocktail on Kenyan Pseudomonas aeruginosa Isolates

Yıl 2023, Cilt: 13 Sayı: 01, 25 - 30, 15.03.2023
https://doi.org/10.5799/jmid.1265378

Öz

Objectives: The use of bacteriophages (phages) as an alternative treatment for multidrug-resistant bacteria has recently gained popularity. Phage cocktails have been proposed for broad-spectrum therapeutic effects against such resistant bacteria. However, the effectiveness of non-locally formulated phage cocktails for therapy on Kenyan isolates has yet to be evaluated and is a subject of investigation. This study aimed to determine the in vitro effectiveness of a non-locally made commercial pyophage cocktail on clinical Pseudomonas aeruginosa isolates from Kenya.
Methodology: Forty-nine P. aeruginosa isolates from Kenya were subjected to a pyophage cocktail for efficacy studies using direct spot test (DST) and efficiency of plating (EOP).
Results: The success rate of the cocktail was observed on 16.3% (8/49) isolates only and ineffective on 83.7% (41/49) isolates. Six of the eight isolates that showed cross-reactivity from DST had complete lysis with a faintly hazy background. Five of these six isolates resulted in successful and high phage progeny production in plaquing efficiency (EOP ≥ 0.5).
Conclusion: Non-locally made commercial pyophage cocktail was ineffective against the 83.7% endemic clinical strains of the Kenyan P. aeruginosa isolates, demonstrating the importance of locally derived phage cocktails against endemic and multidrug-resistant isolates. J Microbiol Infect Dis 2023; 12(4):25-30.

Yıl 2023, Cilt: 13 Sayı: 01, 25 - 30, 15.03.2023
https://doi.org/10.5799/jmid.1265378

Öz

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Ayrıntılar

Birincil Dil İngilizce
Konular Sağlık Kurumları Yönetimi
Bölüm Research Article
Yazarlar

Ivy Jepkurui Mutaı Bu kişi benim

Martin Georges Bu kişi benim

Atunga Nyachıeo Bu kişi benim

Eric Odoyo Bu kişi benim

Anthony Kebira Nyamache Bu kişi benim

Jillani Ngalla Bu kişi benim

Yayımlanma Tarihi 15 Mart 2023
Yayımlandığı Sayı Yıl 2023 Cilt: 13 Sayı: 01

Kaynak Göster

APA Mutaı, I. J., Georges, M., Nyachıeo, A., Odoyo, E., vd. (2023). Evaluating The Effectiveness of A Non-Locally Developed Commercial Phage Cocktail on Kenyan Pseudomonas aeruginosa Isolates. Journal of Microbiology and Infectious Diseases, 13(01), 25-30. https://doi.org/10.5799/jmid.1265378
AMA Mutaı IJ, Georges M, Nyachıeo A, Odoyo E, Nyamache AK, Ngalla J. Evaluating The Effectiveness of A Non-Locally Developed Commercial Phage Cocktail on Kenyan Pseudomonas aeruginosa Isolates. J Microbil Infect Dis. Mart 2023;13(01):25-30. doi:10.5799/jmid.1265378
Chicago Mutaı, Ivy Jepkurui, Martin Georges, Atunga Nyachıeo, Eric Odoyo, Anthony Kebira Nyamache, ve Jillani Ngalla. “Evaluating The Effectiveness of A Non-Locally Developed Commercial Phage Cocktail on Kenyan Pseudomonas Aeruginosa Isolates”. Journal of Microbiology and Infectious Diseases 13, sy. 01 (Mart 2023): 25-30. https://doi.org/10.5799/jmid.1265378.
EndNote Mutaı IJ, Georges M, Nyachıeo A, Odoyo E, Nyamache AK, Ngalla J (01 Mart 2023) Evaluating The Effectiveness of A Non-Locally Developed Commercial Phage Cocktail on Kenyan Pseudomonas aeruginosa Isolates. Journal of Microbiology and Infectious Diseases 13 01 25–30.
IEEE I. J. Mutaı, M. Georges, A. Nyachıeo, E. Odoyo, A. K. Nyamache, ve J. Ngalla, “Evaluating The Effectiveness of A Non-Locally Developed Commercial Phage Cocktail on Kenyan Pseudomonas aeruginosa Isolates”, J Microbil Infect Dis, c. 13, sy. 01, ss. 25–30, 2023, doi: 10.5799/jmid.1265378.
ISNAD Mutaı, Ivy Jepkurui vd. “Evaluating The Effectiveness of A Non-Locally Developed Commercial Phage Cocktail on Kenyan Pseudomonas Aeruginosa Isolates”. Journal of Microbiology and Infectious Diseases 13/01 (Mart 2023), 25-30. https://doi.org/10.5799/jmid.1265378.
JAMA Mutaı IJ, Georges M, Nyachıeo A, Odoyo E, Nyamache AK, Ngalla J. Evaluating The Effectiveness of A Non-Locally Developed Commercial Phage Cocktail on Kenyan Pseudomonas aeruginosa Isolates. J Microbil Infect Dis. 2023;13:25–30.
MLA Mutaı, Ivy Jepkurui vd. “Evaluating The Effectiveness of A Non-Locally Developed Commercial Phage Cocktail on Kenyan Pseudomonas Aeruginosa Isolates”. Journal of Microbiology and Infectious Diseases, c. 13, sy. 01, 2023, ss. 25-30, doi:10.5799/jmid.1265378.
Vancouver Mutaı IJ, Georges M, Nyachıeo A, Odoyo E, Nyamache AK, Ngalla J. Evaluating The Effectiveness of A Non-Locally Developed Commercial Phage Cocktail on Kenyan Pseudomonas aeruginosa Isolates. J Microbil Infect Dis. 2023;13(01):25-30.