The Effect of BclI and N363S Glucocorticoid Receptor Gene (NR3C1) Polymorphisms in Turkish Patients with Type 2 Diabetes Mellitus
Abstract
This study aims to examine the relationship between the prevalence of glucocorticoid receptor (GR) gene polymorphisms and type 2 diabetes mellitus (T2DM) in Turkish individuals. Clinical and laboratory findings of patients diagnosed with T2DM and healthy controls were recorded, and blood samples were collected for genetic analysis. Following DNA isolation, genotyping was performed for the BclI and N363S polymorphisms of the Nuclear Subfamily 3 Group C Member 1 (NR3C1) gene using the Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) method. The genotype frequencies of the BclI (rs41423247) polymorphism showed a significant association with T2DM; the CC (34.1%) and CG (56.5%) genotypes were more frequent in the patient group compared to the control group, with a calculated risk ratio for the C allele of 4.762. No significant association was observed between the N363S (rs6195) polymorphism and T2DM (p>0.05). Overall, findings indicate that BclI polymorphism contributes to T2DM susceptibility in this population, whereas the N363S variant does not appear to influence disease risk.
Keywords
Glucocorticoid receptor gene, polymorphism, type 2 diabetes
Supporting Institution
This work was supported by the Scientific Research Projects Coordination Unit at Gazi University, Grant number: 05/2013-12.
Project Number
05/2013-12
Ethical Statement
The research was reviewed and approved by the Clinical Research Ethics Committee of Gazi University, Approval number: 190. This paper is derived from the first author’s master’s thesis supervised by the second author.
Thanks
This work was supported by the Scientific Research Projects Coordination Unit at Gazi University, Grant number: 05/2013-12.
References
- A. Kamba, M. Daimon, H. Murakami, H. Otaka, K. Matsuki, E. Sato, J. Tanabe, S.Takayasu, Y. Matsuhashi, M. Yanagimachi, K. Terui, K. Kageyama, I. Tokuda, I. Takahashi, S. Nakaji, Association between Higher Serum Cortisol Levels and Decreased Insulin Secretion in a General Population, PLoS One 11 (11) (2016) e0166077.
- N. B. Kasim, H. Z. Huri, S. R. Vethakkan, L. Ibrahim, B. M. Abdullah, Genetic polymorphisms associated with overweight and obesity in uncontrolled Type 2 diabetes mellitus, Biomarkers in Medicine 10 (4) (2016) 403–415.
- S. K. Kota, L. K. Meher, S. Jammula, S. K. Kota, K. D. Modi, Genetics of type 2 diabetes mellitus and other specific types of diabetes; its role in treatment modalities. Diabetes and Metabolic Syndrome 6 (1) (2012) 54–58.
- D. J. Magliano, E. J. Boyko, Diabetes Atlas (2025), https://diabetesatlas.org/resources/idf-diabetes-atlas-2025/, Accessed 10 June 2025.
- X. Han, Y. Wei, H. Hu, J. Wang, Z. Li, F. Wang, T. Long, J. Yuan, P. Yao, S. Wei, Y. Wang, X. Zhang, H. Guo, H. Yang, T. Wu, M. He, Genetic risk, a healthy lifestyle, and type 2 diabetes: the Dongfeng-Tongji cohort study, The Journal of Clinical Endocrinology and Metabolism 105 (4) (2020) 1242-1250.
- K. Matsuda, PCR-Based Detection Methods for Single-Nucleotide Polymorphism or Mutation: Real-Time PCR and Its Substantial Contribution Toward Technological Refinement, Advances in Clinical Chemistry 80 (2017) 45–72.
- M. A. Orlovsky, Allelic polymorphism of glucocorticoid receptor NR3C1 (GR): from molecular biology to clinical implications, Biopolymers and Cell 28 (5) (2012) 338–351.
- J. Etemadi, S. Abediazar, T. Majidi, S. Z. Vahed, R. Motavalli, B. Niknafs, M. J. Nakhjavani, Analysis of glucocorticoid receptor gene polymorphisms in kidney recipients with post-transplant diabetes, Journal of Renal Injury Prevention (2022).
- L. Feldkamp, L. Müller, T. Deutschbein, M. Detomas, S. Hahner, C. J. Strasburger, H. Künzel, A. Oßwald, L. Braun, G. Rubinstein, M. Reincke, M. Quinkler, T. Kienitz, Glucocorticoid receptor polymorphism BclI with increased glucocorticoid sensitivity has a positive influence on Quality of Life in Endogenous Cushing's syndrome in remission, European Journal of Endocrinology 188 (4) (2023) 366–374.
- K. M. Salah, M. M. Al Shafie, O. A. Gaber, M. T. Awad, Association between Glucocorticosteroid Receptor (NR3C1) Gene Polymorphism and Bronchial Asthma in Children, Zagazig University Medical Journal 26 (1) (2020) 123–132.