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Yıl 2019, Cilt 2, Sayı 1, 73 - 76, 31.07.2019

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Kaynakça

  • 1. Adams M.D., Celniker S.E., Holt R.A., Evans C.A. & Gocayne J.D. (2000). The Genome Sequence of Drosophila melanogaster. Science, New Series, Vol. 287, No.5461:2185-2195.
  • 2. Augustyniak M. Gladysz M. Dziewie M. 2015. The Comet assay in insects—Status, prospects and benefits for science. Mutat. Res.: Rev. Mutat. Res. http://dx.doi.org/10.1016/j.mrrev. 2015.09.001.
  • 3. Carmona E.R., Guecheva T.N., Creus A. & Marco R. (2011). Proposal of an In Vivo Comet Assay Using Haemocytes of Drosophila melanogaster. Environmental and Molecular Mutagenesis. 52:165-169.
  • 4. Collins A.R. (2004). The Comet Assay for DNA Damage and Repair; Principles, Applications, and Limitations. Molecular Biotechnology. 26: 249-261.
  • 5. Dhawan A., Bajpayee M., Pandey A.K. & Parmar D. (2009). Protocol for the single cell gel electrophoresis/comet assay for rapid genotoxicity assessment. Developmental Toxicology Division Industrial Toxicology Research Centre Marg, Lucknow 226001U.P. India, Retrieved September 21, 2013 from http// www.cometassayindia.org / Protocol %20for %20 Comet %20Assay.PDF.
  • 6. Fairbairn D.W., Olive P.L. & O'Neill K.L. (1995). The comet assay: a comprehensive review. Mutation Research. 339:37-59.
  • 7. Gaivão I. & Sierra L.M. (2014). Drosophila comet assay: insights, uses, and future perspectives. Frontiers in Genetics. August 2014 | Volume5 | Article 304:1-8.
  • 8. Gupta S.C., Mishra M., Sharma A., Balaji T.G.R.D., Kumar R., Mishra RK. & Chowdhuri D.K. (2010). Chlorpyrifos induces apoptosis and DNA damage in Drosophila through generation of reactive oxygen species. Ecotoxicology and Environmental Safety. 73:1415–1423.
  • 9. Langie S.A.S., Azqueta A. & Collins A.R. (2015). The comet assay: past, present, and future. Frontiers in Genetics|www.frontiersin.org 1 August 2015|Volume6|Article266:1-3.
  • 10. Olive P.L. & Banáth J.P. (2006). The comet assay: a method to measure DNA damage in individual cells. Nature Protocols | VOL.1 NO.1: 23-29.
  • 11. Ong C., Yung L.Y.L., Cai Y., Bay B.H. & Baeg G.H. (2014). Drosophila melanogaster as a model organism to study nanotoxicity. Nanotoxicology. ISSN: 1743-5390 (print), 1743-5404 (electronic) Nanotoxicology, Early Online: 1–8. DOI: 10.3109/17435390.2014.940405.
  • 12. Piper M.D.W. & Partridge L. (2018). Drosophila as a model for ageing. BBA - Molecular Basis of Disease. 1864:2707–2717.
  • 13. Sezginer H. &Dane F. (2016). Genotoxic Analysis Methods of Toxic Substances. Turkish Journal of Scientific Compilations. 9 (1): 50-55.
  • 14. Sayed A.E.D.H., Watanabe-Asaka T., Oda S., Kashiwada S.& Mitani H. (2018). γ-H2AX foci as indication for the DNA damage in erythrocytes of medaka (Oryzias latipes) intoxicated with 4-nonylphenol. Environmental Science and Pollution Research. https://doi.org/10.1007/s11356-018-2985-z.
  • 15. Tice R.R., Agurell E., Anderson D., Burlinson B., Hartmann A., Kobayashi H., Miyamae Y., Rojas E., Ryu J-C. & Sasaki Y.F. (2000). Single Cell Gel/Comet Assay: Guidelines for in vitro and in vivo Genetic Toxicology Testing. Environmental and Molecular Mutagenesis. 35:206-221.
  • 16. Tiwari A.K., Pragya P., Ram K.R. & Chowdhuri D.K. (2011). Environmental chemical mediated male reproductive toxicity: Drosophila melanogaster as an alternate animal model. Theriogenology 76:197–216.

AN OPTIMIZED COMET ASSAY PROTOCOL FOR DROSOPHILA MELANOGASTER

Yıl 2019, Cilt 2, Sayı 1, 73 - 76, 31.07.2019

Öz

Genotoxins can cause various disorders in chromosome and DNA structure such as gene mutations, chromosome abnormalities and DNA chain breaks.  This situation has been associated with the concept of genetic toxicity.  Genotoxicity tests have been developed to predict the possible effects of genotoxins on all living things in nature.  The single cell gel electrophoresis assay (SCGE, also known as comet assay) is used as the fastest, easy and sensitive. The Comet method is based on distinctive migration characteristics of DNA molecules having different electrical charges and molecular weights at alkaline pH. According to this method, nucleolus’ and cells can be visualized by staining with a fluorescent dye by first placing between the agarose layers and then running at alkaline electrophoresis buffer and lysis solution and lastly applying neutralization procedure. The displayed DNA fractures are measured and evaluated according to tail length. DNA images are graded according to the degree of damage. DNAs that are not damaged are evaluated as arbitrary units (AU) by scoring 0 and damage DNAs are scored from 1 to 4 according to the degree of damage. Comet assay is a useful method in evaluating the potential toxic effect.  Drosophila melanogaster is the most important non-mammalian organism used in the fields of medicine and biology. Drosophila melanogaster has been used for many years as an in vivo model organism for potential toxicity and genotoxicity studies. It is important to determine the damage of genotoxins on DNA. It was preferred because it was easy to obtain and grow in the laboratory, the larvae were cheap and the life cycle was short-lived. Comet analysis using Drosophila melanogaster tissues to determine the damage of various genotoxins on DNA is an effective and easy method. This study aims to explain the alkaline Comet assay aimed to determine the effect of general various genotoxins on Drosophila melanogaster tissues. 

Kaynakça

  • 1. Adams M.D., Celniker S.E., Holt R.A., Evans C.A. & Gocayne J.D. (2000). The Genome Sequence of Drosophila melanogaster. Science, New Series, Vol. 287, No.5461:2185-2195.
  • 2. Augustyniak M. Gladysz M. Dziewie M. 2015. The Comet assay in insects—Status, prospects and benefits for science. Mutat. Res.: Rev. Mutat. Res. http://dx.doi.org/10.1016/j.mrrev. 2015.09.001.
  • 3. Carmona E.R., Guecheva T.N., Creus A. & Marco R. (2011). Proposal of an In Vivo Comet Assay Using Haemocytes of Drosophila melanogaster. Environmental and Molecular Mutagenesis. 52:165-169.
  • 4. Collins A.R. (2004). The Comet Assay for DNA Damage and Repair; Principles, Applications, and Limitations. Molecular Biotechnology. 26: 249-261.
  • 5. Dhawan A., Bajpayee M., Pandey A.K. & Parmar D. (2009). Protocol for the single cell gel electrophoresis/comet assay for rapid genotoxicity assessment. Developmental Toxicology Division Industrial Toxicology Research Centre Marg, Lucknow 226001U.P. India, Retrieved September 21, 2013 from http// www.cometassayindia.org / Protocol %20for %20 Comet %20Assay.PDF.
  • 6. Fairbairn D.W., Olive P.L. & O'Neill K.L. (1995). The comet assay: a comprehensive review. Mutation Research. 339:37-59.
  • 7. Gaivão I. & Sierra L.M. (2014). Drosophila comet assay: insights, uses, and future perspectives. Frontiers in Genetics. August 2014 | Volume5 | Article 304:1-8.
  • 8. Gupta S.C., Mishra M., Sharma A., Balaji T.G.R.D., Kumar R., Mishra RK. & Chowdhuri D.K. (2010). Chlorpyrifos induces apoptosis and DNA damage in Drosophila through generation of reactive oxygen species. Ecotoxicology and Environmental Safety. 73:1415–1423.
  • 9. Langie S.A.S., Azqueta A. & Collins A.R. (2015). The comet assay: past, present, and future. Frontiers in Genetics|www.frontiersin.org 1 August 2015|Volume6|Article266:1-3.
  • 10. Olive P.L. & Banáth J.P. (2006). The comet assay: a method to measure DNA damage in individual cells. Nature Protocols | VOL.1 NO.1: 23-29.
  • 11. Ong C., Yung L.Y.L., Cai Y., Bay B.H. & Baeg G.H. (2014). Drosophila melanogaster as a model organism to study nanotoxicity. Nanotoxicology. ISSN: 1743-5390 (print), 1743-5404 (electronic) Nanotoxicology, Early Online: 1–8. DOI: 10.3109/17435390.2014.940405.
  • 12. Piper M.D.W. & Partridge L. (2018). Drosophila as a model for ageing. BBA - Molecular Basis of Disease. 1864:2707–2717.
  • 13. Sezginer H. &Dane F. (2016). Genotoxic Analysis Methods of Toxic Substances. Turkish Journal of Scientific Compilations. 9 (1): 50-55.
  • 14. Sayed A.E.D.H., Watanabe-Asaka T., Oda S., Kashiwada S.& Mitani H. (2018). γ-H2AX foci as indication for the DNA damage in erythrocytes of medaka (Oryzias latipes) intoxicated with 4-nonylphenol. Environmental Science and Pollution Research. https://doi.org/10.1007/s11356-018-2985-z.
  • 15. Tice R.R., Agurell E., Anderson D., Burlinson B., Hartmann A., Kobayashi H., Miyamae Y., Rojas E., Ryu J-C. & Sasaki Y.F. (2000). Single Cell Gel/Comet Assay: Guidelines for in vitro and in vivo Genetic Toxicology Testing. Environmental and Molecular Mutagenesis. 35:206-221.
  • 16. Tiwari A.K., Pragya P., Ram K.R. & Chowdhuri D.K. (2011). Environmental chemical mediated male reproductive toxicity: Drosophila melanogaster as an alternate animal model. Theriogenology 76:197–216.

Ayrıntılar

Birincil Dil İngilizce
Konular Biyoloji
Bölüm Makaleler
Yazarlar

Fahriye ZEMHERİ NAVRUZ (Sorumlu Yazar)
Bartın Üniversitesi
0000-0003-1744-1091
Türkiye

Yayımlanma Tarihi 31 Temmuz 2019
Yayınlandığı Sayı Yıl 2019, Cilt 2, Sayı 1

Kaynak Göster

Bibtex @derleme { jonas572637, journal = {Bartın University International Journal of Natural and Applied Sciences}, issn = {}, eissn = {2667-5048}, address = {Bartın Üniversitesi Fen Bilimleri Enstitüsü Ağdacı Kampüsü 74100-BARTIN}, publisher = {Bartın Üniversitesi}, year = {2019}, volume = {2}, pages = {73 - 76}, doi = {}, title = {AN OPTIMIZED COMET ASSAY PROTOCOL FOR DROSOPHILA MELANOGASTER}, key = {cite}, author = {Zemheri Navruz, Fahriye} }
APA Zemheri Navruz, F. (2019). AN OPTIMIZED COMET ASSAY PROTOCOL FOR DROSOPHILA MELANOGASTER . Bartın University International Journal of Natural and Applied Sciences , 2 (1) , 73-76 . Retrieved from https://dergipark.org.tr/tr/pub/jonas/issue/47366/572637
MLA Zemheri Navruz, F. "AN OPTIMIZED COMET ASSAY PROTOCOL FOR DROSOPHILA MELANOGASTER" . Bartın University International Journal of Natural and Applied Sciences 2 (2019 ): 73-76 <https://dergipark.org.tr/tr/pub/jonas/issue/47366/572637>
Chicago Zemheri Navruz, F. "AN OPTIMIZED COMET ASSAY PROTOCOL FOR DROSOPHILA MELANOGASTER". Bartın University International Journal of Natural and Applied Sciences 2 (2019 ): 73-76
RIS TY - JOUR T1 - AN OPTIMIZED COMET ASSAY PROTOCOL FOR DROSOPHILA MELANOGASTER AU - Fahriye Zemheri Navruz Y1 - 2019 PY - 2019 N1 - DO - T2 - Bartın University International Journal of Natural and Applied Sciences JF - Journal JO - JOR SP - 73 EP - 76 VL - 2 IS - 1 SN - -2667-5048 M3 - UR - Y2 - 2019 ER -
EndNote %0 Bartın Üniversitesi Uluslararası Fen Bilimleri Dergisi AN OPTIMIZED COMET ASSAY PROTOCOL FOR DROSOPHILA MELANOGASTER %A Fahriye Zemheri Navruz %T AN OPTIMIZED COMET ASSAY PROTOCOL FOR DROSOPHILA MELANOGASTER %D 2019 %J Bartın University International Journal of Natural and Applied Sciences %P -2667-5048 %V 2 %N 1 %R %U
ISNAD Zemheri Navruz, Fahriye . "AN OPTIMIZED COMET ASSAY PROTOCOL FOR DROSOPHILA MELANOGASTER". Bartın University International Journal of Natural and Applied Sciences 2 / 1 (Temmuz 2019): 73-76 .
AMA Zemheri Navruz F. AN OPTIMIZED COMET ASSAY PROTOCOL FOR DROSOPHILA MELANOGASTER. JONAS. 2019; 2(1): 73-76.
Vancouver Zemheri Navruz F. AN OPTIMIZED COMET ASSAY PROTOCOL FOR DROSOPHILA MELANOGASTER. Bartın University International Journal of Natural and Applied Sciences. 2019; 2(1): 73-76.
IEEE F. Zemheri Navruz , "AN OPTIMIZED COMET ASSAY PROTOCOL FOR DROSOPHILA MELANOGASTER", Bartın University International Journal of Natural and Applied Sciences, c. 2, sayı. 1, ss. 73-76, Tem. 2019