Preparation and Determination of In Vivo and In Vitro Performance of Doxycycline Imprinted Contact Lenses for Corneal Neovascularization Treatment

Yıl 2018, Cilt: 5 Sayı: 3, 1185 - 1192, 01.09.2018

Sibel Erkal Ilhan Özge Kürkçüoğlu Tuğçe Inan Özde Zeynep Güner Dilek Dalgakıran Begüm Okutan Gamze Torun Köse Ayça Kırmızı Aylin Okçu Heper Özlem Gürses F. Seniha Güner

https://doi.org/10.18596/jotcsa.428846

Cited By: 1

Öz

The aim of this study is to develop doxycycline
imprinted contact lenses that will be used in the treatment of corneal
neovascularization, which can eventually cause blindness. For this purpose,
doxycycline imprinted contact lenses were first prepared, and then they were
loaded with doxycycline and their in
vitro and in vivo performances
were determined. In the synthesis of the contact lenses, 2-hydroxyethyl
methacrylate was used as a backbone monomer. The functional monomer was
selected as itaconic acid using molecular simulations. Doxycycline release
profile of the lenses was determined in NaCl solution and their cytotoxic
response was investigated on retinal pigment epithelium cells. In vivo experiments in rat models were
performed to study the treatment patterns. The rats were sacrificed fifteen
days after treatment, and clinical examination under optical microscope was
performed to evaluate neovascularization, infiltration of inflammatory cells,
and corneal epithelial changes. In conclusion; doxycycline imprinted contact
lenses promise as an effective treatment method for corneal neovascularization.

Anahtar Kelimeler

Corneal neovascularization, Contact lens, Doxycycline

Kaynakça

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