Antioxidant Behavior of Hyoscyamus Niger Having Narcotic Effect on Heavy Metal Reduction and Radical Scavenging

Abstract

Medicinal plants with various healing effects are essential for alternative therapy and adverse environmental conditions in daily life such as stress and heavy metals are both of the main causes of oxidative stress in cells. In this context, the regulation of free radical concentration associated with heavy metal toxicity and heavy metal elimination is essential for the health of cellular metabolic pathways against neurodegenerative diseases and cancer. Medicinal plants are important actors of alternative therapy and indispensable candidates for this effect. Hyoscyamus niger (H. niger) is a member of Solanaceae family with narcotic effect and is used in the treatment of various diseases besides as used as a painkiller. In this study, H. niger having therapeutic properties in various diseases was collected from Patnos/Ağrı province at 1635 m height. The ethanol extract was prepared using its leaves and several biological properties were evaluated to improve the moderate literature of H. niger. For this purpose, first, the heavy metal reduction tests (cupric ions reduction capacity by CUPRAC method and ferric ions reduction capacity by FRAP method) and DPPH radical scavenging activity were performed, and second, total antioxidant activity was investigated to verify the results of heavy metal reduction and radical scavenging effects.

Keywords

• Antioxidant
• BHA
• Solanaceae
• Radical
• heavy metal
• Hyoscyamus niger

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