Biophysical and thermodynamical insights into the interaction of mefenamic acid with human serum albumin, based on combined multi-spectroscopic and molecular modeling approaches

Golnaz Parvizifard; Mostafa Zakariazadeh; Hossein Haghaei; Mina Shaban; Somaieh Soltani

Biophysical and thermodynamical insights into the interaction of mefenamic acid with human serum albumin, based on combined multi-spectroscopic and molecular modeling approaches

Abstract

The molecular mechanism of interaction between Mefenamic acid (MA) and human serum albumin (HSA) was investigated. UV-Visible absorption, fluorescence, and FT-IR spectroscopies, with molecular docking, have been used for assay of complex formation, quenching mechanism study, thermodynamic evaluations, and molecular details of the interaction mechanism. The quenching constant (Ksv) of 1.51×105 M-1 was obtained, while the results are indicating the dynamic quenching mechanism. The number of binding sites (n) and apparent binding constants (KA) were 1.51 and 6.55×107 M-1, respectively that resembles positive cooperativity and and strong binding of MA to HSA. The negative sign of standard enthalpy change (ΔH = -88.51 KJ/mol), standard entropy change (ΔS = -146.24 J/mol K), and Gibbs free energy (ΔG = -44.93 KJ/mol) indicated that the van der Waals interactions and hydrogen bonds are facilitating the MA-HSA complex formation. Addition of the metal ions, glucose, urea, and basic pHs decrease the MA-HSA binding constant. Molecular docking simulation showed that mainly positively charged amino acid residues contribute to the MA-HSA interaction.

Keywords

References

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Details

Primary Language

English

Subjects

Pharmacology and Pharmaceutical Sciences (Other)

Journal Section

Research Article

Authors

Golnaz Parvizifard This is me
0000-0003-4111-3055
Iran

Mostafa Zakariazadeh This is me
0000-0003-3247-3876
Iran

Hossein Haghaei This is me
0000-0001-9453-0886
Iran

Mina Shaban This is me
0000-0003-3643-3687
Iran

Somaieh Soltani ^*
0000-0002-0102-2960
Iran

Publication Date

June 28, 2025

Submission Date

April 19, 2023

Acceptance Date

July 1, 2023

Published in Issue

Year 2024 Volume: 28 Number: 1

IZ

https://izlik.org/JA23LY97YJ

Cite

RIS / Bibtex

APA

Parvizifard, G., Zakariazadeh, M., Haghaei, H., Shaban, M., & Soltani, S. (2025). Biophysical and thermodynamical insights into the interaction of mefenamic acid with human serum albumin, based on combined multi-spectroscopic and molecular modeling approaches. Journal of Research in Pharmacy, 28(1), 372-384. https://izlik.org/JA23LY97YJ

AMA

1.Parvizifard G, Zakariazadeh M, Haghaei H, Shaban M, Soltani S. Biophysical and thermodynamical insights into the interaction of mefenamic acid with human serum albumin, based on combined multi-spectroscopic and molecular modeling approaches. J. Res. Pharm. 2025;28(1):372-384. https://izlik.org/JA23LY97YJ

Chicago

Parvizifard, Golnaz, Mostafa Zakariazadeh, Hossein Haghaei, Mina Shaban, and Somaieh Soltani. 2025. “Biophysical and Thermodynamical Insights into the Interaction of Mefenamic Acid With Human Serum Albumin, Based on Combined Multi-Spectroscopic and Molecular Modeling Approaches”. Journal of Research in Pharmacy 28 (1): 372-84. https://izlik.org/JA23LY97YJ.

EndNote

Parvizifard G, Zakariazadeh M, Haghaei H, Shaban M, Soltani S (June 1, 2025) Biophysical and thermodynamical insights into the interaction of mefenamic acid with human serum albumin, based on combined multi-spectroscopic and molecular modeling approaches. Journal of Research in Pharmacy 28 1 372–384.

IEEE

[1]G. Parvizifard, M. Zakariazadeh, H. Haghaei, M. Shaban, and S. Soltani, “Biophysical and thermodynamical insights into the interaction of mefenamic acid with human serum albumin, based on combined multi-spectroscopic and molecular modeling approaches”, J. Res. Pharm., vol. 28, no. 1, pp. 372–384, June 2025, [Online]. Available: https://izlik.org/JA23LY97YJ

ISNAD

Parvizifard, Golnaz - Zakariazadeh, Mostafa - Haghaei, Hossein - Shaban, Mina - Soltani, Somaieh. “Biophysical and Thermodynamical Insights into the Interaction of Mefenamic Acid With Human Serum Albumin, Based on Combined Multi-Spectroscopic and Molecular Modeling Approaches”. Journal of Research in Pharmacy 28/1 (June 1, 2025): 372-384. https://izlik.org/JA23LY97YJ.

JAMA

1.Parvizifard G, Zakariazadeh M, Haghaei H, Shaban M, Soltani S. Biophysical and thermodynamical insights into the interaction of mefenamic acid with human serum albumin, based on combined multi-spectroscopic and molecular modeling approaches. J. Res. Pharm. 2025;28:372–384.

MLA

Parvizifard, Golnaz, et al. “Biophysical and Thermodynamical Insights into the Interaction of Mefenamic Acid With Human Serum Albumin, Based on Combined Multi-Spectroscopic and Molecular Modeling Approaches”. Journal of Research in Pharmacy, vol. 28, no. 1, June 2025, pp. 372-84, https://izlik.org/JA23LY97YJ.

Vancouver

1.Golnaz Parvizifard, Mostafa Zakariazadeh, Hossein Haghaei, Mina Shaban, Somaieh Soltani. Biophysical and thermodynamical insights into the interaction of mefenamic acid with human serum albumin, based on combined multi-spectroscopic and molecular modeling approaches. J. Res. Pharm. [Internet]. 2025 Jun. 1;28(1):372-84. Available from: https://izlik.org/JA23LY97YJ