The extraction and determination of ellagic acid and resveratrol in blueberry species by HPLC-DAD and LC-MS/MS

Suleyman Seyhan; Guler Yalcin; Serap Ayaz Seyhan

The extraction and determination of ellagic acid and resveratrol in blueberry species by HPLC-DAD and LC-MS/MS

Abstract

Ellagic acid (EA) and resveratrol (RES) are phenolic compounds naturally found in fruits. However, a significant amount of RES and EA is present in berries and their effective isolation enables the production of functional foods. In this study, EA and RES found in four highbush (Vaccinium corymbosum) blueberry varieties (Bluecrop, Brigitta, Darrow and Bluejay) were analyzed simultaneously for the first time. A chromatographic method was developed for EA and RES by HPLC-DAD and LC- MS/MS. Furthermore, pressurized liquid extraction (PLE) method was performed for the extraction of EA and RES from blueberry samples. LOD (limit of detection) and LOQ (limit of quantification) were determined as 0.1898x10-6 mol.L-1 and 0.5694x10-6 mol.L-1 for RES, 0.1271x10-6 mol.L-1 and 0.3814x10-6 mol.L-1 for EA, respectively. All the results were reevaluated according to 91.85% and 84.97% recovery value, for EA and RES, respectively. The amount of EA and RES were found out in the range of 1.65 - 9.16 mg/kg and 2.95 - 9.31 mg/kg in the lyophilized blueberry varieties.

Keywords

References

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Details

Primary Language

English

Subjects

Pharmaceutical Analytical Chemistry

Journal Section

Research Article

Authors

Suleyman Seyhan This is me
0000-0003-4081-3324
Türkiye

Guler Yalcin This is me
0000-0002-7640-7857
Türkiye

Serap Ayaz Seyhan ^*
0000-0001-5908-2766
Türkiye

Publication Date

June 28, 2025

Submission Date

December 14, 2022

Acceptance Date

December 21, 2022

Published in Issue

Year 2023 Volume: 27 Number: 1

IZ

https://izlik.org/JA48HE74YS

Cite

RIS / Bibtex

APA

Seyhan, S., Yalcin, G., & Ayaz Seyhan, S. (2025). The extraction and determination of ellagic acid and resveratrol in blueberry species by HPLC-DAD and LC-MS/MS. Journal of Research in Pharmacy, 27(1), 311-319. https://izlik.org/JA48HE74YS

AMA

1.Seyhan S, Yalcin G, Ayaz Seyhan S. The extraction and determination of ellagic acid and resveratrol in blueberry species by HPLC-DAD and LC-MS/MS. J. Res. Pharm. 2025;27(1):311-319. https://izlik.org/JA48HE74YS

Chicago

Seyhan, Suleyman, Guler Yalcin, and Serap Ayaz Seyhan. 2025. “The Extraction and Determination of Ellagic Acid and Resveratrol in Blueberry Species by HPLC-DAD and LC-MS MS”. Journal of Research in Pharmacy 27 (1): 311-19. https://izlik.org/JA48HE74YS.

EndNote

Seyhan S, Yalcin G, Ayaz Seyhan S (June 1, 2025) The extraction and determination of ellagic acid and resveratrol in blueberry species by HPLC-DAD and LC-MS/MS. Journal of Research in Pharmacy 27 1 311–319.

IEEE

[1]S. Seyhan, G. Yalcin, and S. Ayaz Seyhan, “The extraction and determination of ellagic acid and resveratrol in blueberry species by HPLC-DAD and LC-MS/MS”, J. Res. Pharm., vol. 27, no. 1, pp. 311–319, June 2025, [Online]. Available: https://izlik.org/JA48HE74YS

ISNAD

Seyhan, Suleyman - Yalcin, Guler - Ayaz Seyhan, Serap. “The Extraction and Determination of Ellagic Acid and Resveratrol in Blueberry Species by HPLC-DAD and LC-MS MS”. Journal of Research in Pharmacy 27/1 (June 1, 2025): 311-319. https://izlik.org/JA48HE74YS.

JAMA

1.Seyhan S, Yalcin G, Ayaz Seyhan S. The extraction and determination of ellagic acid and resveratrol in blueberry species by HPLC-DAD and LC-MS/MS. J. Res. Pharm. 2025;27:311–319.

MLA

Seyhan, Suleyman, et al. “The Extraction and Determination of Ellagic Acid and Resveratrol in Blueberry Species by HPLC-DAD and LC-MS MS”. Journal of Research in Pharmacy, vol. 27, no. 1, June 2025, pp. 311-9, https://izlik.org/JA48HE74YS.

Vancouver

1.Suleyman Seyhan, Guler Yalcin, Serap Ayaz Seyhan. The extraction and determination of ellagic acid and resveratrol in blueberry species by HPLC-DAD and LC-MS/MS. J. Res. Pharm. [Internet]. 2025 Jun. 1;27(1):311-9. Available from: https://izlik.org/JA48HE74YS