Identification of major phenolic compounds of Aloe vera and its protective effect on ovaries under oxidative stress in diabetic rats

Serdal Kurt; Uğur Şeker; Murat Onur Yazlık; Engin Er; Muhammed Mesud Hürkul; Sevda Söker

Identification of major phenolic compounds of Aloe vera and its protective effect on ovaries under oxidative stress in diabetic rats

Abstract

The study investigated the major phenolic compounds of Aloe vera leaf (AVL) by the high-performance liquid chromatography (HPLC) method, and its protective effect on ovaries under oxidative stress of streptozotocin- induced diabetic rats. The study was conducted on control (untreated healthy rats; C), diabetes (untreated diabetic rats; D) and diabetes+A. vera treatment (diabetic rats treated with A. vera; D+A) groups. D+A group was given an ethanolic extract of A. vera (300 mg/kg) for 14 days. Major phenolic compounds of AVL were chlorogenic acid and rutin. alanine transaminase (ALT) and aspartate aminotransferase (AST) levels increased in D group compared to others groups (P<0.01). Alkaline phosphatase (ALP) and albumin levels were lower and higher in the C group than in other groups, respectively (P<0.01). In the D group compared to other groups, oxidative stress index (OSI) and total oxidant status levels increased, while total antioxidant status level decreased in both ovarian tissues and blood (P<0.01). Preantral and antral follicle counts decreased in the D group according to other groups (P<0.01) and atretic follicle count increased (P<0.05). Proliferating cell nuclear antigen (PCNA) expression level (P<0.01), and B-cell lymphoma-2-associated-X- protein (Bax; P<0.01) and tumor necrosis factor-alpha (TNF-α) expression levels (P<0.05) decreased and increased in group D according to other groups, respectively. In conclusion, treatment with AVL decreased OSI, improved follicular dynamics, and restored Bax, TNF-α and PCNA expression in diabetic rats.

Keywords

References

Mohamed EAK. Anti-diabetic, antihypercholestermic and antioxidativeeffect of Aloe vera gel extract in alloxaninduceddiabeticrats. Aust J Basic ApplSci. 2011;5: 1321-1327.
Shima H, Naeem E, Hassan M, Hossein N. Aloe vera gel protects ovarian structure in diabetic rat. AEJTS. 2011;3(3): 197-203.
Rosenbaum D, Haber RS, Dunaif A. Insulin resistance in polycystic ovary syndrome: decreased expression of GLUT-4 glucose transporters in adipocytes. Am J Physiol Endocrinol Metab. 1993;264: E197-E202. https://doi.org/10.1152/ajpendo.1993.264.2.E197
Nayki U, Onk D, Balci G, Nayki C, Onk A,Gunay M. The effects of diabetes mellitus on ovarian injury and reserve: an experimental study. Gynecol Obstet Invest. 2016;81: 424-429. https://doi.org/10.1159/000442287
Agarwal A, Gupta S, Sharma RK. Role of oxidative stress in female reproduction. Reproductive biology and endocrinology. 2005; 3(1): 1-21.
Wang S, He G, Chen M, Zuo T, Xu W, Liu X. The role of antioxidant enzymes in the ovaries. Oxid Med Cell Long. 2027;2017: 1-14. https://doi.org/10.1155/2017/4371714
Sen S, Chakraborty R, Sridhar C, Reddy Y, De B. Free radicals, antioxidants, diseases and phytomedicines: current status and future prospect. International Journal of Pharmaceutical Sciences and Re-search. 2010;3 (1) : 91-100
Zhong, R.Z., Zhou, & D.W. Oxidative stress and role of natural plant derived antioxidants in animal reproduction. J Integr Agric. 2013;12: 1826-1838. https://doi.org/10.1016/S2095-3119(13)60412-8

Luximon-Ramma, A., Bahorun, T., Soobrattee, MA., &Aruoma, OI. Antioxidant activities of phenolic, proanthocyanidin, and flavonoid components in extracts of Cassia fistula. J Agric Food Chem. 2002;50: 5042-5047. https://doi.org/10.1021/jf0201172
Kurt S, Koca RH, Hürkul MM, Seker U, Köroğlu A. The antioxidant effect of Michauxia campanuloides on rat ovaries. J Hellenic Vet Med Soci. 2021;72(3): 3163-3170. https://doi.org/10.12681/jhvms.28510
Rajasekaran S, Sivagnanam K, Subramanian S. Antioxidant effect of Aloe vera gel extract in streptozotocin-induced diabetes in rats. Pharmacol Rep. 2005;57: 90-96.
Anilakumar KR, Sudarshanakrishna KR, Chandramohan G, Ilaiyaraja N, Khanum F, Bawa AS. Effect of Aloe vera gel extract on antioxidant enzymes and azoxymethane-induced oxidative stress in rats. Indian Journal of Experimental Biology. 2010;48: 837-842.
Manvitha K, Bidya B. Aloe vera: a wonder plant its history, cultivation and medicinal uses. J Pharmacogn Phytochem 2014;2: 85-88.
Eshun K, He Q. Aloe vera: a valuable ingredient for the food, pharmaceutical and cosmetic industries—a review. Critical reviews in food science and nutrition. 2004;44(2): 91-96. https://doi.org/10.1080/10408690490424694
Cristiano G, Murillo-Amador B, De Lucia B. Propagation techniques and agronomic requirements for the cultivation of Barbados Aloe (Aloe vera (L.) Burm. F.) - a review. Front Plant Sci. 2016;7: 1410. https://doi.org/10.3389/fpls.2016.01410
Tan K. Aloe vera (L.) Burm.f. In: Davis PH (Ed.). Flora of Turkey and the East Aegean Islands. Edinburgh UniversityPress, Edinburgh, 1984;8: 355-356.
Maan AA, Nazir A, Khan MKI, Ahmad T, Zia R, Murid M, Abrar M. The therapeutic properties and applications of Aloe vera: A review J Herb Med. 2018;12: 1-10https://doi.org/10.1016/j.hermed.2018.01.002
Majumder R, Das CK, Mandal M. Lead bioactive compounds of Aloe vera as potential anticancer agent. Pharmacol Res 2019;148: 104416. https://doi.org/10.1016/j.phrs.2019.104416
Kumar, S. and Yadav, J.P. Ethnobotanical and pharmacological properties of Aloe vera: a review. J Med Plant Res. 2014;8: 1387-1398.
Manjunath K, BhanuPrakash G, Subash KR, Tadvi NA, Manikanta M, UmamaheswaraRao K. Effect of Aloe vera leaf extract on blood glucose levels in alloxan induced diabetic rats. Natl J Physiol Pharm. 2016;6: 471-474. https://doi.org/10.5455/njppp.2016.6.0718613072016
Haritha K, Ramesh B, Saralakumari D. Effect of Aloe vera gel on antioxidant enzymes in streptozotocin-induced cataractogenesis in male and female Wistar rats. Journal of Acute Medicine. 2014;4: 38-44. https://doi.org/10.1016/j.jacme.2014.01.005
Park MK, Park JH, Kim NY, Shin YG, Choi YS, Lee JG, Lee SK. Analysis of 13 phenolic compounds in Aloe species by high performance liquid chromatography. Phytochemical Analysis: Int J Plant Chem Biochem Technol. 1998;9: 186- 191. https://doi.org/10.1002/(SICI)1099-1565(199807/08)9:4%3c186::AID-PCA406%3e3.0.CO;2-%23
International Conference on Harmonization of Technical Requirements for Registration of Pharmaceuticals for Human Use. Validation of Analytical Procedures: Text and Methodology Q2 (R1), 2005; 1-13.
Chabrolle C, JeanPierre E, Tosca L, Ramé C, Dupont J. Effects of high levels of glucose on the steroidogenesis and the expression of adiponectin receptors in rat ovarian cells. Reproductive Biology and Endocrinology 2008; 6(1): 1-14. https://doi.org/10.1186/1477-7827-6-11
Ghanbari E, Nejati V, Khazaei M. Improvement in serum biochemical alterations and oxidative stress of liver and pancreas following use of royal jelly in streptozotocin-induced diabetic rats. Cell Journal (Yakhteh) 2016;18(3): 362. https://doi.org/10.22074/cellj.2016.4564
Lalier L, Cartron PF, Juin P, Nedelkina S, Manon S, Bechinger B, Vallette FM. Bax activation and mitochondrial insertion during apoptosis. Apoptosis 2007;12: 887-896. https://doi.org/10.1007/s10495-007-0749-1
Annibaldi A, Meier P. Checkpoints in TNF-induced cell death: implications in inflammation and cancer. Trends in molecular medicine 2018;24(1): 49-65. https://doi.org/10.1016/j.molmed.2017.11.002
Strzalka W, Ziemienowicz A. Proliferating cell nuclear antigen (PCNA): a keyfactor in DNA replication and cell cycle regulation. Ann Bot. 2011;107: 1127-1140. https://doi.org/10.1093/aob/mcq243
TomĂĄnek M, Chronowska E. Immunohistochemical localization of proliferating cell nuclear antigen (PCNA) in the pig ovary. Folia Histochem Cyto. 2006;44: 269-274.
Can B, Atilgan R, Pala S, Kuloğlu T, Kiray S, Ilhan N. Examination of the effect of ovarian radiation injury induced by hysterosalpingography on ovarian proliferating cell nuclear antigen and the radioprotective effect of amifostine: an experimental study. Drug design, development and therapy. 2018;12: 1491.https://doi.org/10.2147/DDDT.S156757
Sugino N. Reactive oxygen species in ovarian physiology. Reprod Med Biol. 2005;4: 31-44. https://doi.org/10.1111/j.1447-0578.2005.00086.x
Rajaei S, Alihemmati A, Abedelahi A. Antioxidant effect of genistein on ovarian tissue morphology, oxidant and antioxidant activity in rats with induced polycystic ovary syndrome. Int J Rep Biomed. 2019;17: 1-12. https://doi.org/10.18502/ijrm.v17i1.3816
Pokorný J. Are natural antioxidants better–and safer–than synthetic antioxidants?.European journal of lipid science and technology. 2007; 109(6): 629-642.https://doi.org/10.1002/ejlt.200700064
Asif M. Chemistry and antioxidant activity of plants containing some phenolic compounds. Chemistry international. 215;1(1): 35-52.
Soleymanı MM, Nourafshan A, Hamta A, Moumenı HR, Abnousi MH, Mahmoudi M, Anvari M, Hazaveh M. Effects of vitamin E on ovarian tissue of rats following treatment with p-nonylphenol. A Stereological Study. Iran J Reprod Med. 2010;8, 1-9.
Majd NE, Azizian H, Tabandeh MR, Shahriari A. Effect of Abelmoschus esculentus powder on ovarian histology, expression of apoptotic genes and oxidative stress in diabetic rats fed with high fat diet. IJPR. 2019;18: 369-382.
Nna VU, Usman UZ, Ofutet EO, Owu DU. Quercetin exerts preventive, ameliorative and prophylactic effects on cadmium chloride-induced oxidative stress in the uterus and ovaries of female Wistar rats. Food Chem Toxicol. 2017;102: 143-155.https://doi.org/10.1016/j.fct.2017.02.010
Shathili AM, Brown HM, Everest-Dass AV, Tan TC, Parker LM, Thompson JG, Packer NH. The effect of streptozotocin-induced hyperglycemia on N-and O-linked protein glycosylation in mouse ovary. Glycobiology. 2018;28: 832-840.https://doi.org/10.1093/glycob/cwy075
Drimal J, Knezl V, Navarova J, Nedelcevova J, Paulovicova E, Sotnikova R, Drimal D. Role of inflammatory cytokines and chemoattractants in the rat model of streptozotocin-induced diabetic heart failure. Endocrine regulations. 2008;42(4): 129-135.
shkar F, Rezaei S, Salahshoornezhad S, Vahid F, Gholamalizadeh M, Dahka SM, Doaei S. The Role of medicinal herbs in treatment of insulin resistance in patients with Polycystic Ovary Syndrome: A literature review. Biomolecular concepts 2019;11(1): 57-75.
Rajasekaran S, Sivagnanam K, Ravi K, Subramanian S. Hypoglycemic effect of Aloe vera gel on streptozotocin- induced diabetes in experimental rats. Journal of Medicinal food. 2004;7(1): 61-66. https://doi.org/10.1089/109662004322984725
Rajasekaran S, Sivagnanam K, Subramanian S. Modulatory effects of Aloe vera leaf gel extract on oxidative stress in rats treated with streptozotocin. Journal of pharmacy and pharmacology 2005;57(2): 241-246. https://doi.org/10.1211/0022357055416
Sakr SA, Abdel-Aziz KK, El-kott AF, Khalifa HS. Ameliorative effect of olive leaves extract on hepatotoxicity and oxidative stress in streptozotocin-induced diabetic rats. JBAR. 2016;2, 549-560. https://doi.org/10.21608/JBAAR.2016.108933
Feldman AT, Wolfe D. (2014). Tissue processing and hematoxylin and eosinstaining. In Histopathology. New York: Humana Press; 2014. p.31-43. https://doi.org/10.1007/978-1-4939-1050-2_3
Seker U, Eşki F, Kurt S. Investigation of the Distribution and Expression Level of Pro and Anti-Apoptotic Bax and Bcl-2 in Ovarian Follicles at DifferentDevelopmentalStages. Harran Üniversitesi Tıp Fakültesi Dergisi. 2021; 18: 424-428. https://doi.org/10.35440/hutfd.995512
Eşki F, Kurt S, Demir PA. Effect of different estrus synchronization protocols on estrus and pregnancy rates, oxidative stress and some biochemical parameters in Hair goats. Small Ruminant Research. 2021;198: 1-6. https://doi.org/10.1016/j.smallrumres.2021.106348
Nilsson EE, Kezele P, Skinner MK. Leukemia inhibitory factor (LIF) promotes the primordial to primary follicle transition in rat ovaries. Mol Cell Endocrinol. 2002;188: 65-73. https://doi.org/10.1016/S0303-7207(01)00746-8
Muskhelishvili L, Wingard SK, Latendresse JR. Proliferating cell nuclear antigen—a marker for ovarian follicle counts. Toxicol Pathol 2005;33: 365-368. https://doi.org/10.1080/01926230590930164

Details

Primary Language

English

Subjects

Pharmaceutical Botany

Journal Section

Research Article

Authors

Serdal Kurt ^*
0000-0002-0191-3245
Türkiye

Uğur Şeker
Türkiye

Murat Onur Yazlık
0000-0002-0039-5597
Türkiye

Engin Er This is me
0000-0002-7713-3704
Türkiye

Muhammed Mesud Hürkul
0000-0002-9241-2496
Türkiye

Sevda Söker
0000-0001-6453-0033
Türkiye

Publication Date

June 27, 2025

Submission Date

August 25, 2022

Acceptance Date

December 8, 2022

Published in Issue

Year 2023 Volume: 27 Number: 2

IZ

https://izlik.org/JA49MS52WP

Cite

RIS / Bibtex

APA

Kurt, S., Şeker, U., Yazlık, M. O., Er, E., Hürkul, M. M., & Söker, S. (2025). Identification of major phenolic compounds of Aloe vera and its protective effect on ovaries under oxidative stress in diabetic rats. Journal of Research in Pharmacy, 27(2), 652-664. https://izlik.org/JA49MS52WP

AMA

1.Kurt S, Şeker U, Yazlık MO, Er E, Hürkul MM, Söker S. Identification of major phenolic compounds of Aloe vera and its protective effect on ovaries under oxidative stress in diabetic rats. J. Res. Pharm. 2025;27(2):652-664. https://izlik.org/JA49MS52WP

Chicago

Kurt, Serdal, Uğur Şeker, Murat Onur Yazlık, Engin Er, Muhammed Mesud Hürkul, and Sevda Söker. 2025. “Identification of Major Phenolic Compounds of Aloe Vera and Its Protective Effect on Ovaries under Oxidative Stress in Diabetic Rats”. Journal of Research in Pharmacy 27 (2): 652-64. https://izlik.org/JA49MS52WP.

EndNote

Kurt S, Şeker U, Yazlık MO, Er E, Hürkul MM, Söker S (June 1, 2025) Identification of major phenolic compounds of Aloe vera and its protective effect on ovaries under oxidative stress in diabetic rats. Journal of Research in Pharmacy 27 2 652–664.

IEEE

[1]S. Kurt, U. Şeker, M. O. Yazlık, E. Er, M. M. Hürkul, and S. Söker, “Identification of major phenolic compounds of Aloe vera and its protective effect on ovaries under oxidative stress in diabetic rats”, J. Res. Pharm., vol. 27, no. 2, pp. 652–664, June 2025, [Online]. Available: https://izlik.org/JA49MS52WP

ISNAD

Kurt, Serdal - Şeker, Uğur - Yazlık, Murat Onur - Er, Engin - Hürkul, Muhammed Mesud - Söker, Sevda. “Identification of Major Phenolic Compounds of Aloe Vera and Its Protective Effect on Ovaries under Oxidative Stress in Diabetic Rats”. Journal of Research in Pharmacy 27/2 (June 1, 2025): 652-664. https://izlik.org/JA49MS52WP.

JAMA

1.Kurt S, Şeker U, Yazlık MO, Er E, Hürkul MM, Söker S. Identification of major phenolic compounds of Aloe vera and its protective effect on ovaries under oxidative stress in diabetic rats. J. Res. Pharm. 2025;27:652–664.

MLA

Kurt, Serdal, et al. “Identification of Major Phenolic Compounds of Aloe Vera and Its Protective Effect on Ovaries under Oxidative Stress in Diabetic Rats”. Journal of Research in Pharmacy, vol. 27, no. 2, June 2025, pp. 652-64, https://izlik.org/JA49MS52WP.

Vancouver

1.Serdal Kurt, Uğur Şeker, Murat Onur Yazlık, Engin Er, Muhammed Mesud Hürkul, Sevda Söker. Identification of major phenolic compounds of Aloe vera and its protective effect on ovaries under oxidative stress in diabetic rats. J. Res. Pharm. [Internet]. 2025 Jun. 1;27(2):652-64. Available from: https://izlik.org/JA49MS52WP