Identification of major phenolic compounds of Aloe vera and its protective effect on ovaries under oxidative stress in diabetic rats

Year 2023, Volume: 27 Issue: 2, 652 - 664, 27.06.2025

Serdal Kurt , Uğur Şeker , Murat Onur Yazlık , Engin Er Muhammed Mesud Hürkul , Sevda Söker

https://izlik.org/JA49MS52WP

Abstract

The study investigated the major phenolic compounds of Aloe vera leaf (AVL) by the high-performance
liquid chromatography (HPLC) method, and its protective effect on ovaries under oxidative stress of streptozotocin-
induced diabetic rats. The study was conducted on control (untreated healthy rats; C), diabetes (untreated diabetic rats;
D) and diabetes+A. vera treatment (diabetic rats treated with A. vera; D+A) groups. D+A group was given an ethanolic
extract of A. vera (300 mg/kg) for 14 days. Major phenolic compounds of AVL were chlorogenic acid and rutin. alanine
transaminase (ALT) and aspartate aminotransferase (AST) levels increased in D group compared to others groups
(P<0.01). Alkaline phosphatase (ALP) and albumin levels were lower and higher in the C group than in other groups,
respectively (P<0.01). In the D group compared to other groups, oxidative stress index (OSI) and total oxidant status
levels increased, while total antioxidant status level decreased in both ovarian tissues and blood (P<0.01). Preantral and
antral follicle counts decreased in the D group according to other groups (P<0.01) and atretic follicle count increased
(P<0.05). Proliferating cell nuclear antigen (PCNA) expression level (P<0.01), and B-cell lymphoma-2-associated-X-
protein (Bax; P<0.01) and tumor necrosis factor-alpha (TNF-α) expression levels (P<0.05) decreased and increased in
group D according to other groups, respectively. In conclusion, treatment with AVL decreased OSI, improved follicular
dynamics, and restored Bax, TNF-α and PCNA expression in diabetic rats.

Keywords

Aloe vera , antioxidant , diabetes , HPLC , ovarian , rat

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