The cytotoxic effect of p(SweetAlmondOil) and p(JuniperOil) organo-particles on L-929 and Capan-1 cells line

Duygu Alpaslan; Abdullah Turan; Tuba Erşen Dudu; Büşra Moran; Nahit Aktaş; Mustafa Türk

The cytotoxic effect of p(SweetAlmondOil) and p(JuniperOil) organo-particles on L-929 and Capan-1 cells line

Abstract

Abstract Within the scope of the presented study, p(SAO) and p(JO) organo-particles were synthesized from Sweet almond oil and Juniper oil by redox polymerization method to be used in cancer immunotherapy and their characterization studies were carried out. Then, MTT analysis was performed on L-929 fibroblast and Capan-1 pancreatic cancer cell line via synthesized p(SAO) and p(JO) organo-particles The chemical structure and functional groups of p(SAO) and p(JO) organo-particles were determined by Fourier Transform Infrared Spectroscopy (FT-IR). Organo particle size, zeta potential values and size distribution were determined by the dynamic light scattering (DLS) method using Zetasizer device. The morphological features of the particles were determined by scanning electron microscopy (SEM). Bioactivity properties were determined by antioxidant, antimicrobial and biocompatibility analyses. As a result of MTT analysis on L-929 fibroblast and Capan-1 pancreatic cancer cell line through synthesized p(SAO) and p(JO) organo-particles It was determined that p(SAO) and p(JO) organo-particles increased cell viability in fibroblast cells. The lethal effect of p(JO) on Capan-1 pancreatic cancer cells was found to be close to doxorubicin. Observed that the use of p(JO) together with doxorubicin will lead to better results than the use of doxorubicin alone.

Keywords

References

[1] Ali, B.; Al-Wabel, N. A.; Shams, S.; Ahamad, A.; Khan, S. A.; Anwar, F. Essential oils used in aromatherapy: A systemic review. Asian Pacific Journal of Tropical Biomedicine. 2015; 5(8): 601-611. https://doi.org/10.1016/j.apjtb.2015.05.007
[2] Wayteck, L.; Breckpot, K.; Demeester, J.; Smedt, S. C. D.; Raemdonck, K. A personalized view on cancer immunotherapy. Cancer Letters. 2014; 352(1): 12.
[3] Szczerbanik, M.; Jobling, J.; Morris, S.; Holford, S. Essential oil vapours control some common postharvest fungal pathogens. Australian Journal of Experimental Agriculture. 2007; 47: 6.
[4] Leyva-Lopez, N.; Gutierrez-Grijalva, E. P.; Vazquez-Olivo, G.; Heredia, J. B. Essential Oils of Oregano: Biological Activity beyond Their Antimicrobial Properties. Molecules. 2017; 22(6): https://doi.org/10.3390/molecules22060989
[5] Lizarraga-Valderrama, L. R. Effects of essential oils on central nervous system: Focus on mental health. Phytother Res. 2021; 35(2): 657-679. https://doi.org/10.1002/ptr.6854
[6] Saad, N. Y.; Muller, C. D.; Lobstein, A. Major bioactivities and mechanism of action of essential oils and their components. Flavour and Fragrance Journal. 2013; 28(5): 269-279. https://doi.org/10.1002/ffj.3165
[7] Zhou, J. Advances and Prospects in Cancer Immunotherapy. New Journal of Science. 2014; 2014: 1-13. https://doi.org/10.1155/2014/745808
[8] Oliveira, P. F. d.; Alves, J. M.; Damasceno, J. L.; Oliveira, R. A. M.; Dias, H. J.; Crotti, A. E. M.; Tavares, D. C. Cytotoxicity screening of essential oils in cancer cell lines. Revista Brasileira de Farmacognosia. 2015; 25(2): 183-188. https://doi.org/10.1016/j.bjp.2015.02.009

[9] Tak, J.-H.; Jovel, E.; Isman, M. B. Contact, fumigant, and cytotoxic activities of thyme and lemongrass essential oils against larvae and an ovarian cell line of the cabbage looper, Trichoplusia ni. Journal of Pest Science. 2015; 89(1): 183-193. https://doi.org/10.1007/s10340-015-0655-1
[10] Alpaslan, D.; Turan, A.; Ersen Dudu, T.; Moran Bozer, B.; Aktas, N.; Turk, M. Characterization of p(PmO), p(LO) and p(RO) organoparticles, their bioactivity properties and their effect on pancreatic cancer Capan-1 cell. Materials Chemistry and Physics. 2023; 127871. https://doi.org/10.1016/j.matchemphys.2023.127871
[11] Alpaslan, D.; Turan, A.; Ersen Dudu, T.; Aktas, N. Properties, Bioactivity, In vitro Drug Release and Functionality as a Biomaterial of Poly (Apigenin) Hydrogel. Advances ın Pharmacology and Pharmacy. 2025; 13(3): 323-332. https://doi.org/10.13189/app.2025.130304
[12] Olak, T.; Turan, A.; Alpaslan, D.; Ersen Dudu, T.; Aktas, N. Developing poly(agarco-glycerol-co-thyme oil) based organo-hydrogels for the controlled drug release applications. Journal of drug delıvery scıence and technology. 2020; 60(2020): 102088. https://doi.org/10.1016/j.jddst.2020.102088
[13] Alpaslan, D.; Ersen Dudu, T.; Moran Bozer, B.; Aktas, N.; Turk, M. p(thyme oil) and p(clove oil) organo-particles with biocompatible, anticancer, antioxidant, and antibacterial properties against Capan-1 and L-929 cells. The Canadian Journal of Chemical Engineering 2023; 1: 12. https://doi.org/10.1002/cjce.25061
[14] Alpaslan, D.; Olak, T.; Turan, A.; Ersen Dudu, T.; Aktas, N. A garlic oil-based organo-hydrogel for use in ph-sensitive drug release. Chemical papers. 2021; 75(11): 5759-5772. https://doi.org/10.1007/s11696-021-01760-2
[15] Sapsford, K. E.; Tyner, K. M.; Dair, B. J.; Deschamps, J. R.; Medintz, I. L. Analyzing nanomaterial bioconjugates: a review of current and emerging purification and characterization techniques. Anal Chem. 2011; 83(12): 4453-4488. https://doi.org/10.1021/ac200853a
[16] Degen, A.; Kosec, M. Effect of pH and impurities on the surface charge of zinc oxide in aqueous solution. Journal of the European Ceramic Society. 2000; 20: 6.
[17] Thygesen, L. G.; Løkke, M. M.; Micklander, E.; Engelsen, S. B. Vibrational microspectroscopy of food. Raman vs. FT-IR. Trends in Food Science & Technology. 2003; 14(1-2): 50-57. https://doi.org/10.1016/s0924-2244(02)00243-1
[18] Ersen Dudu, T.; Alpaslan, D.; Aktas, N. Application of Poly (Agar-Co-Glycerol-Co-Sweet Almond Oil) Based Organo-Hydrogels as a Drug Delivery Material. J Polym Environ. 2021; 30(2): 483-493. https://doi.org/10.1007/s10924-021-02212-4
[19] Rodríguez-Maecker, R.; Vyhmeister, E.; Meisen, S.; Rosales Martinez, A.; Kuklya, A.; Telgheder, U. Identification of terpenes and essential oils by means of static headspace gas chromatography-ion mobility spectrometry. Analytical and Bioanalytical Chemistry. 2017; 409(28): 6595-6603. https://doi.org/10.1007/s00216-017-0613-2
[20] Ozcan, M. M.; Matthaus, B.; Aljuhaimi, F.; Mohamed Ahmed, I. A.; Ghafoor, K.; Babiker, E. E.; Osman, M. A.; Gassem, M. A.; Alqah, H. A. S. Effect of almond genotypes on fatty acid composition, tocopherols and mineral contents and bioactive properties of sweet almond (Prunus amygdalus Batsch spp. dulce) kernel and oils. J Food Sci Technol. 2020; 57(11): 4182-4192. https://doi.org/10.1007/s13197-020-04456-9
[21] Melhaoui, R.; Kodad, S.; Houmy, N.; Belhaj, K.; Mansouri, F.; Abid, M.; Addi, M.; Mihamou, A.; Sindic, M.; Serghini-Caid, H.; et al. Characterization of Sweet Almond Oil Content of Four European Cultivars (Ferragnes, Ferraduel, Fournat, and Marcona) Recently Introduced in Morocco. Scientifica (Cairo). 2021; 2021: 9141695. https://doi.org/10.1155/2021/9141695
[22] Alpaslan, D.; Dudu, T. E.; Moran Bozer, B.; Aktas, N.; Turk, M. Poly(CnO) and Poly(CcO) Organo-Particles Produced from Coconut Oil (CnO) and Cocoa Oil (CcO): Synthesis, Characterization, Bio and Anticancer Activity. Adv Pharmacol Pharm 2025, 13 (2), 18.
[23] Brigger, I.; Dubernet, C.; Couvreur, P. Nanoparticles in cancer therapy and diagnosis. Advanced Drug Delivery Reviews. 2012; 64: 24-36. https://doi.org/10.1016/j.addr.2012.09.006
[24] Hakan, Ç.; Türk, M.; Tamer, U.; Karahan, S.; Menemen, Y. Silver nanoparticles: cytotoxic, apoptotic, and necrotic effects on MCF-7 cells. Turkish Journal of Biology. 2013; 37: 573-581. https://doi.org/10.3906/biy-1302-21
[25] Silva, A. C. R. d.; Lopes, P. M.; Azevedo, M. M. B. d.; Costa, D. C. M.; Alviano, C. S.; Alviano, D. S. Biological activities of α-pinene and β-pinene. Molecules. 2012; 17(6): 11.
[26] Salehi, B.; Upadhyay, S.; Orhan, I. E.; Jugran, A. K.; Jayaweera, S. L. D.; Dias, D. A.; Sharopov, F.; Taheri, Y.; Martins, N.; Baghalpour, N.; et al. Therapeutic Potential of α- and β-Pinene. Biomolecules. 2019; 9(11).
[27] Miguel, M. G. Antioxidant and anti-inflammatory activities of essential oils. Molecules 2010; 15(12): 35.
[28] Dhifi, W.; Bellili, S.; Jazi, S.; Bahloul, N.; Mnif, W. Essential Oils’ Chemical Characterization and Investigation of Some Biological Activities: A Critical Review. Medicines. 2016; 3(4): 25.
[29] Alpaslan, D.; Olak, T.; Turan, A.; Dudu, T. E.; Aktas, N. Use of coconut oil-based organo-hydrogels in pharmaceutical applications. Journal of Polymers and the Environment 2022; 30(2): 666-680. https://doi.org/10.1007/s10924-021-02219-x

Details

Primary Language

English

Subjects

Pharmaceutical Chemistry, Basic Pharmacology

Journal Section

Research Article

Authors

Duygu Alpaslan ^*
0000-0002-6007-3397
Türkiye

Abdullah Turan
0000-0003-3330-4468
Türkiye

Tuba Erşen Dudu
0000-0001-5564-2834
Türkiye

Büşra Moran
0000-0002-7280-4417
Türkiye

Nahit Aktaş
0000-0001-9341-607X
Türkiye

Mustafa Türk
0000-0001-8202-090X
Türkiye

Publication Date

August 3, 2026

Submission Date

June 17, 2025

Acceptance Date

July 6, 2026

Published in Issue

Year 2026 Volume: 30 Number: 4

IZ

https://izlik.org/JA39KK27TE

Cite

RIS / Bibtex

APA

Alpaslan, D., Turan, A., Erşen Dudu, T., Moran, B., Aktaş, N., & Türk, M. (2026). The cytotoxic effect of p(SweetAlmondOil) and p(JuniperOil) organo-particles on L-929 and Capan-1 cells line. Journal of Research in Pharmacy, 30(4), 1027-1044. https://izlik.org/JA39KK27TE

AMA

1.Alpaslan D, Turan A, Erşen Dudu T, Moran B, Aktaş N, Türk M. The cytotoxic effect of p(SweetAlmondOil) and p(JuniperOil) organo-particles on L-929 and Capan-1 cells line. J. Res. Pharm. 2026;30(4):1027-1044. https://izlik.org/JA39KK27TE

Chicago

Alpaslan, Duygu, Abdullah Turan, Tuba Erşen Dudu, Büşra Moran, Nahit Aktaş, and Mustafa Türk. 2026. “The Cytotoxic Effect of P(SweetAlmondOil) and P(JuniperOil) Organo-Particles on L-929 and Capan-1 Cells Line”. Journal of Research in Pharmacy 30 (4): 1027-44. https://izlik.org/JA39KK27TE.

EndNote

Alpaslan D, Turan A, Erşen Dudu T, Moran B, Aktaş N, Türk M (August 1, 2026) The cytotoxic effect of p(SweetAlmondOil) and p(JuniperOil) organo-particles on L-929 and Capan-1 cells line. Journal of Research in Pharmacy 30 4 1027–1044.

IEEE

[1]D. Alpaslan, A. Turan, T. Erşen Dudu, B. Moran, N. Aktaş, and M. Türk, “The cytotoxic effect of p(SweetAlmondOil) and p(JuniperOil) organo-particles on L-929 and Capan-1 cells line”, J. Res. Pharm., vol. 30, no. 4, pp. 1027–1044, Aug. 2026, [Online]. Available: https://izlik.org/JA39KK27TE

ISNAD

Alpaslan, Duygu - Turan, Abdullah - Erşen Dudu, Tuba - Moran, Büşra - Aktaş, Nahit - Türk, Mustafa. “The Cytotoxic Effect of P(SweetAlmondOil) and P(JuniperOil) Organo-Particles on L-929 and Capan-1 Cells Line”. Journal of Research in Pharmacy 30/4 (August 1, 2026): 1027-1044. https://izlik.org/JA39KK27TE.

JAMA

1.Alpaslan D, Turan A, Erşen Dudu T, Moran B, Aktaş N, Türk M. The cytotoxic effect of p(SweetAlmondOil) and p(JuniperOil) organo-particles on L-929 and Capan-1 cells line. J. Res. Pharm. 2026;30:1027–1044.

MLA

Alpaslan, Duygu, et al. “The Cytotoxic Effect of P(SweetAlmondOil) and P(JuniperOil) Organo-Particles on L-929 and Capan-1 Cells Line”. Journal of Research in Pharmacy, vol. 30, no. 4, Aug. 2026, pp. 1027-44, https://izlik.org/JA39KK27TE.

Vancouver

1.Duygu Alpaslan, Abdullah Turan, Tuba Erşen Dudu, Büşra Moran, Nahit Aktaş, Mustafa Türk. The cytotoxic effect of p(SweetAlmondOil) and p(JuniperOil) organo-particles on L-929 and Capan-1 cells line. J. Res. Pharm. [Internet]. 2026 Aug. 1;30(4):1027-44. Available from: https://izlik.org/JA39KK27TE