Determination of the antimicrobial and antibiofilm activity of lyophilized cornelian cherry (Cornus mas L.)

Year 2025, Volume: 29 Issue: 4, 1542 - 1550, 05.07.2025

Zehra Margot Çelik , Kadriye Kurt , Erkan Rayaman , Miraç Albayrak , Oktay Akpınar , Gizem Emre

https://doi.org/10.12991/jrespharm.1734691

https://izlik.org/JA86GP95NT

Abstract

This study investigates the antimicrobial and antibiofilm activities of lyophilized Cornelian cherry (Cornus
mas L.) and its methanol extract against a range of clinically significant pathogens. The research aimed to evaluate the
potential of these natural compounds as alternative antimicrobial agents, given the global rise in antimicrobial
resistance. Antibacterial activity was assessed using agar well diffusion and broth microdilution methods, while
antibiofilm efficacy was determined through biofilm inhibition assays. The methanol extract exhibited broad-spectrum
antibacterial activity, effectively inhibiting all tested bacterial strains, whereas the lyophilized form demonstrated no
activity against Klebsiella pneumoniae and Escherichia coli. Both forms showed strong biofilm inhibition, particularly
against Staphylococcus aureus, Enterococcus faecalis, Streptococcus pyogenes, Pseudomonas aeruginosa, and Acinetobacter
baumannii, with inhibition increasing in a dose-dependent manner and peaking at the minimum inhibitory
concentration. No antifungal activity was observed against the tested Candida species. The superior efficacy of the
methanol extract may be attributed to a higher concentration of phenolic and anthocyanin compounds. These findings
suggest that Cornelian cherry, particularly in its methanol-extracted form, holds promise as a natural antimicrobial and
antibiofilm agent. Further research is warranted to explore its potential applications in clinical and industrial settings.

Keywords

Cornelian cherry , Cormus mas L. , antimicrobial effect , antibiofilm effect

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