Hepatoprotective and Antioxidant Properties of Pressure-Blanched Curcuma xanthorrhiza Roxb. Powder in CCl4-treated HepG2 Cell

Dwiyati Pujimulyani; Bayu Kanetro; Audrey Amira Crystalia; Sulkhan Windrayahya; Queen Saint Monica Vannessa Liu; David Chandra Liauwan Makpal; Shereen Angelina Rattu; Steve Anthonius Lefrand Makalew; Andrea Dhieta Utama

doi:10.12991/jrespharm.1881565

Hepatoprotective and Antioxidant Properties of Pressure-Blanched Curcuma xanthorrhiza Roxb. Powder in CCl4-treated HepG2 Cell

Abstract

Introduction: Oxidative stress plays a key role in liver diseases such as CCl4-induced injury, highlighting the therapeutic potential of natural antioxidants. Curcuma xanthorrhiza Roxb. (Javanese turmeric), rich in curcuminoids and xanthorrhizol, possesses antioxidant properties. Processing affects bioactive compounds; blanching can preserve them by inactivating enzymes. This study evaluated the antioxidant and hepatoprotective effects of pressure-blanched C. xanthorrhiza powder, processed via different drying methods, against CCl4-induced injury in HepG2 cells. Methods: C. xanthorrhiza rhizomes were pressure-blanched (120 °C/28.81 psia, 0-10 min), then dried (cabinet at 50-55 °C or sun). Powdered ethanol extracts were assessed for antioxidant activity (DPPH, FRAP), total phenolics, flavonoids, curcuminoids, and tannins. Hepatoprotective effect was measured in CCl4-treated HepG2 cells via MTS assay (viability) and AST activity. Results: Both drying methods yielded powder extracts with significant antioxidant activity. Sun-drying showed higher DPPH scavenging; FRAP was optimal after 5 min of blanching for both. Bioactive compound levels varied based on blanching time and drying. Extracts protected HepG2 cells from CCl4 toxicity, improving viability and reducing AST. Discussion: Pressure blanching (especially 5 min) and appropriate drying effectively preserved and enhanced C. xanthorrhiza's antioxidant and hepatoprotective properties. The protection against CCl4-induced injury in HepG2 cells is likely due to high antioxidant capacity and preserved bioactive compounds. These findings highlight pressure-blanched C. xanthorrhiza's potential for liver health, warranting in vivo studies.

Keywords

Project Number

272/B.02/H1/VI/2023

Thanks

Expressıons of gratıtude for the research collaboratıon between the Indonesia International Institute for Life Sciences and Mercu Buana Unıversıty Yogyakarta. Thıs research receıved research fundıng from Kemenristekdikti with number contact: 272/B.02/H1/VI/2023, which funded the research until its completion. Thank’s to Dr. Nuralih (Research Centerfor Pharmaceutical Ingredients and Traditional Medicine, National Research and Innovation Agency (BRIN)) for helping to obtain cell culture materials

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Details

Primary Language

English

Subjects

Pharmacology and Pharmaceutical Sciences (Other)

Journal Section

Research Article

Authors

Dwiyati Pujimulyani ^*
0000-0002-8175-5653
Indonesia

Bayu Kanetro This is me
0000-0002-4522-5575
Indonesia

Audrey Amira Crystalia This is me
0009-0002-7869-5995
Indonesia

Sulkhan Windrayahya This is me
0000-0002-6634-4821
Indonesia

Queen Saint Monica Vannessa Liu This is me
Indonesia

David Chandra Liauwan Makpal This is me
0009-0004-8583-2021
Indonesia

Shereen Angelina Rattu This is me
0009-0002-0813-2844
Indonesia

Steve Anthonius Lefrand Makalew This is me
0009-0005-8424-8760
Indonesia

Andrea Dhieta Utama This is me
Indonesia

Publication Date

October 6, 2026

Submission Date

March 11, 2026

Acceptance Date

September 28, 2026

Published in Issue

Year 2026 Volume: 30 Number: 5

DOI

https://doi.org/10.12991/jrespharm.1881565

IZ

https://izlik.org/JA92SU42ZD

Cite

RIS / Bibtex

APA

Pujimulyani, D., Kanetro, B., Crystalia, A. A., Windrayahya, S., Liu, Q. S. M. V., Makpal, D. C. L., Rattu, S. A., Makalew, S. A. L., & Utama, A. D. (2026). Hepatoprotective and Antioxidant Properties of Pressure-Blanched Curcuma xanthorrhiza Roxb. Powder in CCl4-treated HepG2 Cell. Journal of Research in Pharmacy, 30(5), 1378-1395. https://doi.org/10.12991/jrespharm.1881565

AMA

1.Pujimulyani D, Kanetro B, Crystalia AA, et al. Hepatoprotective and Antioxidant Properties of Pressure-Blanched Curcuma xanthorrhiza Roxb. Powder in CCl4-treated HepG2 Cell. J. Res. Pharm. 2026;30(5):1378-1395. doi:10.12991/jrespharm.1881565

Chicago

Pujimulyani, Dwiyati, Bayu Kanetro, Audrey Amira Crystalia, et al. 2026. “Hepatoprotective and Antioxidant Properties of Pressure-Blanched Curcuma Xanthorrhiza Roxb. Powder in CCl4-Treated HepG2 Cell”. Journal of Research in Pharmacy 30 (5): 1378-95. https://doi.org/10.12991/jrespharm.1881565.

EndNote

Pujimulyani D, Kanetro B, Crystalia AA, Windrayahya S, Liu QSMV, Makpal DCL, Rattu SA, Makalew SAL, Utama AD (October 1, 2026) Hepatoprotective and Antioxidant Properties of Pressure-Blanched Curcuma xanthorrhiza Roxb. Powder in CCl4-treated HepG2 Cell. Journal of Research in Pharmacy 30 5 1378–1395.

IEEE

[1]D. Pujimulyani et al., “Hepatoprotective and Antioxidant Properties of Pressure-Blanched Curcuma xanthorrhiza Roxb. Powder in CCl4-treated HepG2 Cell”, J. Res. Pharm., vol. 30, no. 5, pp. 1378–1395, Oct. 2026, doi: 10.12991/jrespharm.1881565.

ISNAD

Pujimulyani, Dwiyati - Kanetro, Bayu - Crystalia, Audrey Amira - Windrayahya, Sulkhan - Liu, Queen Saint Monica Vannessa - Makpal, David Chandra Liauwan - Rattu, Shereen Angelina - Makalew, Steve Anthonius Lefrand - Utama, Andrea Dhieta. “Hepatoprotective and Antioxidant Properties of Pressure-Blanched Curcuma Xanthorrhiza Roxb. Powder in CCl4-Treated HepG2 Cell”. Journal of Research in Pharmacy 30/5 (October 1, 2026): 1378-1395. https://doi.org/10.12991/jrespharm.1881565.

JAMA

1.Pujimulyani D, Kanetro B, Crystalia AA, Windrayahya S, Liu QSMV, Makpal DCL, Rattu SA, Makalew SAL, Utama AD. Hepatoprotective and Antioxidant Properties of Pressure-Blanched Curcuma xanthorrhiza Roxb. Powder in CCl4-treated HepG2 Cell. J. Res. Pharm. 2026;30:1378–1395.

MLA

Pujimulyani, Dwiyati, et al. “Hepatoprotective and Antioxidant Properties of Pressure-Blanched Curcuma Xanthorrhiza Roxb. Powder in CCl4-Treated HepG2 Cell”. Journal of Research in Pharmacy, vol. 30, no. 5, Oct. 2026, pp. 1378-95, doi:10.12991/jrespharm.1881565.

Vancouver

1.Dwiyati Pujimulyani, Bayu Kanetro, Audrey Amira Crystalia, Sulkhan Windrayahya, Queen Saint Monica Vannessa Liu, David Chandra Liauwan Makpal, Shereen Angelina Rattu, Steve Anthonius Lefrand Makalew, Andrea Dhieta Utama. Hepatoprotective and Antioxidant Properties of Pressure-Blanched Curcuma xanthorrhiza Roxb. Powder in CCl4-treated HepG2 Cell. J. Res. Pharm. 2026 Oct. 1;30(5):1378-95. doi:10.12991/jrespharm.1881565