Doğal Solunum Sinsitiyal Virüsü (RSV) ile Enfekte Koyun ve Keçi Akciğer Parafin Bloklarında TNF-a Ekspresyonunun İmmünohistokimyasal Olarak İncelenmesi

Abstract

Respiratuar Sinsitiyal Virüsü (RSV), koyun ve keçi solunum yolu hastalığının önemli bir nedenidir. RSV, genellikle solunum yolları epitelinde replike olur ve proinflamatuar sitokinler ve kemokinler indüklenir. Yapılan çalışmada, doğal RSV ile enfekte koyun ve keçi akciğer parafin bloklarında TNF-a ekspresyonu immunohistokimyasal yöntem ile araştırılması amaçlandı. Çalışma materyali, Etlik Veteriner Kontrol ve Araştırma Enstitüsüne, Ankara ve çevre illerinden 2015- 2020 yılları arasında pnömoni şüphesi ile gelen yirmi dokuz adet hasta (on dokuz Koyun ve on keçi) akciğer arşiv parafin blokları oluşturdu. Koyun ve keçi akciğer parafin blok dokularında bronş ve bronş epitelinde dejenerasyon ve deskuamasyon, fibromusküler hipertrofi, peribronşiyal lenfoid dokuda hiperplazi, interalveolar septumda hücre infiltrasyonu gibi histopatolojik bulgular istatistiksel olarak anlamlı değildi (p>0.05). İmmunohistaokimyasal olarak, bronş ve bronş epitelinde ve hücre döküntülerinde, bronş bezlerinde, interalveolar septum inflamatuar hücrelerde, alveolar makrofajlarda RSV replikasyonu koyun ve keçilerde istatiksel olarak benzerdi (p> 0.05). TNF-a’nın ekspresyonu keçilerin akciğer dokusunda koyunlara göre daha fazla şiddetli boyandığı (p<0.05) edildiği belirlendi. Sonuç olarak, koyun ve keçi sürülerinde solunum sistemi enfeksiyonlarında RSV enfeksiyonlarının önemli bir yer tuttuğu fark edildi. Koyun ve keçilerde doğal RSV enfeksiyonlarında sitokin fırtınasının tespit edilmesine yönelik çalışmaların yapılmasına, ayrıca günümüzde yeni bir tedavi yöntemi olan antikor tedavisinin koyun ve keçi viral enfeksiyonlarında araştırılması önerilmektedir.

Keywords

• RSV
• TNF-a
• İmmunohistokimya
• Koyun
• Keçi

Immunohistochemical Investigation of TNF-a Expression in Sheep and Goat Lung Paraffin Blocks Infected with Natural Respiratory Syncytial Virus (RSV)

Abstract

Respiratory Syncytial Virus (RSV) is an important cause of sheep and goat respiratory tract disease. RSV usually replicates in the airway epithelium and proinflammatory cytokines and chemokines are induced. In this study, it was aimed to investigate TNF-a expression in natural RSV-infected sheep and goat lung paraffin blocks by immunohistochemical method. The study material consisted of twenty nine lung archive paraffin blocks (nineteen sheep and ten goats), which were admitted to Etlik Veterinary Control and Research Institute from Ankara and surrounding provinces with the suspicion of pneumonia between 2015 and 2020. Histopathological findings such as degeneration and desquamation in the bronchi and bronchial epithelium, fibromuscular hypertrophy, hyperplasia in the peribronchial lymphoid tissue, cell infiltration in the interalveolar septum, and no statistical difference was detected in the sheep and goat lung paraffin block tissues (p> 0.05). Immunohistochemically, RSV replication in bronchial and bronchial epithelium and cell debris, bronchial glands, interalveolar septum inflammatory cells, alveolar macrophages was statistically similar in sheep and goats (p> 0.05). It was determined that the expression of TNF-a was more intensely stained in the lung tissue of goats than sheep (p<0.05). In conclusion, it was noticed that RSV infections have an important place in respiratory system infections in sheep and goat herds. It is recommended to carry out studies to detect cytokine storm in natural RSV infections in sheep and goats, and to investigate antibody therapy, which is a new treatment method, in sheep and goat viral infections.

Keywords

• RSV
• TNF-a
• Immunohistochemistry
• Sheep
• Goat

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