Etlik Piliçlerde Göğüs Eti Kas Miyopatileri ve Kreatin Kinaz Enzim Düzeyi Üzerine Genotip ve Yaşın Etkisi

Abstract

Bu çalışma farklı genotip etlik piliçlerde göğüs eti kas miyopatilerinin yaygınlık düzeyi ve serum kreatin kinaz enzim aktivitesini incelemek amacı ile yapılmıştır. Çalışmada Cobb 500, Ross308 ve yerli Anadolu-T genotipi, etlik piliçlerden elde edilen göğüs etleri kullanılmıştır. Her genotipten 50 adet (her tekrarlı grupta 10 adet) olmak üzere toplamda 150 adet, dişi, günlük yaşta, etlik civciv çalışmanın canlı materyalini oluşturmuştur. Deneme gruplarında yer alan hayvanlar; etlik piliçler için standart yöntemler ile tamamı ızgaralı zeminde büyütülmüş, büyütme döneminin 28. gününde kanat venasından, 37 ve 44. günlerinde, kesim esnasında, boyun venasından kan alınarak serum kreatin kinaz aktivitesi ölçülmüştür. Gruplarda 37 ve 44 günlük yaşlarda yapılan kesimlerden el de edilen göğüs eti kas örneklerinde makroskopik olarak derin kas hastalığı, yumuşak-solgun-akışkan göğüs eti, koyu-sert-kuru göğüs eti oluşumu, beyaz çizgi oluşumu, odunumsu göğüs eti ve spagetti et oluşumu gibi göğüs eti kas miyopatileri incelenmiştir. Serum kireatin kinaz enzim düzeyi yaşla birlikte artmış (P<0.001), Ross308 genotipinde önemli düzeyde daha düşük bulunmuştur (P<0.014). İncelenen göğüs kası örneklerinde; kas miyopatilerinden beyaz şerit oluşumu ve odunumsu göğüs kası geliştiği tespit edilmiştir. Kreatin kinaz düzeyi ile ilişkili olarak genelde yaşla birlikte makroskopik miyopati gelişimi de artmıştır. Serum kreatin kinaz düzeyinin göğüs kası miyopatilerinin gelişiminde bir indikatör olarak kullanılabileceği, makroskopik düzeyde genotipler arası farklılığı daha belirgin olarak ortaya koyabilmek için daha fazla sayıda örnek ile çalışılmasının faydalı olacağı sonucuna ulaşılmıştır.

Keywords

• Etlik piliç
• genotip
• kreatin kinaz
• göğüs eti miyopatileri
• et kalitesi

Effects of Genotype and Age on Broiler Breast Meat Myopathies and Serum Creatine Kinase Activity

Abstract

This study was made to investigate serum cratein kinase and breast meat myopathies in female broiler breast meat of local Anadolu-T and two commercial genotypes as RossPM3 and Cobb500. A total of 150-day-old female broiler breast meat and blood samples colllected during slaughter were used. Serum creatine kinase levels were determined by taking blood from the brachial wing vein on the 28th day and from the jugular vein during slaughter on the 37th and 44th days. Deep pectoral myopathie, pale-soft-exudative breast meat, dark-firm-dry breast meat, white striping, wooden breast meat and spaghetti meat formation were examined macroscopically in the breast meat muscle samples obtained from slaughtering at 37 and 44 days of age in the groups. Serum creatine kinase enzyme level increased with age (P<0.001) and was significantly lower in the Ross308 genotype (P<.014). In the examined breast muscle samples, it was determined that white striping and woody breast muscle developed. The prevalence of myopathies increased in relation to the creatine kinase level. It was concluded that serum creatine kinase level can be used as an indicator in the development of pectoral muscle myopathies, and that it would be beneficial to study a larger amount of samples in order to more clearly reveal the differences between genotypes.

Keywords

• Broiler
• genotype
• age
• creatine kinase
• myopathies

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