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Effects of Pregnant Mare Serum Gonadotropine (PMSG) on Synchronisation and Pregnancy Rates of Dairy Cows

Yıl 2022, Cilt: 41 Sayı: 1, 32 - 36, 26.07.2022
https://doi.org/10.30782/jrvm.1110043

Öz

The success rate of artificial insemination depends on, besides other factors, transferring sperm to the genital tract on the right time. Increases on metabolic rates that caused from genetic reclamation have leaded to a decrease in the amounts and effective half-life of the reproductive hormones. This fact reduces the estrous symptoms and causes a decrease on pregnancy rates. As pregnancy is a key factor for production and continuity of the herd, it is the final target to reach in the shortest and the most effective way. For this purpose, fixed time artificial insemination methods (presynch, ovsynch, G6G, G7G, etc.) have been researched over the years. This study aims for replacing the first GnRH injection with PMSG that is being used in ovsynch synchronisation method and research the impacts over estrous symptoms and pregnancy rates. With this design, 139 Simmental dairy cows that are fed, maintained and sheltered under the same conditions were used in the study. Animals that possess production records, primiparus state, sexually active and without a disease history were used in the study. As the result of the study; 61 cows (89.71%) in the PMSG study group that exhibit internal and external estrous signs were found. 55 of these animals (90.16%) were found to be pregnant after artificial inseminations. The animals that were in GnRH study group yielded 56 (78.87%) estrous sign rate and 45 (80.35%) of these animals presented a positive pregnancy after the study. There were no statistical difference found after analysing the results of the study between two groups in terms of occurance of estrous symptoms and pregnancy for the study (P<0.05). These findings presented the results for possibility of replacing the first GnRH injection with PMSG for ovsynch synchronisation method. To became a beacon for the studies in the future, by applying this methods with more animals, further investigations and applications should be put into practice.

Kaynakça

  • Nowicki A, Baranski W, Baryczka A, et al. OvSynch protocol and its modifications in the reproduction management of dairy cattle herds – an update. J Vet Res. 2017;61:329-336. doi: 10.1515/jvetres-2017-0043
  • Lopez-Gatius F, Santolaria P, Mundet I, et al. Walking activity at estrus and subsequent fertility in dairy cows. Theriogenology. 2005;63:1419-1429. doi: 10.1016/j.theriogenology.2004.07.007
  • Firk R, Stamer E, Junge W, et al. Automation of oestrus detection in dairy cows: a review. Livest Prod Sci. 2002;75:219-232. doi: 10.1016/S0301-6226(01)00323-2
  • Sangsritavong S, Combs DK, Sartori R, et al. High Feed Intake Increases Liver Blood Flow and Metabolism of Progesterone and Estradiol-17β in Dairy Cattle. J Dairy Sci 2002;85:2831-2842. doi: 10.3168/jds.S0022-0302(02)74370-1
  • Brozos C, Kiossis E, Hatzieffraimidis S, et al. Comparison of 5 Versus 7-Day Ovsynch + Progesterone Releasing Intravaginal Device Protocols (PRID) and a Modified G7G with an Option of Heat Detection Protocol for 1st Service in Lactating Dairy Cows. Animals. 2021;11;2955. doi: 10.3390/ani11102955
  • Pursley JR, Wiltbank MC, Stevenson JS, et al. Pregnancy rates per artificial insemination for cows and heifers inseminated at a synchronized ovulation or synchronized estrus. J Dairy Sci. 1997;80(2):295-300. doi: 10.3168/jds.S0022-0302(97)75937-X
  • Larson LL ve Ball PHJ. Regulations of estrous cycles in dairy cattle: A review. Theriogenology. 1992;38(2):255-267. doi: 10.1016/0093-691X(92)90234-I
  • Borman JM, Radcliff RP, McCormak BL, et al. Synchronisation of oestrus in dairy cows using prostaglandin F2α, gonadotrophin-releasing hormone, and oestradiol cypionate. Anim Reprod Sci. 2003;76:163-176. doi: 10.1016/S0378-4320(02)00244-0
  • Ribeiro ES, Cerri RL, Bisinotto RS, et al. Reproductive performance of grazing dairy cows following presynchronization and resynchronization protocols. J Dairy Sci. 2011;94:4984-4996. doi: 10.3168/jds.2011-4225
  • Carvalho PD, Guenther JN, Fuenzalida MJ, et al. Presynchronization using a modified Ovsynch protocol or a single gonadotropin-releasing hormone injection 7 d before an Ovsynch-56 protocol for submission of lactating dairy cows to first timed artificial insemination. J Dairy Sci. 2014;97:6305–6315. doi: 0.3168/jds.2014-8222
  • Souza AH, Ayres H, Ferreira RM, et al. A new presynchronization system (Double-Ovsynch) increases fertility at first postpartum timed AI in lactating dairy cows. Theriogenology. 2008;70:208–215. doi: 10.1016/j.theriogenology.2008.03.014
  • Santos VG, Carvalho PD, Maia C, et al. Adding a second prostaglandin F2α treatment to but not reducing the duration of a PRID-Synch protocol increases fertility after resynchronization of ovulation in lactating Holstein cows. J Dairy Sci. 2016;99:3869–3879. doi: 10.3168/jds.2015-10557
  • Pursley JR, Mee MO, Wiltbank MC. Synchronization of ovulation in dairy cows using PGF2α and GnRH. Theriogenology. 1995;44:915–923.
  • De Rensis F ve Lopez-Gatius F. Use of Equine Chorionic Gonadotropin to Control Reproduction of the Dairy Cow: A Review. Reprod Domest Anim. 2014;49:177-182. doi: 10.1111/rda.12268
  • Gonzalez A, Wang H, Carruthers TD, et al. Superovulation in the cow with pregnant mare serum gonadotrophin: Effects of dose and antipregnant mare serum gonadotrophin serum. Can Vet J. 1994;35:158-162.
  • Praharani L, Sianturi RSG, Kusimaningrum DA, et al. Superovulatory responses using pregnant mare serum gonadotropin hormone in Murrah buffalo cows. 2nd International Conference on Animal Production for Food Sustainability. 2021;888:012016. http://conference.faterna.unand.ac.id/2021/ doi: 10.1088/1755-1315/888/1/012016
  • Santos VG, Carvalho PD, Maia C, et al. Adding a second prostaglandin F2α treatment to but not reducing the duration of a PRID-Synch protocol increases fertility after resynchronization of ovulation in lactating Holstein cows. J Dairy Sci. 2015;99:3869-3879.
  • Yılmazbaş-Mecitoğlu G, Karakaya E, Keskin A, et al. Comparison of synchronisation and fertility after different modifications of the ovsynch protocol in cyclic dairy cows. Acta Vet Hung. 2014;62(1):64-73.
  • Bisinotto RS, Pansani MB, Castro LO, et al. Effect of progesterone supplementation on fertility responses of lactating dairy cows with corpus luteum at the initiation of the Ovsynch protocol. Theriogenology. 2015;83:257-265.
  • Folman Y, Rosenberg M, Herz Z, et al. The relationship between plasma progesterone concentration and conception in post-partum dairy cows maintained on two levels of nutrition. J Reprod Fertil. 1973;34:267-278.
  • Meisterling EM ve Dailey RA. Use of concentrations of progesterone and estradiol-17ß in milk in monitoring postpartum ovarian function in dairy cows. J Dairy Sci. 1987;70:2154-2161.
  • Wiltbank MC, Souza AH, Carvalho PD, et al. Improving fertility to timed artificial insemination by manipulation of circulating progesterone concentrations in lactating dairy cattle. Reprod Fertil Dev. 2012;24:238-243.
  • Alnimer M ve Lubbadeh W. Effect of using progesterone releasing intravaginal device with ovsynch program on reproduction in dairy cattle during summer season. Anim Biosci. 2003;16(9):1268-1273.
  • Kuru M, Kacar C, Oral H, et al. Effect of two prostaglandin F2α injections administered 24 hours apart on the pregnancy rate of Simmental cows subjected to the Ovsynch or Ovsynch + Controlled internal drug release (CIDR) protocols. Med Veter. 2020;76(11):660-665.
  • Silva LACL, Simoes LMS, Bottine MP, et al. Presynchronization by induction of a largest follicle using a progesterone device in GnRH-based-ovulation synchronization protocol in crossbred dairy cows. Theroigenology. 2018;119:233-237.
  • Garcia-Pintos C ve Menchaca A. Pregnancy establishment and maintenance after the administration of equine chorionic gonadotropin (eCG) associated or not with gonadotropin-releasing hormone (GnRH) after insemination in sheep. Anim Prod Sci. 2018;58:1802-1806.
  • Kuru M, Boga Kuru B, Sogukpinar O, et al. Oestrus synchronisation with progesterone-containing sponge and equine chorionic gonadotropin in Pirlak ewes during the non-breeding season: can Toryum improve fertility parameters?. J Vet Res. 2020;64:573-579.
  • Zarkawi M. Oestrous synchronisation and twinning rate of Syrian Awassi ewes treated with progestagen and PMSG during the breeding season. New Zealand Journal of Agricultural Research. 2001;44:159-163.
  • Keskin M. Influence of buck effect and exogenous hormone treatments on oestrus synchronisation and litter size in shami (damascus) goats. Turk J Vet Anim Sci.. 2003;27:453-457.
  • Gabor GY, Kastelic JP, Pinter S, et al. Improving reproductive performance in lactating dairy cows by synchronising ovulation or inducing oestrus. Acta Vet Hung. 2002;50(2):231-234.
  • Grimard B, Humblot P, Mialot JP, et al. Absence of response to oestrus induction and synchronization treatment is related to lipid mobilization in suckled beef cows. Reprod Nutr Dev. 1997;37:129-140.
  • Macmillan KL ve Burke CR. Effects of oestrous cycle control on reproductive efficiency. Anim Reprod Sci. 1996;42:307-320.

Gebe Kısrak Serum Gonadotropini (PMSG)’nin Sütçü İneklerde Senkronizasyon ve Gebelik Oranları Üzerine Etkileri

Yıl 2022, Cilt: 41 Sayı: 1, 32 - 36, 26.07.2022
https://doi.org/10.30782/jrvm.1110043

Öz

Suni tohumlama yönteminin başarısı, birçok diğer etken ile birlikte, uygulayıcının spermayı doğru zamanda genital kanala vermesinden geçmektedir. Süt hayvancılığında ıslah sonucu meydana gelen metabolizma artışı, üreme hormonlarının miktarında ve yarılanma sürelerinde değişikliklere sebep olmaktadır. Bu durum, östrus bulgularının görülmesinde ve dolayısıyla elde edilecek gebelik sonuçlarının doğrudan azalmasına neden olmaktadır. Sürü devamlılığı ve üretim verimliliği açısından son derece önemli olan gebelik, en ideal ve kısa yoldan ulaşılması gereken nihai hedeftir. Bu amaçla sabit zamanlı suni tohumlama için kullanılan yöntemler (presynch, ovsynch, G6G, G7G, v.b.) yıllar boyunca araştırılmış ve günümüzde geliştirilmeye devam etmektedir. Bu çalışmada, progesteron destekli ovsynch senkronizasyonu ile aynı planının ilk hormonu olan GnRH enjeksiyonu yerine PMSG ile gerçekleştirilen indüksiyon sonrasında elde edilecek östrus cevapları ve buna bağlı olarak elde edilecek gebelik oranlarının araştırılması planlanmıştır. Bu amaçla ticari olarak süt üretimi yapan entansif bir işletmede, aynı koşullarda bakım, besleme ve barındırılması gerçekleştirilen toplam 139 baş sütçü Simmental inek kullanıldı. Çalışmaya dâhil edilen hayvanlar; üretim kayıtları bilinen, düzenli olarak cinsel aktivite gösteren, en az bir doğum geçmişi olan ve herhangi bir hastalık geçmişi bulunmayan hayvanlar arasından seçildi. Çalışma sonucunda, PMSG grubuna hem iç hem de dış östrus bulguları gösteren hayvanların sayısı 61 baş olarak, %89,71 oranında tespit edilmiş ve bu hayvanların da 55 tanesinde gebelik sonucu elde edilmiştir (%90,16). GnRH ile başlanan senkronizasyon grubunda östrus yanıtı 56 başta (%78,87) tespit edilmiş ve bu hayvanlardan elde edilen gebelik 45 baş hayvan ile %80,35 oranında bulunmuştur. Gerçekleştirilen istatistiki analiz sonucunda, iki senkronizasyon modelinde de östrus bulgularının gözlemlenmesi ve östrusa bağlı olarak elde edilen gebelik sonuçları arasında istatistiki anlamlılık değerinde fark bulunamamıştır (P<0,05). Bu sonuçlar, ovsynch yönteminde kullanılan ilk GnRH yerine PMSG’nin başarı ile kullanılabileceğini ortaya koymaktadır. Gerçekleştirilen bu çalışma ile, PMSG kullanılarak planlanacak çeşitli senkronizasyon yöntemlerinin daha fazla hayvana uygulanması ve elde edilecek yeni metotlara yol gösterici nitelikte olacağı öngörülmektedir.

Kaynakça

  • Nowicki A, Baranski W, Baryczka A, et al. OvSynch protocol and its modifications in the reproduction management of dairy cattle herds – an update. J Vet Res. 2017;61:329-336. doi: 10.1515/jvetres-2017-0043
  • Lopez-Gatius F, Santolaria P, Mundet I, et al. Walking activity at estrus and subsequent fertility in dairy cows. Theriogenology. 2005;63:1419-1429. doi: 10.1016/j.theriogenology.2004.07.007
  • Firk R, Stamer E, Junge W, et al. Automation of oestrus detection in dairy cows: a review. Livest Prod Sci. 2002;75:219-232. doi: 10.1016/S0301-6226(01)00323-2
  • Sangsritavong S, Combs DK, Sartori R, et al. High Feed Intake Increases Liver Blood Flow and Metabolism of Progesterone and Estradiol-17β in Dairy Cattle. J Dairy Sci 2002;85:2831-2842. doi: 10.3168/jds.S0022-0302(02)74370-1
  • Brozos C, Kiossis E, Hatzieffraimidis S, et al. Comparison of 5 Versus 7-Day Ovsynch + Progesterone Releasing Intravaginal Device Protocols (PRID) and a Modified G7G with an Option of Heat Detection Protocol for 1st Service in Lactating Dairy Cows. Animals. 2021;11;2955. doi: 10.3390/ani11102955
  • Pursley JR, Wiltbank MC, Stevenson JS, et al. Pregnancy rates per artificial insemination for cows and heifers inseminated at a synchronized ovulation or synchronized estrus. J Dairy Sci. 1997;80(2):295-300. doi: 10.3168/jds.S0022-0302(97)75937-X
  • Larson LL ve Ball PHJ. Regulations of estrous cycles in dairy cattle: A review. Theriogenology. 1992;38(2):255-267. doi: 10.1016/0093-691X(92)90234-I
  • Borman JM, Radcliff RP, McCormak BL, et al. Synchronisation of oestrus in dairy cows using prostaglandin F2α, gonadotrophin-releasing hormone, and oestradiol cypionate. Anim Reprod Sci. 2003;76:163-176. doi: 10.1016/S0378-4320(02)00244-0
  • Ribeiro ES, Cerri RL, Bisinotto RS, et al. Reproductive performance of grazing dairy cows following presynchronization and resynchronization protocols. J Dairy Sci. 2011;94:4984-4996. doi: 10.3168/jds.2011-4225
  • Carvalho PD, Guenther JN, Fuenzalida MJ, et al. Presynchronization using a modified Ovsynch protocol or a single gonadotropin-releasing hormone injection 7 d before an Ovsynch-56 protocol for submission of lactating dairy cows to first timed artificial insemination. J Dairy Sci. 2014;97:6305–6315. doi: 0.3168/jds.2014-8222
  • Souza AH, Ayres H, Ferreira RM, et al. A new presynchronization system (Double-Ovsynch) increases fertility at first postpartum timed AI in lactating dairy cows. Theriogenology. 2008;70:208–215. doi: 10.1016/j.theriogenology.2008.03.014
  • Santos VG, Carvalho PD, Maia C, et al. Adding a second prostaglandin F2α treatment to but not reducing the duration of a PRID-Synch protocol increases fertility after resynchronization of ovulation in lactating Holstein cows. J Dairy Sci. 2016;99:3869–3879. doi: 10.3168/jds.2015-10557
  • Pursley JR, Mee MO, Wiltbank MC. Synchronization of ovulation in dairy cows using PGF2α and GnRH. Theriogenology. 1995;44:915–923.
  • De Rensis F ve Lopez-Gatius F. Use of Equine Chorionic Gonadotropin to Control Reproduction of the Dairy Cow: A Review. Reprod Domest Anim. 2014;49:177-182. doi: 10.1111/rda.12268
  • Gonzalez A, Wang H, Carruthers TD, et al. Superovulation in the cow with pregnant mare serum gonadotrophin: Effects of dose and antipregnant mare serum gonadotrophin serum. Can Vet J. 1994;35:158-162.
  • Praharani L, Sianturi RSG, Kusimaningrum DA, et al. Superovulatory responses using pregnant mare serum gonadotropin hormone in Murrah buffalo cows. 2nd International Conference on Animal Production for Food Sustainability. 2021;888:012016. http://conference.faterna.unand.ac.id/2021/ doi: 10.1088/1755-1315/888/1/012016
  • Santos VG, Carvalho PD, Maia C, et al. Adding a second prostaglandin F2α treatment to but not reducing the duration of a PRID-Synch protocol increases fertility after resynchronization of ovulation in lactating Holstein cows. J Dairy Sci. 2015;99:3869-3879.
  • Yılmazbaş-Mecitoğlu G, Karakaya E, Keskin A, et al. Comparison of synchronisation and fertility after different modifications of the ovsynch protocol in cyclic dairy cows. Acta Vet Hung. 2014;62(1):64-73.
  • Bisinotto RS, Pansani MB, Castro LO, et al. Effect of progesterone supplementation on fertility responses of lactating dairy cows with corpus luteum at the initiation of the Ovsynch protocol. Theriogenology. 2015;83:257-265.
  • Folman Y, Rosenberg M, Herz Z, et al. The relationship between plasma progesterone concentration and conception in post-partum dairy cows maintained on two levels of nutrition. J Reprod Fertil. 1973;34:267-278.
  • Meisterling EM ve Dailey RA. Use of concentrations of progesterone and estradiol-17ß in milk in monitoring postpartum ovarian function in dairy cows. J Dairy Sci. 1987;70:2154-2161.
  • Wiltbank MC, Souza AH, Carvalho PD, et al. Improving fertility to timed artificial insemination by manipulation of circulating progesterone concentrations in lactating dairy cattle. Reprod Fertil Dev. 2012;24:238-243.
  • Alnimer M ve Lubbadeh W. Effect of using progesterone releasing intravaginal device with ovsynch program on reproduction in dairy cattle during summer season. Anim Biosci. 2003;16(9):1268-1273.
  • Kuru M, Kacar C, Oral H, et al. Effect of two prostaglandin F2α injections administered 24 hours apart on the pregnancy rate of Simmental cows subjected to the Ovsynch or Ovsynch + Controlled internal drug release (CIDR) protocols. Med Veter. 2020;76(11):660-665.
  • Silva LACL, Simoes LMS, Bottine MP, et al. Presynchronization by induction of a largest follicle using a progesterone device in GnRH-based-ovulation synchronization protocol in crossbred dairy cows. Theroigenology. 2018;119:233-237.
  • Garcia-Pintos C ve Menchaca A. Pregnancy establishment and maintenance after the administration of equine chorionic gonadotropin (eCG) associated or not with gonadotropin-releasing hormone (GnRH) after insemination in sheep. Anim Prod Sci. 2018;58:1802-1806.
  • Kuru M, Boga Kuru B, Sogukpinar O, et al. Oestrus synchronisation with progesterone-containing sponge and equine chorionic gonadotropin in Pirlak ewes during the non-breeding season: can Toryum improve fertility parameters?. J Vet Res. 2020;64:573-579.
  • Zarkawi M. Oestrous synchronisation and twinning rate of Syrian Awassi ewes treated with progestagen and PMSG during the breeding season. New Zealand Journal of Agricultural Research. 2001;44:159-163.
  • Keskin M. Influence of buck effect and exogenous hormone treatments on oestrus synchronisation and litter size in shami (damascus) goats. Turk J Vet Anim Sci.. 2003;27:453-457.
  • Gabor GY, Kastelic JP, Pinter S, et al. Improving reproductive performance in lactating dairy cows by synchronising ovulation or inducing oestrus. Acta Vet Hung. 2002;50(2):231-234.
  • Grimard B, Humblot P, Mialot JP, et al. Absence of response to oestrus induction and synchronization treatment is related to lipid mobilization in suckled beef cows. Reprod Nutr Dev. 1997;37:129-140.
  • Macmillan KL ve Burke CR. Effects of oestrous cycle control on reproductive efficiency. Anim Reprod Sci. 1996;42:307-320.
Toplam 32 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Konular Veteriner Cerrahi
Bölüm Research Articles
Yazarlar

Mehmed Berk Toker 0000-0003-4033-9749

Selim Alçay 0000-0002-2472-8157

Yayımlanma Tarihi 26 Temmuz 2022
Kabul Tarihi 18 Mayıs 2022
Yayımlandığı Sayı Yıl 2022 Cilt: 41 Sayı: 1

Kaynak Göster

APA Toker, M. B., & Alçay, S. (2022). Gebe Kısrak Serum Gonadotropini (PMSG)’nin Sütçü İneklerde Senkronizasyon ve Gebelik Oranları Üzerine Etkileri. Journal of Research in Veterinary Medicine, 41(1), 32-36. https://doi.org/10.30782/jrvm.1110043
AMA Toker MB, Alçay S. Gebe Kısrak Serum Gonadotropini (PMSG)’nin Sütçü İneklerde Senkronizasyon ve Gebelik Oranları Üzerine Etkileri. J Res Vet Med. Temmuz 2022;41(1):32-36. doi:10.30782/jrvm.1110043
Chicago Toker, Mehmed Berk, ve Selim Alçay. “Gebe Kısrak Serum Gonadotropini (PMSG)’nin Sütçü İneklerde Senkronizasyon Ve Gebelik Oranları Üzerine Etkileri”. Journal of Research in Veterinary Medicine 41, sy. 1 (Temmuz 2022): 32-36. https://doi.org/10.30782/jrvm.1110043.
EndNote Toker MB, Alçay S (01 Temmuz 2022) Gebe Kısrak Serum Gonadotropini (PMSG)’nin Sütçü İneklerde Senkronizasyon ve Gebelik Oranları Üzerine Etkileri. Journal of Research in Veterinary Medicine 41 1 32–36.
IEEE M. B. Toker ve S. Alçay, “Gebe Kısrak Serum Gonadotropini (PMSG)’nin Sütçü İneklerde Senkronizasyon ve Gebelik Oranları Üzerine Etkileri”, J Res Vet Med, c. 41, sy. 1, ss. 32–36, 2022, doi: 10.30782/jrvm.1110043.
ISNAD Toker, Mehmed Berk - Alçay, Selim. “Gebe Kısrak Serum Gonadotropini (PMSG)’nin Sütçü İneklerde Senkronizasyon Ve Gebelik Oranları Üzerine Etkileri”. Journal of Research in Veterinary Medicine 41/1 (Temmuz 2022), 32-36. https://doi.org/10.30782/jrvm.1110043.
JAMA Toker MB, Alçay S. Gebe Kısrak Serum Gonadotropini (PMSG)’nin Sütçü İneklerde Senkronizasyon ve Gebelik Oranları Üzerine Etkileri. J Res Vet Med. 2022;41:32–36.
MLA Toker, Mehmed Berk ve Selim Alçay. “Gebe Kısrak Serum Gonadotropini (PMSG)’nin Sütçü İneklerde Senkronizasyon Ve Gebelik Oranları Üzerine Etkileri”. Journal of Research in Veterinary Medicine, c. 41, sy. 1, 2022, ss. 32-36, doi:10.30782/jrvm.1110043.
Vancouver Toker MB, Alçay S. Gebe Kısrak Serum Gonadotropini (PMSG)’nin Sütçü İneklerde Senkronizasyon ve Gebelik Oranları Üzerine Etkileri. J Res Vet Med. 2022;41(1):32-6.