Biomass Production by Yarrowia lipolytica from Olive Mill Wastewater: Evaluation of Protein Content
Abstract
Olive mill wastewater (OMW), a by-product of olive oil production, is a promising substrate for microbial applications. This agro-industrial waste offers considerable potential for biomass production as it contains carbon and nitrogen sources that support microbial growth. Additionally, utilization of this waste contributes to environmentally sustainable waste management. Utilizing OMW as a growth medium, Yarrowia lipolytica offers an alternative to synthetic media, enhancing the economic value of this waste while reducing production costs. In this study, biomass production by Y. lipolytica from OMW was optimized using the Taguchi method. The experimental design evaluated the effects of the OMW dilution rate (20, 40, and 60%), nitrogen concentration (0, 1, and 2 g/L), and incubation time (3, 5, and 7 days). Nine experiments were conducted using the L9 orthogonal array, and significant factors were identified using analysis of variance (ANOVA). Among these variables, nitrogen concentration significantly influenced biomass production (p < 0.05). The highest biomass concentration of 11.58±0.93 g/L was achieved, and the optimum conditions were found as OMW dilution rate of 60%, 2 g/L nitrogen addition, and 5 days of incubation. Under these conditions, the protein content of the biomass was determined as 25.88±0.63% w/w biomass dry weight.
Keywords
olive mill wastewater, biomass, Yarrowia, waste management, Taguchi
References
- D. Sarris, A. Rapti, N. Papafotis, A. A. Koutinas, and S. Papanikolaou, "Production of added-value chemical compounds through bioconversions of olive-mill wastewaters blended with crude glycerol by a Yarrowia lipolytica strain," Molecules, vol. 24, no. 2, p. 222, 2019.
- F. Chidichimo, M. R. Basile, C. Conidi, G. De Filpo, R. Morelli, and A. Cassano, "A new approach for bioremediation of olive mill wastewaters: combination of straw filtration and nanofiltration," Membranes, vol. 14, no. 2, p. 38, 2024.
- K. Ayadi, M. Meziane, D. Rouam, M. N. Bouziane, and K. El-Miloudi, "Olive mill wastewater for bioethanol production using immobilised cells," Kemija u industriji, vol. 71, no. 1-2, pp. 21–28, 2022.
- M. R. Zahi, W. Zam, and M. El Hattab, "State of knowledge on chemical, biological and nutritional properties of olive mill wastewater," Food Chemistry, vol. 381, p. 132238, 2022.
- G. J. Martin and S. Chan, "Future production of yeast biomass for sustainable proteins: a critical review," Sustainable Food Technology, 2024.
- D. I. Koukoumaki et al., "Recent advances in the production of single cell protein from renewable resources and applications," Carbon Resources Conversion, vol. 7, no. 2, p. 100195, 2024.
- M. Sharif et al., "Single cell protein: Sources, mechanism of production, nutritional value and its uses in aquaculture nutrition," Aquaculture, vol. 531, p. 735885, 2021.
- B. C. Bratosin, S. Darjan, and D. C. Vodnar, "Single cell protein: A potential substitute in human and animal nutrition," Sustainability, vol. 13, no. 16, p. 9284, 2021.
- P. Juszczyk, W. Rymowicz, A. Kita, and A. Rywińska, "Biomass production by Yarrowia lipolytica yeast using waste derived from the production of ethyl esters of polyunsaturated fatty acids of flaxseed oil," Industrial Crops and Products, vol. 138, p. 111590, 2019.
- R. Yang, Z. Chen, P. Hu, S. Zhang, and G. Luo, "Two-stage fermentation enhanced single-cell protein production by Yarrowia lipolytica from food waste," Bioresource Technology, vol. 361, p. 127677, 2022.